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Association between mutation of the NF2gene and monosomy 22 in menopausal women with sporadic meningiomas
© Tabernero et al.; licensee BioMed Central Ltd. 2013
Received: 28 June 2013
Accepted: 28 October 2013
Published: 30 October 2013
Meningioma was the first solid tumor shown to contain a recurrent genetic alteration e.g. monosomy 22/del(22q), NF2 being the most relevant gene involved. Although monosomy 22/del(22q) is present in half of all meningiomas, and meningiomas frequently carry NF2 mutations, no study has been reported so far in which both alterations are simultaneously assessed and correlated with the features of the disease.
Here, we analyzed the frequency of both copy number changes involving chromosome 22 and NF2 mutations in 20 sporadic meningiomas using high-density SNP-arrays, interphase-FISH and PCR techniques.
Our results show a significant frequency of NF2 mutations (6/20 patients, 30%), most of which (5/6) had not been previously reported in sporadic meningiomas. NF2 mutations involved five different exons and led to a truncated protein (p.Leu163CysfsX46, p.Phe62LeufsX61, p.Asp281MetfsX15, p.Phe285LeufsX11, p.Gln389ArgfsX37) and an in frame deletion of Phe119. Interestingly, all NF2 mutated cases were menopausal women with monosomy 22 but not del(22q).
These results confirm and extend on previous observations about the high frequency and heterogeneity of NF2 mutations in sporadic meningiomas and indicate they could be restricted to a well-defined cytogenetic and clinical subgroup of menopausal women. Further studies in large series of patients are required to confirm our observations.
Meningioma was the first type of solid tumor which already in 1967, was shown to contain a specific recurrent genetic alteration consisting of loss of a chromosome 22 in around half of the cases . The association observed between the dominantly-inherited neurofibromatosis type-2 syndrome and central nervous system (CNS) tumors such as meningiomas and schwannomas, has rapidly led to the identification of the NF2 gene located in chromosome 22q as a candidate predisposing gene in both familial and sporadic meningiomas [2, 3]. However, loss of chromosome 22 and/or del(22q) are only found in a fraction of all meningiomas suggesting that molecular and chromosomal changes other than those targeting the NF2 gene may also be involved in the development of meningiomas [4, 5]. Since the earliest reports till 1994, more than 400 different NF2 mutations have been described in meningiomas [6–27]; however, the precise significance of the NF2 gene status in sporadic meningiomas remains unclear. Based on sequencing data from the literature, NF2 mutations may involve all exons of the gene except exon 16. Interestingly, an association between NF2 gene mutations and specific subtypes of meningiomas such as fibroblastic and transitional tumors has been observed [7–9, 24], although such data could not be always confirmed in other series .
In this study, we report on the frequency of NF2 mutations in a group of 20 sporadic WHO grade I/benign meningiomas and its association with the distinct genetic and histopathological subtypes of meningiomas. At the same time, we also review the literature reported so far on NF2 mutations in meningiomas. Overall, six distinct NF2 mutations were found (6/20 cases; 30%) five of which had not been previously reported in sporadic meningiomas [10, 24, 29]. Noteworthy, NF2-mutated tumors were systematically associated with complete loss of chromosome 22 (monosomy 22) but not del(22q), usually in the absence of additional genetic alterations involving other chromosomes as assessed by both interphase fluorescence in situ hybridization (iFISH) and single nucleotide polymorphism (SNP) arrays. These results, together with the higher median age and the slightly greater frequency of transitional meningiomas, among the mutated cases, suggest that NF2-mutated tumors may represent a uniquely well-defined subgroup of sporadic meningiomas.
Patients and samples
A total of 20 adult WHO grade I (sporadic) meningioma patients (3 males and 17 females; mean age of 60 ± 16 years) were included in this study. Prior to entering the study, informed consent was given by each individual and the study was approved by the ethics committee of the University Hospital of Salamanca (Salamanca, Spain). Classification of meningioma was established according to the WHO criteria, with the following distribution: meningothelial meningiomas, 10 cases; transitional, 7, and; fibroblastic meningiomas, 3 tumors. In each individual patient, paired fresh EDTA-anticoagulated peripheral blood (PB) and tumoral samples were obtained in parallel and stored in liquid nitrogen (−150°C), until analysed.
Both tumoral and PB cells’ DNA was extracted using the QIAamp DNA mini kit (Qiagen, Hilden, Germany) following the instructions of the manufacturer. A NanoDrop-1000 Spectrophotometer (Nano-Drop Technologies, Wilmington, DE, USA) was used to quantify the amount of DNA obtained and assess its quality.
Copy number alterations and loss of heterozygosity (LOH) of chromosome 22 by iFISH and SNP-arrays
In order to confirm the presence of numerical changes involving chromosome 22, iFISH studies were performed with commercially available probes for chromosome 9 and 22q (LSI bcr/abl ES DC Probe). In addition, in 15/20 patients copy number alterations and LOH were analyzed with the GeneChip Human Mapping 250 K Nsp and 250 K Sty-arrays (Affymetrix), as previously reported .
Identification of NF2mutations
DNA from the 20 meningioma samples was amplified by conventional PCR . In order to identify mutations in the NF2 gene sequence, 32 customized primers were used. Oligonucleotide primer sequences were obtained from the UniSTS database at NCBI (http://www.ncbi.nlm.nih.gov).
PCR products were analysed by capillary electrophoresis using an ABI 3130xl instrument (Applied Biosystems, Foster City, CA, USA) and the Chromas (Technelysium Pty Ltd, http://technelysium.com.au) software was used to analyze the DNA sequences obtained.
The statistical significance of differences observed between groups was assessed by the Student T and the Mann–Whitney U tests, for parametric and non-parametric (continuous) variables, respectively; for qualitative variables, the X 2 test was used (SPSS software, SPSS 15.0, SPSS Inc, Chicago, IL, USA). P-values <0.05 were considered to be associated with statistical significance.
Frequency, localization and type of NF2mutations in sporadic meningiomas
Clinical, histopathological and genetic characteristics of meningioma patients grouped according to the presence vs absence of NF2 gene mutations (n = 20)
(n = 6)
(n = 14)
Age (median in years)
Chromosome 22 status by iFISH
Isolated monosomy 22
Complex-karyotype: with monosomy 22
Complex-karyotype w/o −22/del(22q)
Association between NF2mutations and other chromosomal changes
From the 20 meningiomas analyzed, 7 showed a diploid karyotype (35%), another 6 (30%) had an isolated loss of chromosome 22, two or more chromosomal changes including loss of chromosome 22q were found in another 5 cases –monosomy 22 in 3 (15%) and del(22q) in the other 2 patients (10%)– and two meningiomas displayed multiple chromosomal losses/gains in the absence of monosomy 22/del(22q) (n = 2; 10%) (Table 1).
Interestingly, all 6 NF2-mutated tumors carried monosomy 22, which was the only chromosomal alteration in 4/6 cases. Consequently, NF2-mutated meningiomas accounted for most cases associated with monosomy 22 (6/9; 67%), including cases with isolated monosomy 22 (4/6; 67%) or with monosomy 22 combined with other chromosomal alterations (2/6, 33%); conversely, among all other cases except three, which were either diploid for chromosome 22, carried del(22q) or had multiple chromosomal losses/gains in the absence of monosomy 22/del(22q), showed no NF2 mutations (0/11; p = 0.03)(Table 1).
Chromosome 22 copy number alterations and LOH by SNP-arrays
Chromosome 22 status by iFISH vs SNP-arrays performed in 15/20 sporadic meningiomas
Chromosome 22 status by SNP arrays* (n = 6206)
Chromosome 22 status
Copy number values
N. of SNPs with LOH/N. of informative SNPs (%)
/ 1326 (8%)
/ 1131 (32%)
Complex & -22q
/ 1158 (39%)
/ 1472 (5%)
/ 1188 (77%)
Complex & -22q
/ 1043 (67%)
/ 1340 (16%)
/ 1505 (1%)
Complex & del(22q)
/ 1683 (2%)
Complex & del(22q)
/ 1195 (64%)
/ 1630 (0.4%)
/ 1770 (0.5%)
/ 1712 (0.2%)
/ 1681 (0.3%)
/ 1696 (0.2%)
Association between NF2mutations and other features of the disease
Interestingly, all NF2-mutated meningiomas corresponded to female patients (6/6 vs 11/14, p > 0.05) with a higher median age vs all other cases (73 vs 53 years; p = 0.03)(Table 1). By contrast, a similar localization pattern was observed for the 6 NF2-mutated tumors and the other 14 non-mutated meningiomas (Table 1).
From the histopathological point of view, the 6 NF2 mutated benign/grade I meningiomas showed a variable histology including 3 transitional meningiomas, two meningothelial tumors and one fibroblastic tumor, the frequency of transitional tumors being slightly higher than among non-mutated cases (3/6 vs 4/14 cases; p > 0.05) (Table 1).
Many studies have confirmed that loss of genetic material from chromosome 22 is by far the most frequent chromosomal alteration in meningiomas, being detected in around half of the cases. Such losses of chromosome 22 are heterogeneous and while they most frequently involve the whole chromosome (monosomy 22), in another substantial proportion of cases it consists of del(22q) with variable breakpoints, both alterations –monosomy 22 and del(22q) − being found either as the only chromosomal alteration or in combination with distinct alterations involving other chromosomes [30, 31]. In parallel, multiple studies have also shown a variable frequency (between 14% and 78% of the cases) of NF2 gene mutations in heterogeneous series of meningiomas consisting of between 12 to 170 patients [18, 27], some of these reports specifically focusing on the investigation of particular subsets of patients such as recurrent meningiomas , rare histopathological subtypes  or patients affected by multiple meningiomas . Recurrent chromosomal losses deviating from normal gene dosage present in tumor cells of various types, frequently point out the existence of one or more underlying tumor suppressor genes coded in the deleted DNA sequences. Because of this and the development of meningiomas in patients with neurofibromatosis carrying mutations of the NF2 gene coded at chromosome 22q12.2, NF2 has long been considered as the most relevant gene targeted by chromosome 22 losses in meningiomas [7, 32]. However, careful analysis of the studies reported so far reveals that the association between chromosome 22 status and the presence of NF2 mutations has not been investigated in detail in sporadic meningiomas.
In the present study, we demonstrate the existence of a close association between NF2 mutation and monosomy 22 but not del(22q), in sporadic meningiomas. Whereas most cases with monosomy 22 showed NF2 mutations, these were absent in all three patients who showed del(22q). Altogether, these findings suggest that the NF2 gene would only be involved in the pathogenesis of a well-defined subset of all meningiomas, typically in association with monosomy 22. Of note, a few cases showed monosomy 22 in the absence of NF2 mutations; these results support previous observations indicating that in meningiomas, monosomy 22 is typically found at higher frequencies than NF2 mutations, although the frequency of both alterations also depends on the detection method. This, together with the observation that NF2 mutations were always present in heterozygosis, could suggest that mutations of the NF2 gene could be a secondary event in the development of meningiomas which would occur after the loss of chromosome 22 and that therefore, inactivation of other genes coded in chromosome 22 (e.g. SMARCB1) could potentially play a major role in tumor development, even among NF2 mutated cases. In line with this hypothesis, NF2- mutated meningiomas also showed a rather low incidence of LOH of the NF2 gene by SNP-arrays. However, other mechanisms e.g. rearrangements identifiable by MPLA and other techniques, together with mutations in promoter and other non coding regions, and epigenetic mechanisms leading to inactivation of NF2 gene expression, may also be involved and should be investigated to confirm or rule out this hypothesis. Of note, among cases with monosomy 22/del(22q) in the absence of NF2 mutations, two patients showed lack of LOH, which could be related to the greater percentage of non-tumoral diploid cells present in both patients (data not shown) .
Despite all the above, detailed analysis of the clinical and histopathological features of the meningiomas patients studied at diagnosis, showed unique features for those sporadic tumors carrying NF2-mutations in association with monosomy 22. Accordingly, these cases systematically corresponded to older menopausal women, they more frequently showed a meningothelial histopathological appearance, and none of them had relapsed so far. These results suggest that NF2-mutated sporadic meningiomas may correspond to a unique cytogenetic and clinical subtype of meningiomas, which emerge at relatively advanced ages. In line with this hypothesis, previous studies also suggested the existence of a relationship between NF2 gene mutations and the histology of meningiomas (e.g. transitional tumors ); however, this association remains controversial and in our series, the association between NF2 mutated and transitional meningiomas did not reach statistical significance, in line with the findings of several groups [7, 8, 21].
Frequency of NF2-gene mutations in our patients and other series from the literature reporting >10 sporadic meningiomas
No of mutated cases/total cases (%)
Ruttledge, et al., 1994 **
LeKanne Deprez, et al., 1994 **
Merel, et al., 1995 **
Wellenreuther, et al., 1995 
Ng, et al., 1995 
Papi, et al., 1995 **
Harada, et al., 1996 
Ruttledge, et al., 1996 
De Vitis, et al., 1996 
Stangl, et al., 1997 
Leone, et al., 1999 **
Ueki, et al., 1999 
Evans, et al., 2001 
Joachim, et al., 2001 *
Szijan, et al., 2003 
Lomas, et al., 2005 **
Hartmann, et al., 2006 #
Kim, et al., 2006 **
Hansson, et al., 2007 **
Goutagny, et al., 2010 &
Tabernero et al. (2013)
In the present study we confirm the relatively high frequency of distinct NF2 mutations leading to a truncated protein in sporadic meningiomas, most of the identified genetic changes corresponding to mutations which had not been previously described. Interestingly, a clear association was found among our cases between mutation of the NF2 gene and monosomy 22, but not del(22q); in addition, all NF2-mutated cases corresponded to older menopausal women, supporting the notion that NF2-mutated patients could represent a well-defined and unique cytogenetic and clinical subtype of meningiomas in which acquisition of monosomy 22 could be sequentially followed by NF2 mutation during the development of the disease. Further studies in large series of meningioma patients are required to confirm these observations.
This work has been partially supported by grants from Consejeria Sanidad Junta de Castilla y León, Gerencia Regional de Salud: GRS689/A/11. Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo, Madrid, Spain: RTICC RD06/0020/0035, RD06/0020/0059 and RD12/0036/0048.
MD Tabernero is supported by IECSCYL (Fundación Instituto de Estudios Ciencias de la Salud de Castilla y León).
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