- Research article
- Open Access
- Open Peer Review
Association study of functional genetic variants of innate immunity related genes in celiac disease
© Rueda et al; licensee BioMed Central Ltd. 2005
- Received: 29 March 2005
- Accepted: 03 August 2005
- Published: 03 August 2005
Recent evidence suggest that the innate immune system is implicated in the early events of celiac disease (CD) pathogenesis. In this work for the first time we have assessed the relevance of different proinflammatory mediators typically related to innate immunity in CD predisposition.
We performed a familial study in which 105 celiac families characterized by the presence of an affected child with CD were genotyped for functional polymorphisms located at regulatory regions of IL-1α, IL-1β, IL-1RN, IL-18, RANTES and MCP-1 genes. Familial data was analysed with a transmission disequilibrium test (TDT) that revealed no statistically significant differences in the transmission pattern of the different genetic markers considered.
The TDT analysis for IL-1α, IL-1β, IL-1RN, IL-18, and MCP-1 genes genetic variants did not reveal biased transmission to the affected offspring. Only a borderline association of RANTES promoter genetic variants with CD predisposition was observed.
Our results suggest that the analysed polymorphisms of IL-1α, IL-1β, IL-1RN, IL-18, RANTES and MCP-1 genes do not seem to play a major role in CD genetic predisposition in our population.
- Celiac Disease
- Celiac Disease Patient
- Transmission Disequilibrium Test
- Gluten Peptide
- Central Nervous System Lupus
Celiac disease (CD) is an autoimmune disorder of the small intestine in which dietary gluten ingestion leads to a chronic inflammatory status of the mucosa . There is strong evidence for a genetic component for CD, with the HLA genes being the strongest genetic locus associated with CD predisposition known to date. About 95% of CD patients are carriers of the DQ2 molecule, encoded by DQA1*05/DQB1*02 alleles, compared to ~10% of healthy control subjects. Furthermore, the DQ8 molecule (DQA1*0301/DQB1*0302 is also found more frequently in CD patients although to a lesser extent . Finally, a role for genes located outside the HLA region has been suggested since the overall contribution of HLA genes to CD genetic predisposition is no more than 40% .
T CD4+ lymphocytes are key elements in the induction and progression of CD pathogenesis. Certain gluten peptides bound to DQ2 or DQ8 molecules cause proliferation and production of proinflammatory cytokines by lamina propria CD4 +T cells . Besides this activation of adaptive immune response, recent evidences suggest that there is an implication of the innate immunity in the initial phases of CD . In this regard, some gluten peptides have been demonstrated to drive a danger signal that leads to an activation of the innate immune system [5, 6] and additionally it is thought that bacteria may play a role in CD . In fact, CD patients show an up-regulation in the expression of pro-inflammatory cytokines typically related to the innate immune response, such us IL1, IL-18 and chemokines [6, 8–10].
The IL1 gene cluster located in the chromosomal region 2q12-22 codifies for three proteins: IL-1α, IL-1β and IL-1 receptor agonist (IL-1RN), of which the two first are strong inducers of inflammation while IL-1RN is an effective antagonist binding to the IL-1 receptor without activating the target cell . These genes are polymorphic bearing well-characterized single nucleotide polymorphisms (SNPs). Polymorphisms in IL-1α at position -889 C/T (rs1800587) and IL-1β at position -511 C/T (rs1143627) were described [12, 13]. Furthermore, recent findings showed that the -511 C/T IL-1β genetic variant is related to differences in IL-1β protein secretion . The IL-1RN gene contains within its second intron a variable number of an 86-bp tandem repeats (rs380092) , showing the allele 2 (IL-1RN*2; two repeats) an increased frequency in a variety of autoimmune and inflammatory disorders .
Another important member of the proinflammatory IL-1 family is IL-18, which is thought to be a key regulator of cytokine expression . Furthermore, a role for IL-18 in the induction of an anti-gluten inflammatory response has been suggested [10, 18, 19]. It is thought that IL-18 gene variation in the promoter region regulates the expression of this cytokine . Interestingly, in the IL-18 promoter region two SNPs -607 A/C (rs1946518) and -137 G/C (rs187238) were described, which are supposed to alter the IL-18 promoter activity .
Moreover, raised levels of chemokines such us RANTES (regulated upon activation, normal T-cells expressed and secreted) and monocyte chemoatractant protein-1 (MCP-1) have been observed in the primary immune response to gluten in CD patients [6, 8]. Interestingly, genetic variants within regulatory regions that can affect trancription and protein production levels, RANTES -403 G/A (rs2107538) and -28 G/C (rs2280788) and MCP-1 -2518 G/A (rs1024611) SNPs, were described [22–24].
Taking into consideration these findings, in this work we aimed to investigate the possible implication of IL-1α, IL-1β, IL-1RN, IL-18, RANTES and MCP-1 functional polymorphisms in CD susceptibility.
In the present work we have analysed a panel of 105 celiac families characterised by the presence of an affected child with CD. The study participants were recruited at "Hospital Materno-Infantil" and "Hospital Clinico Universitario", Granda, (Spain) and were of Spanish Caucasian origin. All patients were diagnosed following the European Society of Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) criteria for CD . Their age at study was 7.1 ± 3.9 years and the mean age for disease diagnosis was 2.7 ± 2.72. A 60% were women and 40 % men, showing an anthropometry at diagnosis (weight and height) of P 3–100 percentile. The mean age of gluten introduction was 6.4 ± 1.5 months. Typical symptoms were observed in 72.2 % of patients and 27.8 % showed atypical symptoms. All family members were genotyped in DRB1 and DQB1. DQA1 typing was deduced from DQB1 and DRB1 typing on the basis of the strong linkage disequilibrium among HLA class II alleles.
Taqman probes used for cytokine genotyping
Taqman probe sequence
IL-1A -889 C/T (rs1800587)
VIC – CCTTCAATGGTGTTGCC
FAM – CCTTCAATGATGTTGCC
IL-1B -551 C/T (rs1143627)
VIC – CTGTTTTTATGGCTTTCA
FAM – CTGTTTTTATAGCTTTCA
IL-18 -607 C/A (rs1946518)
VIC – ATCATTAGAATTTTATGTAATAAT
FAM – ATCATTAGAATTTTATTTAATAAT
IL-18 -137 G/C (rs187238)
VIC – ACTATTTTCATGAAATCTTTTCT
FAM – TTTTCATGAAATGTTTTCT
RANTES -28 G/C (rs2280788)
VIC – CCCCTCAACTGGC
FAM – CCCCTGAACTGGC
MCP-1 -2518 G/A (rs1024611)
VIC – CAGACAGCTGTCACTTT
FAM – CAGACAGCTATCACTTT
The IL-1RN polymorphism was genotyped by PCR as previously described . Briefly, we used a froward primer 5'- CTC AGC AAC ACCT CCT AT and reverse primer 5'- TCC TGG TCT GCA GGT AA, two amplify five possible alleles with different PCR fragment size: 410 bp (allele 1: 4 repeats), 240 bp (allele 2: two repeats), 325 bp (allele 3: 3 repeats), 500 bp (allele 4: 5 repeats), and 595 bp (allele 5: 6 repeats).
We used the UNPHASED software created for TDT and case-control analysis . We performed a Transmission Disequilibrium Test (TDT), which assesses allele transmission rates in simplex families and tests for deviation from expected 50% transmission. For the haplotype analysis, pair-wise linkage disequilibrium measures were investigated and haplotypes constructed using the expectation-maximization (EM) algorithm implemented in UNPHASED software. The power of the study to detect an effect of a polymorphism in disease susceptibility was estimated using the Quanto v 0.5 software (Department of Preventive Medicine University of Southern California, California, USA) .
IL1 gene cluster
Allelic frequencies and percentage of transmission of IL-1α, IL-1β, IL-1RN, IL-18, RANTES and MCP-1 genetic variants in CD families.
Alelle frequency in parents (%)
The TDT analysis for -607 A/C and -137 G/C IL-18 promoter genetic variants did not reveal biased transmission of any of the alleles to the affected offspring (Table 2).
Transmission pattern of haplotypes inferred for IL-18 and RANTES promoter genetic variant in CD families
No transmitted (%)
MCP-1 and RANTES
After analyzing the MCP-1 -2518 G/A alleles transmission we observed that none of the alleles was preferentially transmitted from heterozygous parents to the affected offspring (Table 2). Regarding to the RANTES promoter genetic variants the mutant alleles -403 A and -28 G showed an overall allele frequency similar to that expected for Caucasian populations (84.1% and 96.5% respectively in our population) [29, 30]. The transmission of both -403G/A and -28 C/G SNPs showed a slightly deviation from the 50% expected transmission pattern (Table 2). Alleles -403 G and -28 C were more transmitted to the affected offspring with borderline significance (P = 0.04 and P = 0.06 respectively) (Table 2). In addition, we estimated haplotypes for both genetic variants. Three out of the four haplotypic combinations were observed, being the -403G/-28C and -403A/-28C haplotypes the most common in CD families. No significant distorted transmission pattern for RANTES promoter haplotypes was observed (Table 3).
CD is considered a model for autoimmune disorders since many of the components that generate the altered immune response to gluten have been well characterized . However, there are some relevant events of CD pathogenesis that remain unclear, for instance the stimuli that drives the high IFNγ levels in the small intestine of CD patients and why only one out of 20–30 DQ2-positive individuals develops CD . An explanation for these questions might be provided from recent studies that point out a role for the innate immunity in CD . This finding supports a novel focus of research in CD molecular and genetic basis, opening a new field for the functional search of CD candidate genes.
In this work, for the first time we have assessed the relevance of IL-1α, IL-1β, IL-1RN, IL-18, RANTES and MCP-1 genes in CD predisposition. All these genes have been previously associated with susceptibility to several autoimmune disorders [31–40]. However, we failed to detect an association of IL-1α, IL-1β, IL-1RN, IL-18, and MCP-1 genes with CD predisposition using a TDT analysis in our cohort of 105 simplex CD families. Only a borderline significant association of RANTES promoter genetic variants with CD predisposition was observed.
Several studies have focused on the role of RANTES -403G/A and -28 G/C promoter polymorphisms in susceptibility to different autoimmune disorders. The RANTES -403A allele has been associated with susceptibility to multiple sclerosis (MS) and polymialgia rheumatica [41, 42]. On the other hand, the RANTES -28G allele was observed to be a genetic risk for clinical complications such us diabetic neprhopathy, early onset of MS, lower levels of C3 in SLE, and higher incidence of central nervous system lupus [37, 38, 41]. Both RANTES -403A and -28G alleles were associated with higher RANTES expression levels [22, 23]. However, considering the multiple testing of the 6 different genes of our study, the association observed for RANTES promoter variants in our population can not be considered as being significant. Therefore, our results of RANTES suggest that further studies should be performed to clarify the role of RANTES in CD and autoimmune diseases in general.
Using a familial approach we eliminate the risk of population stratification derived from case-control association studies. In addition, we estimated that our study design would have considerable power to detect the effect of a polymorphism with moderate to high risk for CD. Assuming an additive model, a minor allele frequency of 0.30 (corresponding to a median value of the majority of markers considered) and RR of 1.8 we would reach 81% power to detect an association in our population. Nevertheless, under a dominant model the power drops to 49% and considering a lower disease allele frequency, for instance 0.16 as is the case of RANTES -28, our study power would decrease to a 64% for a RR of 1.8, and increases to 82% when we assume a RR of 2.0. For this reason, the low level of significance that our TDT analysis reached for RANTES promoter genetic variants might well reflect a true positive, and therefore needs further confirmation using a larger group of CD families.
Taking into account our findings, it is suggested that the analysed genetic polymorphisms of IL-1α, IL1-β, IL-1RN, IL-18, RANTES and MCP-1 genes seem not to play a major role in CD susceptibility in our population. It might be possible that the release of these cytokines and chemokines observed in CD patients could be derived from the activity of other innate immunity related pro-inflammatory mediators with higher influence in disease pathogenesis. In this regard, it is known that in CD the cytokine expression pattern in response to gluten is strongly dominated by IFNγ . Of note, in a recent work we assessed the influence of a functional dinucleotide polymorphism of IFNγ gene in CD predisposition. An association of a higher IFNγ producer allele with CD was observed, supporting a possible explanation for the high levels of INFγ observed in intestinal mucosa of CD patients .
Other proinflammatory mediators related with innate immunity such as, TNF-α and IL-12, has been analysed with respect to CD susceptibility. In accordance with our findings no evidence of association was found between IL-12 and CD in two independent studies [45, 46]. Regarding TNF-α it has been difficult to dissect the relevance of this genetic marker in CD since it maps within HLA clas III region and it shows linkage disequilibrium with CD disease predisposing DQ2 alleles. In fact controversial results have been obtained, and there is no consensus about an independent or due to linkage disequilibrium role of TNF-α in CD susceptibility [47, 48].
Our results suggest that IL-1α, IL1-β, IL-1RN, IL-18, RANTES and MCP-1 genetic variants do not play a major role in CD genetic predisposition, although the suggestive evidence for RANTES deserves further investigation. Furthermore, we consider the innate response an intriguing focus of research and it should be of interest to investigate the role of other cytokines up-regulated in the early events of CD.
This work was supported by grant SAF03-460 from Plan Nacional de I+D (CICYT), and in part by Consejería de Educación, Junta de Andalucía, grupo CTS-180.
B.K. is supported by the Dutch Diabetes Research Foundation, The Netherlands Organisation for Health Research and Development (ZonMW) and The Juvenile Diabetes Research Foundation International (JDRF) (2001.10.004).
- Sollid LM: Coeliac disease: dissecting a complex inflammatory disorder. Nature Review Immunology. 2002, 2: 647-10.1038/nri885.View ArticleGoogle Scholar
- Louka AS, Sollid LM: HLA in coeliac disease: Unravelling the complex genetics of a complex disorder. Tissue Antigens. 2003, 61: 105-117.View ArticlePubMedGoogle Scholar
- Green PH, Jabri B: Coeliac disease. Lancet. 2003, 362: 383-391. 10.1016/S0140-6736(03)14027-5.View ArticlePubMedGoogle Scholar
- Schuppan D, Esslinger B, Dieterich W: Innate immunity and coeliac disease. Lancet. 2003, 362: 3-4. 10.1016/S0140-6736(03)13843-3.View ArticlePubMedGoogle Scholar
- Maiuri L, Ciacci C, Ricciardelli I, Vacca L, Raia V, Auricchio S, Picard J, Osman M, Quaratino S, Londei M: Association between innate response to gliadin and activation of pathogenic T cells in coeliac disease. Lancet. 2003, 362: 30-37. 10.1016/S0140-6736(03)13803-2.View ArticlePubMedGoogle Scholar
- Tuckova L, Novotna J, Novak P, Flegelova Z, Kveton T, Jelinkova L, Zidek Z, Man P, Tlaskalova-Hogenova H: Activation of macrophages by gliadin fragments: isolation and characterization of active peptide. J Leukoc Biol. 2002, 71: 625-631.PubMedGoogle Scholar
- Forsberg G, Fahlgren A, Horstedt P, Hammarstrom S, Hernell O, Hammarstrom ML: Presence of bacteria and innate immunity of intestinal epithelium in childhood celiac disease. Am J Gastroenterol. 2004, 99: 894-904. 10.1111/j.1572-0241.2004.04157.x.View ArticlePubMedGoogle Scholar
- Chowers Y, Marsh MN, De Grandpre L, Nyberg A, Theofilopoulos AN, Kagnoff MF: Increased proinflammatory cytokine gene expression in the colonic mucosa of coeliac disease patients in the early period after gluten challenge. Clin Exp Immunol. 1997, 107: 141-147. 10.1046/j.1365-2249.1997.d01-892.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Fornari MC, Pedreira S, Niveloni S, Gonzalez D, Diez RA, Vazquez H, Mazure R, Sugai E, Smecuol E, Boerr L, et al: Pre- and post-treatment serum levels of cytokines IL-1beta, IL-6, and IL-1 receptor antagonist in celiac disease. Are they related to the associated osteopenia?. Am J Gastroenterol. 1998, 93: 413-418.View ArticlePubMedGoogle Scholar
- Salvati VM, MacDonald TT, Bajaj-Elliott M, Borrelli M, Staiano A, Auricchio S, Troncone R, Monteleone G: Interleukin 18 and associated markers of T helper cell type 1 activity in coeliac disease. Gut. 2002, 50: 186-190. 10.1136/gut.50.2.186.View ArticlePubMedPubMed CentralGoogle Scholar
- Arend WP: The balance between IL-1 and IL-1Ra in disease. Cytokine Growth Factor Rev. 2002, 13: 323-340. 10.1016/S1359-6101(02)00020-5.View ArticlePubMedGoogle Scholar
- di Giovine FS, Takhsh E, Blakemore AI, Duff GW: Single base polymorphism at -511 in the human interleukin-1 beta gene (IL1 beta). Hum Mol Genet. 1992, 1: 450-View ArticlePubMedGoogle Scholar
- McDowell TL, Symons JA, Ploski R, Forre O, Duff GW: A genetic association between juvenile rheumatoid arthritis and a novel interleukin-1 alpha polymorphism. Arthritis Rheum. 1995, 38: 221-228.View ArticlePubMedGoogle Scholar
- Hall SK, Perregaux DG, Gabel CA, Woodworth T, Durham LK, Huizinga TW, Breedveld FC, Seymour AB: Correlation of polymorphic variation in the promoter region of the interleukin-1 beta gene with secretion of interleukin-1 beta protein. Arthritis Rheum. 2004, 50: 1976-1983. 10.1002/art.20310.View ArticlePubMedGoogle Scholar
- Tarlow JK, Blakemore AI, Lennard A, Solari R, Hughes HN, Steinkasserer A, Duff GW: Polymorphism in human IL-1 receptor antagonist gene intron 2 is caused by variable numbers of an 86-bp tandem repeat. Hum Genet. 1993, 91: 403-404. 10.1007/BF00217368.View ArticlePubMedGoogle Scholar
- Witkin SS, Gerber S, Ledger WJ: Influence of interleukin-1 receptor antagonist gene polymorphism on disease. Clin Infect Dis. 2002, 34: 204-209. 10.1086/338261.View ArticlePubMedGoogle Scholar
- Gracie JA, Robertson SE, McInnes IB: Interleukin-18. J Leukoc Biol. 2003, 73: 213-224. 10.1189/jlb.0602313.View ArticlePubMedGoogle Scholar
- Eaton AD, Xu D, Garside P: Administration of exogenous interleukin-18 and interleukin-12 prevents the induction of oral tolerance. Immunology. 2003, 108: 196-203. 10.1046/j.1365-2567.2003.01570.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Lettesjo H, Hansson T, Bergqvist A, Gronlund J, Dannaeus A: Enhanced interleukin-18 levels in the peripheral blood of children with coeliac disease. Clin Exp Immunol. 2005, 139: 138-143. 10.1111/j.1365-2249.2005.02661.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Tone M, Thompson SA, Tone Y, Fairchild PJ, Waldmann H: Regulation of IL-18 (IFN-gamma-inducing factor) gene expression. J Immunol. 1997, 159: 6156-6163.PubMedGoogle Scholar
- Giedraitis V, He B, Huang WX, Hillert J: Cloning and mutation analysis of the human IL-18 promoter: a possible role of polymorphisms in expression regulation. J Neuroimmunol. 2001, 112: 146-152. 10.1016/S0165-5728(00)00407-0.View ArticlePubMedGoogle Scholar
- Liu H, Chao D, Nakayama EE, Taguchi H, Goto M, Xin X, Takamatsu JK, Saito H, Ishikawa Y, Akaza T, et al: Polymorphism in RANTES chemokine promoter affects HIV-1 disease progression. Proc Natl Acad Sci U S A. 1999, 96: 4581-4585. 10.1073/pnas.96.8.4581.View ArticlePubMedPubMed CentralGoogle Scholar
- McDermott DH, Beecroft MJ, Kleeberger CA, Al-Sharif FM, Ollier WE, Zimmerman PA, Boatin BA, Leitman SF, Detels R, Hajeer AH, et al: Chemokine RANTES promoter polymorphism affects risk of both HIV infection and disease progression in the Multicenter AIDS Cohort Study. Aids. 2000, 14: 2671-2678. 10.1097/00002030-200012010-00006.View ArticlePubMedGoogle Scholar
- Rovin BH, Lu L, Saxena R: A novel polymorphism in the MCP-1 gene regulatory region that influences MCP-1 expression. Biochem Biophys Res Commun. 1999, 259: 344-348. 10.1006/bbrc.1999.0796.View ArticlePubMedGoogle Scholar
- Revised criteria for diagnosis of coeliac disease. Report of Working Group of European Society of Paediatric Gastroenterology and Nutrition. Arch Dis Child. 1990, 65: 909-11.Google Scholar
- Craggs A, West S, Curtis A, Welfare M, Hudson M, Donaldson P, Mansfield J: Absence of a genetic association between IL-1RN and IL-1B gene polymorphisms in ulcerative colitis and Crohn disease in multiple populations from northeast England. Scand J Gastroenterol. 2001, 36: 1173-1178. 10.1080/00365520152584806.View ArticlePubMedGoogle Scholar
- Dudbridge F: Methods and software for association tests of uncertain haplotypes in case-parent trios. Am J Hum Genet. 2002, 71 (Suppl): A2338-[http://portal.litbio.org/Registered/Webapp/glue/]Google Scholar
- Gauderman WJ: Candidate gene association analysis for a quantitative trait, using parent-offspring trios. Genet Epidemiol. 2003, 25: 327-338. 10.1002/gepi.10262.View ArticlePubMedGoogle Scholar
- al Sharif F, Ollier WE, Hajeer AH: A rare polymorphism at position -28 in the human RANTES promoter. Eur J Immunogenet. 1999, 26: 373-374. 10.1046/j.1365-2370.1999.00162.x.View ArticlePubMedGoogle Scholar
- Hajeer AH, al Sharif F, Ollier WE: A polymorphism at position -403 in the human RANTES promoter. Eur J Immunogenet. 1999, 26: 375-376. 10.1046/j.1365-2370.1999.00163.x.View ArticlePubMedGoogle Scholar
- Parks CG, Cooper GS, Dooley MA, Treadwell EL, St Clair EW, Gilkeson GS, Pandey JP: Systemic lupus erythematosus and genetic variation in the interleukin 1 gene cluster: a population based study in the southeastern United States. Ann Rheum Dis. 2004, 63: 91-94. 10.1136/ard.2003.007336.View ArticlePubMedPubMed CentralGoogle Scholar
- Timms AE, Crane AM, Sims AM, Cordell HJ, Bradbury LA, Abbott A, Coyne MR, Beynon O, Herzberg I, Duff GW, et al: The interleukin 1 gene cluster contains a major susceptibility locus for ankylosing spondylitis. Am J Hum Genet. 2004, 75: 587-595. 10.1086/424695.View ArticlePubMedPubMed CentralGoogle Scholar
- Mann CL, Davies MB, Stevenson VL, Leary SM, Boggild MD, Ko Ko C, Jones PW, Fryer AA, Strange RC, Thompson AJ, et al: Interleukin 1 genotypes in multiple sclerosis and relationship to disease severity. J Neuroimmunol. 2002, 129: 197-204. 10.1016/S0165-5728(02)00181-9.View ArticlePubMedGoogle Scholar
- Kaijzel EL, van Dongen H, Bakker AM, Breedveld FC, Huizinga TW, Verweij CL: Relationship of polymorphisms of the Interleukin-1 gene cluster to occurrence and severity of rheumatoid arthritis. Tissue Antigens. 2002, 59: 122-126. 10.1034/j.1399-0039.2002.590208.x.View ArticlePubMedGoogle Scholar
- Ide A, Kawasaki E, Abiru N, Sun F, Fukushima T, Ishii R, Takahashi R, Kuwahara H, Fujita N, Kita A, et al: Association of interleukin-18 gene promoter polymorphisms in type 1 diabetes and autoimmune thyroid disease. Ann N Y Acad Sci. 2003, 1005: 436-439. 10.1196/annals.1288.074.View ArticlePubMedGoogle Scholar
- Novota P, Kolostova K, Pinterova D, Novak J, Treslova L, Andel M, Cerna M: Interleukin IL-18 gene promoter polymorphisms in adult patients with type 1 diabetes mellitus and latent autoimmune diabetes in adults. Immunol Lett. 2005, 96: 247-251. 10.1016/j.imlet.2004.08.016.View ArticlePubMedGoogle Scholar
- Nakajima K, Tanaka Y, Nomiyama T, Ogihara T, Ikeda F, Kanno R, Iwashita N, Sakai K, Watada H, Onuma T, et al: RANTES promoter genotype is associated with diabetic nephropathy in type 2 diabetic subjects. Diabetes Care. 2003, 26: 892-898.View ArticlePubMedGoogle Scholar
- Liao CH, Yao TC, Chung HT, See LC, Kuo ML, Huang JL: Polymorphisms in the promoter region of RANTES and the regulatory region of monocyte chemoattractant protein-1 among Chinese children with systemic lupus erythematosus. J Rheumatol. 2004, 31: 2062-2067.PubMedGoogle Scholar
- Tucci M, Barnes EV, Sobel ES, Croker BP, Segal MS, Reeves WH, Richards HB: Strong association of a functional polymorphism in the monocyte chemoattractant protein 1 promoter gene with lupus nephritis. Arthritis Rheum. 2004, 50: 1842-1849. 10.1002/art.20266.View ArticlePubMedGoogle Scholar
- Kim HL, Lee DS, Yang SH, Lim CS, Chung JH, Kim S, Lee JS, Kim YS: The polymorphism of monocyte chemoattractant protein-1 is associated with the renal disease of SLE. Am J Kidney Dis. 2002, 40: 1146-1152. 10.1053/ajkd.2002.36858.View ArticlePubMedGoogle Scholar
- Gade-Andavolu R, Comings DE, MacMurray J, Vuthoori RK, Tourtellotte WW, Nagra RM, Cone LA: RANTES: a genetic risk marker for multiple sclerosis. Mult Scler. 2004, 10: 536-539. 10.1191/1352458504ms1080oa.View ArticlePubMedGoogle Scholar
- Makki RF, al Sharif F, Gonzalez-Gay MA, Garcia-Porrua C, Ollier WE, Hajeer AH: RANTES gene polymorphism in polymyalgia rheumatica, giant cell arteritis and rheumatoid arthritis. Clin Exp Rheumatol. 2000, 18: 391-393.PubMedGoogle Scholar
- Nilsen EM, Jahnsen FL, Lundin KE, Johansen FE, Fausa O, Sollid LM, Jahnsen J, Scott H, Brandtzaeg P: Gluten induces an intestinal cytokine response strongly dominated by interferon gamma in patients with celiac disease. Gastroenterology. 1998, 115: 551-563.View ArticlePubMedGoogle Scholar
- Rueda B, Martinez A, Lopez-Nevot MA, Mas-Fontao A, Paco L, Ortega E, Fernandez-Arquero M, Urcelay E, Gomez de la Concha E, Martin J: A functional variant of IFNgamma gene is associated with coeliac disease. Genes Immun. 2004, 5: 517-519. 10.1038/sj.gene.6364115.View ArticlePubMedGoogle Scholar
- Louka AS, Torinsson Naluai A, D'Alfonso S, Ascher H, Coto I, Ek J, Giordano M, Gudjonsdottir AH, Mellai M, Momigliano-Richiardi P, et al: The IL12B gene does not confer susceptibility to coeliac disease. Tissue Antigens. 2002, 59: 70-72. 10.1034/j.1399-0039.2002.590117.x.View ArticlePubMedGoogle Scholar
- Seegers D, Borm ME, van Belzen MJ, Mulder CJ, Bailing J, Crusius JB, Meijer JW, Wijmenga C, Pena AS, Bouma G: IL12B and IRF1 gene polymorphisms and susceptibility to celiac disease. Eur J Immunogenet. 2003, 30: 421-425. 10.1111/j.1365-2370.2003.00428.x.View ArticlePubMedGoogle Scholar
- de la Concha EG, Fernandez-Arquero M, Vigil P, Rubio A, Maluenda C, Polanco I, Fernandez C, Figueredo MA: Celiac disease and TNF promoter polymorphisms. Hum Immunol. 2000, 61: 513-517. 10.1016/S0198-8859(99)00187-1.View ArticlePubMedGoogle Scholar
- Woolley N, Mustalahti K, Maki M, Partanen J: Cytokine gene polymorphisms and genetic association with coeliac disease in the Finnish population. Scand J Immunol. 2005, 61: 51-56. 10.1111/j.0300-9475.2005.01525.x.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2350/6/29/prepub
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