Novel de novo BRCA2mutation in a patient with a family history of breast cancer
© Hansen et al; licensee BioMed Central Ltd. 2008
Received: 26 January 2008
Accepted: 02 July 2008
Published: 02 July 2008
BRCA2 germ-line mutations predispose to breast and ovarian cancer. Mutations are widespread and unclassified splice variants are frequently encountered. We describe the parental origin and functional characterization of a novel de novo BRCA2 splice site mutation found in a patient exhibiting a ductal carcinoma at the age of 40.
Variations were identified by denaturing high performance liquid chromatography (dHPLC) and sequencing of the BRCA1 and BRCA2 genes. The effect of the mutation on splicing was examined by exon trapping in COS-7 cells and by RT-PCR on RNA isolated from whole blood. The paternity was determined by single nucleotide polymorphism (SNP) microarray analysis. Parental origin of the de novo mutation was determined by establishing mutation-SNP haplotypes by variant specific PCR, while de novo and mosaic status was investigated by sequencing of DNA from leucocytes and carcinoma tissue.
A novel BRCA2 variant in the splice donor site of exon 21 (nucleotide 8982+1 G→A/c.8754+1 G→A) was identified. Exon trapping showed that the mutation activates a cryptic splice site 46 base pairs 3' of exon 21, resulting in the inclusion of a premature stop codon and synthesis of a truncated BRCA2 protein. The aberrant splicing was verified by RT-PCR analysis on RNA isolated from whole blood of the affected patient. The mutation was not found in any of the patient's parents or in the mother's carcinoma, showing it is a de novo mutation. Variant specific PCR indicates that the mutation arose in the male germ-line.
We conclude that the novel BRCA2 splice variant is a de novo mutation introduced in the male spermatozoa that can be classified as a disease causing mutation.
Germ-line BRCA2 (MIM# 600185) mutations in female carriers confer a lifetime risk exceeding 80% for breast cancer and 20% for ovarian cancer, and a moderate increased risk of other cancer types [1, 2]. Studies have indicated that the risk of ovarian cancer is greatest in women with BRCA2 mutations localized in nucleotides 3035–6629 [3, 4]. Several genes are likely to be involved in breast cancer predisposition, but no susceptibility gene aside BRCA2 and BRCA1 is likely to account for a large fraction or a major increase in risk .
The BRCA2 gene spans approximately 70 kb and is composed of 27 exons, which encodes a protein of 3418 amino acids. BRCA2 is involved in homologous recombination [6, 7], but is also suggested to play a role in transcriptional regulation  and cell cycle control . Mutations are distributed throughout the entire coding region of BRCA2 and to date numerous deleterious mutations have been reported (Breast Cancer Information Core [BIC]; ). The majority of patients with BRCA1 or BRCA2 associated breast and/or ovarian cancer have a family history, although healthy male carriers may obscure the apparent dominant trait. Several BRCA2 founder mutations have been identified, including the Ashkenazi Jewish nucleotide 6174delT mutation and the Icelandic nucleotide 999del5 mutation [11, 12]. In contrast, only two mutations in BRCA2 and one in BRCA1 have been reported as de novo mutations [13–15], but since a positive family history is one of the criteria for mutation screening, it is possible that we overlook a number of patients with these mutations.
Here we report the functional characterization of a novel de novo BRCA2 splice site mutation located in the intervening sequence (IVS) of exon 21 (nucleotide 8982+1 G→A/c.8754+1 G→A) in a Danish breast cancer patient with a family history of breast cancer. The parental origin of the mutation is assigned to the father.
Family B49363 is a 23 member, three-generation kindred with two affected subjects. A 40 year old woman was referred to genetic counseling two months after she had breast-conserving surgery at Roskilde County Hospital with radical excision of an 8 mm large invasive ductal carcinoma. The tumor was estrogen and progesterone receptor positive and had malignancy grade II. The patient received adjuvant radiotherapy followed by seven series of chemotherapy with CEF and tamoxifen. The patient's mother had a mastectomy at age 59 with excision of a 22 mm large estrogen and progesterone receptor positive invasive ductal carcinoma, malignancy grade II, and postoperatively she received tamoxifen. Following verbal and written consent, blood and tumour samples were collected from the proband for mutation screening and after renewed consent a third blood sample was collected for RNA analysis. Moreover, blood and tumour samples from the patient's mother, and blood samples from the patient's father and sister were collected. The family history was verified using the registry of the DBCG (Danish Breast Cancer Cooperative Group), hospital medical records and pathology reports, and genetic counselling was provided for each family member. Since the study is part of normal diagnostic procedures no ethical approval was obtained. The study was conducted in accordance with the Helsinki Declaration.
BRCA1 and BRCA2 screening
Genomic DNA was purified from whole blood using the QIAamp DNA mini kit (Qiagen) according to the manufacturer's instructions. BRCA1 and BRCA2 were amplified using intronic primer pairs flanking each exon. PCR products were pre-screened by dHPLC (denaturing high performance liquid chromatography) using the WAWE system (Transgenomic) and sequenced using an ABI3730 DNA analyzer (Applied Biosystems). Sequence variations were verified in a new blood sample. Moreover genomic DNA was examined by MLPA analysis (MRC-Holland). The BRCA1 variant is numbered according to GenBank accession number U14680, in which the A in the AUG start codon has number 120, whereas the BRCA2 variant is numbered according to GenBank accession number NC_000059, in which A in the AUG start codon has number 229. Moreover, the BRCA1 and BRCA2 variants are numbered according to GenBank accession number NC_000017.9 and NC_000013.9 using the guidelines from the Human Genetic Variation Society .
The pSPL3 vector was obtained from Gibco-BRL. pSPL3-BRCA2-wild-type and pSPL3-BRCA2-mutant plasmids, containing BRCA2 exon 21 and flanking intron sequences, were constructed by PCR using purified DNA from human blood samples and the following oligonucleotides: BRCA2-F, 5'-GATCACGAATTCTTCCTGGAAAACTTATAGCA-3' and BRCA2-R 5'-GATCACCTCGAGTTAGGGTAGAGGATTATCAAGTACA-3'. The PCR products were treated with EcoRI and XhoI and cloned into the pSPL3 vector. All constructs were verified by sequencing.
Cell culture and transfections
COS-7 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 4500 mg/l glucose supplemented with 10% foetal bovine serum, 100 U of penicillin per ml, and 100 μg of streptomycin per ml at 5% CO2 and 37°C. One day before transfection, cells were seeded in 6-well culture dishes at a density of 4 × 105 cells/well. Cells were transfected with a total of 4 μg plasmid DNA using FuGENE 6 transfection reagent (Roche) according to the manufacturer's instructions. Transfection efficiencies for each series were determined by cotransfection of pEGFP plasmid (Clontech). The next day the media was changed and after another 24 h the cells were harvested and total RNA was isolated using Trizol reagent (Invitrogen).
Exon trapping analysis
First strand cDNA was synthesized using 1 μg RNA, 20 μM SA2 primer (5'-ATCTCAGTGGTATTTGTGAGC-3') and M-MuLV reverse transcriptase (New England Biolabs). The cDNAs were amplified with pSPL3 vector-specific primers (5'-TCTGAGTCACCTGGACAACC-3' and 5'-ATCTCAGTGGTATTTGTGAGC-3') and the PCR products were resolved on a 2% agarose gel. All experiments were repeated three times.
A fresh blood sample was obtained from the patient. Total cellular RNA was isolated with Trizol (Invitrogen) according to the manufacturer's instructions. For reverse transcription-PCR (RT-PCR), cDNA was synthesized using the AMV reverse transcriptase (Promega) as described by the supplier. The cDNA were amplified with the BRCA2 specific primers 5'-CGGCCTGCTCGCTGGTAT-3' and 5'-GCCTTCCTAATTTCCAACTGGATCTG-3' resulting in a 503 bp fragment. The samples were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Finally, the bands were purified, cloned into pCR-Blunt II-TOPO (Invitrogen) and sequenced using an ABI3730 DNA analyzer (Applied Biosystems).
SNP chip analysis
Genomic DNA was applied to 250K StyI (~238.000 SNPs) SNP-microarray chips and processed according to the manufacturer's instructions (Affymetrix, UK). Briefly, 250 ng of genomic DNA was digested with StyI and ligated to adapters. Adapter-ligated DNA was amplified, purified, fragmented and labeled with biotin and hybridized to the arrays for 18 h. The Affymetrix 450 fluidics station and the Affymetrix 3000 G7 gene scanner were used to wash, stain, and scan the arrays. The CEL files were analysed using the BRLMM algorithm from Affymetrix Genotyping analysis software (GTYPE). Relationship was inferred by estimating the pairwise identical by descent (IBD) sharing, as described .
Assignment of parental origin
Variant specific primers were designed using the software Primer3 in combination with ClustaIW alignments. The primer sets distinguish the wild-type allele (BRCA2 c.8754+1-G forward: 5'-AGACCCAGCTTACCTTGACG-3') from the mutation carrying allele (BRCA2 c.8754+1-A forward: 5'-CAGACCCAGCTTACCTTGAAA-3'), and the two SNP alleles in rs4942485 (rs4942485*A reverse: 5'-CTACATTACAGATGGCTAATATCTGATT-3' and rs4942485*G reverse: 5'-CATTACAGATGGCTAATATCTGAAC-3'. Primer sets were tested on homozygote controls and unspecific annealing was avoided after introducing mismatches (marked in bold). PCR was carried out using standard conditions according to the manufacturer's protocols using the Expand High FidelityPLUS Taq DNA polymerase (Roche). Allele sizes were: BRCA2 c.8754+1-G and rs942485-G = 2297 bp, BRCA2 c.8754+1-*A and rs942485-G = 2298 bp, BRCA2 c.8754+1-G and rs942485-A = 2300 bp, BRCA2 c.8754+1-*A and rs942485-A = 2301 bp.
Results and discussion
To determine the parental origin of the mutation, variant specific PCR was performed using primers designed to distinguish between two SNP alleles and the mutation carrying allele from the wild-type allele. The father was homozygote for the haplotype GG, while the mother was homozygote for the haplotype GA (see Fig. 1). PCR analysis of the proband revealed a band using mutant BRCA2 and rs4942485*G primers, indicating that the haplotype *AG must derive from the father, and that the mutation therefore arose in the testicular germ cells changing BRCA2 nucleotide 8982+1/c.8754+1 from G to A. This is in agreement with previous findings in BRCA1 , whereas the studies describing de novo mutations in BRCA2 were unable to determine parental origin [13, 15]. Examinations of other cancer families, including MEN2B and retinoblastoma families have indicated that de novo mutations primarily occur in the male germ line [20, 21] and that the fathers age is a major determinant since mutations accumulate during life . It is, however, noteworthy that the age of the probands parents in our study was only 19 and 21 years at the time of birth of their affected daughter.
We conclude that the BRCA2 nucleotide 8982+1 G→A/c.8754+1 G→A mutation is a de novo mutation arising from the male germ line. The mutation is not previously reported in the BIC database. It leads to the activation of a cryptic splice site 46 base pairs 3' of exon 21 and introduces a premature stop codon and thereby a truncated BRCA2 protein. Therefore, this mutation can be classified as a disease-causing mutation. Analysis of intronic BRCA1 and BRCA2 variants by functional splicing assays can provide information that can be used clinically.
Stine Østergaard and Dorthe Petersen are acknowledged for technical assistance. Written consent was obtained from the patient or their relatives for publication of the study. This study was supported by the Neye Foundation.
- Easton DF, Ford D, Bishop DT: Breast and ovarian cancer incidence in BRCA1-mutation carriers. Breast Cancer Linkage Consortium. Am J Hum Genet. 1995, 56 (1): 265-271.PubMedPubMed CentralGoogle Scholar
- Wooster R, Neuhausen SL, Mangion J, Quirk Y, Ford D, Collins N, Nguyen K, Seal S, Tran T, Averill D, et al: Localization of a breast cancer susceptibility gene, BRCA2, to chromosome 13q12-13. Science. 1994, 265 (5181): 2088-2090. 10.1126/science.8091231.View ArticlePubMedGoogle Scholar
- Gayther SA, Mangion J, Russell P, Seal S, Barfoot R, Ponder BA, Stratton MR, Easton D: Variation of risks of breast and ovarian cancer associated with different germline mutations of the BRCA2 gene. Nat Genet. 1997, 15 (1): 103-105. 10.1038/ng0197-103.View ArticlePubMedGoogle Scholar
- Thompson D, Easton D: The genetic epidemiology of breast cancer genes. J Mammary Gland Biol Neoplasia. 2004, 9 (3): 221-236. 10.1023/B:JOMG.0000048770.90334.3b.View ArticlePubMedGoogle Scholar
- Smith P, McGuffog L, Easton DF, Mann GJ, Pupo GM, Newman B, Chenevix-Trench G, Szabo C, Southey M, Renard H, Odefrey F, Lynch H, Stoppa-Lyonnet D, Couch F, Hopper JL, Giles GG, McCredie MR, Buys S, Andrulis I, Senie R, Goldgar DE, Oldenburg R, Kroeze-Jansema K, Kraan J, Meijers-Heijboer H, Klijn JG, van Asperen C, van Leeuwen I, Vasen HF, Cornelisse CJ, Devilee P, Baskcomb L, Seal S, Barfoot R, Mangion J, Hall A, Edkins S, Rapley E, Wooster R, Chang-Claude J, Eccles D, Evans DG, Futreal PA, Nathanson KL, Weber BL, Rahman N, Stratton MR: A genome wide linkage search for breast cancer susceptibility genes. Genes Chromosomes Cancer. 2006, 45 (7): 646-655. 10.1002/gcc.20330.View ArticlePubMedPubMed CentralGoogle Scholar
- Davies AA, Masson JY, McIlwraith MJ, Stasiak AZ, Stasiak A, Venkitaraman AR, West SC: Role of BRCA2 in control of the RAD51 recombination and DNA repair protein. Mol Cell. 2001, 7 (2): 273-282. 10.1016/S1097-2765(01)00175-7.View ArticlePubMedGoogle Scholar
- Moynahan ME, Pierce AJ, Jasin M: BRCA2 is required for homology-directed repair of chromosomal breaks. Mol Cell. 2001, 7 (2): 263-272. 10.1016/S1097-2765(01)00174-5.View ArticlePubMedGoogle Scholar
- Shin S, Verma IM: BRCA2 cooperates with histone acetyltransferases in androgen receptor-mediated transcription. Proc Natl Acad Sci U S A. 2003, 100 (12): 7201-7206. 10.1073/pnas.1132020100.View ArticlePubMedPubMed CentralGoogle Scholar
- Marmorstein LY, Kinev AV, Chan GK, Bochar DA, Beniya H, Epstein JA, Yen TJ, Shiekhattar R: A human BRCA2 complex containing a structural DNA binding component influences cell cycle progression. Cell. 2001, 104 (2): 247-257. 10.1016/S0092-8674(01)00209-4.View ArticlePubMedGoogle Scholar
- Neuhausen S, Gilewski T, Norton L, Tran T, McGuire P, Swensen J, Hampel H, Borgen P, Brown K, Skolnick M, Shattuck-Eidens D, Jhanwar S, Goldgar D, Offit K: Recurrent BRCA2 6174delT mutations in Ashkenazi Jewish women affected by breast cancer. Nat Genet. 1996, 13 (1): 126-128. 10.1038/ng0596-126.View ArticlePubMedGoogle Scholar
- Thorlacius S, Olafsdottir G, Tryggvadottir L, Neuhausen S, Jonasson JG, Tavtigian SV, Tulinius H, Ogmundsdottir HM, Eyfjord JE: A single BRCA2 mutation in male and female breast cancer families from Iceland with varied cancer phenotypes. Nat Genet. 1996, 13 (1): 117-119. 10.1038/ng0596-117.View ArticlePubMedGoogle Scholar
- Robson M, Scheuer L, Nafa K, Ellis N, Offit K: Unique de novo mutation of BRCA2 in a woman with early onset breast cancer. J Med Genet. 2002, 39 (2): 126-128. 10.1136/jmg.39.2.126.View ArticlePubMedPubMed CentralGoogle Scholar
- Tesoriero A, Andersen C, Southey M, Somers G, McKay M, Armes J, McCredie M, Giles G, Hopper JL, Venter D: De novo BRCA1 mutation in a patient with breast cancer and an inherited BRCA2 mutation. Am J Hum Genet. 1999, 65 (2): 567-569. 10.1086/302503.View ArticlePubMedPubMed CentralGoogle Scholar
- van der Luijt RB, van Zon PH, Jansen RP, van der Sijs-Bos CJ, Warlam-Rodenhuis CC, Ausems MG: De novo recurrent germline mutation of the BRCA2 gene in a patient with early onset breast cancer. J Med Genet. 2001, 38 (2): 102-105. 10.1136/jmg.38.2.102.View ArticlePubMedPubMed CentralGoogle Scholar
- Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MA, Bender D, Maller J, Sklar P, de Bakker PI, Daly MJ, Sham PC: PLINK: A Tool Set for Whole-Genome Association and Population-Based Linkage Analyses. Am J Hum Genet. 2007, 81 (3): 559-575. 10.1086/519795.View ArticlePubMedPubMed CentralGoogle Scholar
- Hadjisavvas A, Charalambous E, Adamou A, Neuhausen SL, Christodoulou CG, Kyriacou K: Hereditary breast and ovarian cancer in Cyprus: identification of a founder BRCA2 mutation. Cancer Genet Cytogenet. 2004, 151 (2): 152-156. 10.1016/j.cancergencyto.2003.09.020.View ArticlePubMedGoogle Scholar
- Church DM, Stotler CJ, Rutter JL, Murrell JR, Trofatter JA, Buckler AJ: Isolation of genes from complex sources of mammalian genomic DNA using exon amplification. Nat Genet. 1994, 6 (1): 98-105. 10.1038/ng0194-98.View ArticlePubMedGoogle Scholar
- Carlson KM, Bracamontes J, Jackson CE, Clark R, Lacroix A, Wells SA, Goodfellow PJ: Parent-of-origin effects in multiple endocrine neoplasia type 2B. Am J Hum Genet. 1994, 55 (6): 1076-1082.PubMedPubMed CentralGoogle Scholar
- Kato MV, Ishizaki K, Shimizu T, Ejima Y, Tanooka H, Takayama J, Kaneko A, Toguchida J, Sasaki MS: Parental origin of germ-line and somatic mutations in the retinoblastoma gene. Hum Genet. 1994, 94 (1): 31-38. 10.1007/BF02272838.View ArticlePubMedGoogle Scholar
- Chandley AC: On the parental origin of de novo mutation in man. J Med Genet. 1991, 28 (4): 217-223.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2350/9/58/prepub
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