Figure 2
From: Novel de novo BRCA2mutation in a patient with a family history of breast cancer
Identification of the BRCA2 nucleotide 8982+1 G→A/c.8754+1 G→A variant. DNA was purified from whole blood and BRCA2 exon 21 was amplified using the primers 5'-CTTTGGGTGTTTTATGCTTGT-3' and 5'-CTGGCACATCACTGAAAATC-3' and sequenced. The analysis revealed a nucleotide 8982+1 G→A/c.8754+1 G→A mutation in BRCA2 (sense strand). The nucleotide 8982+1 G→A/c.8754+1 G→A mutation and the cryptic splice site are underlined.