The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer
© Chen et al; licensee BioMed Central Ltd. 2011
Received: 11 September 2010
Accepted: 13 January 2011
Published: 13 January 2011
Qualitative and quantitative changes in human mitochondrial DNA (mtDNA) have been implicated in various cancer types. A 4,977 bp deletion in the major arch of the mitochondrial genome is one of the most common mutations associated with a variety of human diseases and aging.
We conducted a comprehensive study on clinical features and mtDNA of 104 colorectal cancer patients in the Wenzhou area of China. In particular, using a quantitative real time PCR method, we analyzed the 4,977 bp deletion and mtDNA content in tumor tissues and paired non-tumor areas from these patients.
We found that the 4,977 bp deletion was more likely to be present in patients of younger age (≤65 years, p = 0.027). In patients with the 4,977 bp deletion, the deletion level decreased as the cancer stage advanced (p = 0.031). Moreover, mtDNA copy number in tumor tissues of patients with this deletion increased, both compared with that in adjacent non-tumor tissues and with in tumors of patients without the deletion. Such mtDNA content increase correlated with the levels of the 4,977 bp deletion and with cancer stage (p < 0.001).
Our study indicates that the mtDNA 4,977 bp deletion may play a role in the early stage of colorectal cancer, and it is also implicated in alteration of mtDNA content in cancer cells.
Colorectal cancer is one of the leading human malignancies . While its morbidity and mortality have declined in western countries in recent years, both the incidence and deaths caused by this cancer have increased significantly in recent years in Asia , particularly in China . Both genetic and environmental factors contribute to colorectal cancer development. Based on a study on cohorts of twins from Sweden, Denmark and Finland, heritable factors contributed about 35% to colorectal cancer , while in another nationwide family study conducted with 9.6 million Swedish people, around 13% of colorectal susceptibility was attributed to genetic effects . However, up to now, only 6% of colorectal cancer can be ascribed to mutations in particular genes . Among those genes associated with predispositions for colorectal cancer are adenomatous polyposis coli (APC), a tumor suppressor involving cell adhesion, signal transduction and transcription activation, and DNA mismatch repair (MMR) genes [6, 7].
Mitochondria, known as the cellular power plants, also regulate cell death and cell proliferation [8, 9]. Defects in mitochondrial function have long been hypothesized to play a role in tumorigenesis . Mitochondria possess their own genomes. Human mtDNA encodes 13 essential subunits of the oxidative phosphorylation (OXPHOS) system as well as 2 rRNAs and 22 tRNAs used for mitochondrial translation . Alterations in mtDNA both qualitatively (mutations) [12–14] and quantitatively (mtDNA copy number)  have been associated with many human diseases including neurodegenerative diseases, metabolic diseases and various types of cancer [16–18].
The mtDNA is subject to relatively high oxidative damage and at the same time is sensitive to such damage. It has also been shown that oxidative modified DNA is especially prone to mispairing of repetitive elements and is correlated with deletions . In fact, large scale deletions were among the first mtDNA mutations identified to cause human diseases [20, 21]. Up to now, more than 100 deletions have been reported to be associated with various diseases (http://www.mitomap.org/). Among these deletions, a 4,977-bp deletion occurring between two 13-bp direct repeats at positions 13447-13459 and 8470-8482 has attracted tremendous interests since it is the common cause of several sporadic diseases including Pearson's syndrome, Kearns-Sayre syndrome (KSS) and chronic progressive external ophthalmoplegia (CPEO) [13, 22], and is therefore called the "common" deletion. This deletion also accumulates in many tissues during aging, and has been used as an mtDNA damage biomarker [18, 23].
mtDNA mutations, including both point mutations and deletions, have been identified in various types of human cancer [16, 17, 24]. In one of the first comprehensive studies of mtDNA in cancer cells, it was demonstrated that among ten colorectal cancer cell lines, seven of them exhibited mtDNA mutations . In another study focused on the major control region of mtDNA, the "displacement loop" (D-loop) in French colorectal cancer patients, the presence of tumor D-loop mutations correlated with poor prognosis .
To address the question if the mtDNA 4,977-bp deletion plays a role in pathogenesis and development of colorectal cancer, we studied 104 colorectal cancer patients recently admitted in the First Affiliated Hospital of Wenzhou Medical College and carried out a systematic investigation into their clinicopathological features and characterized their mtDNA, in particular the 4,977-bp deletion and the mtDNA content, in the tumor tissues and nearby non-tumor areas.
Paraffin-embedded tumor tissues and paired adjacent non-tumor tissues were collected from 104 unrelated colorectal cancer patients prior to any chemotherapy, radiotherapy or pharmacotherapy at the First Affiliated Hospital of Wenzhou Medical College between October, 2006 and March, 2008. Informed consent from all patients in this study was obtained under protocols approved by the Wenzhou Medical College Ethics Committee. The patients ranged in age from 33 to 87 years (mean ± SD, 65.58 ± 11.49), and were classified according to the tumor-node-metastasis (TNM) staging system (American Joint Committee on Cancer): 15 were at stage I, 45 at stage II, 39 at stage III and 5 at stage IV. Ten-micron sections were cut from paraffin blocks and classifications were confirmed by a senior pathologist using a standard hematoxylin & eosin staining protocol.
Detection of the mtDNA 4,977-bp deletion
Determination of mtDNA content and levels of the 4,977 bp deletion
The mtDNA content was measured by a real-time PCR on cytochrome c oxidase I (COX I) gene and normalized by simultaneous measurement of nuclear DNA encoded β -actin genes. QPCR was carried out using an ABI 7900HT Fast Real-Time PCR System (Applied Biosystems) in a 20 μl reaction in different tubes containing 0.5 μM each of the forward and reverse primers, 0.1 pM for each probe (COX I and β-actin genes) and 500 pg of DNA sample for mtDNA and 10 ng for nDNA. The PCR conditions were 95°C for 15 min, followed by 40 cycles of 95°C for 15 s, and 60°C for 60 s. The threshold cycle number (Ct) values of the β-actin gene and the mitochondrial COXI gene were determined.
The 4,977 bp deletion level was measured by real-time PCR on deletion product (Figure. 1) and normalized by simultaneous measurement of mtDNA COX I.
Each measurement was carried out in triplicate and normalized against a serial dilution of a control DNA sample and then the quantity of each target gene in our samples was calculated according to the corresponding standard curve.
The primer and probe information is as follows:
For mtDNA COX I: Forward, TTCGCCGACCGTTGACTATTCTCT; Reverse, AAGATTATTACAAATGCATGGGC.
For nuclear β-actin: Forward, ACCCACACTGTGCCCATCTAC; Reverse, TCGGTGAGGATCTTCATGAGGTA.
For mtDNA 4,977 bp deletion: Forward, CCTTACACTATTCCTCATCACC; Reverse, TGTGGTCTTTGGAGTAGAAACC
Probes: COX I, FAM-AACGACCACATCTACAACGTTATCGTCAC-ECLIPSE; β-actin, FAM-ATGCCCTCCCCCATGCCATCC-ECLIPSE. 4,977 -bp deletion, FAM-TGGCAGCCTAGCATTAGCAGG-ECLIPSE
Standard curves for deleted mtDNA were generated from a prostate cancer patient, and for COX I and β-actin genes they were obtained using a human osteosarcoma-derived cell line (U2OS) DNA. mtDNA content was calculated by dividing the amount of total mitochondrial DNA into the amount of nuclear gene. The mtDNA deletion level was expressed as the ratio of content of deleted mitochondrial DNA to total mtDNA content.
Categorical variables were analyzed using chi-square test or Fisher's exact test and continuous variables were examined using Student's t-test. In order to adjust for the contribution of each clinicopathologic characteristic, logistic regression and linear regression analysis were also performed. All statistical results were calculated with SPSS 16.0 software and a p value less than 0.05 was considered statistically significant.
Detection of the mtDNA 4,977 bp deletion in colorectal cancer patients
Detection of the mtDNA 4,977 bp deletion in tumor and non-tumor tissues of 104 colorectal cancer patients.
Deletion detected in non-tumor tissues
OR (95% CI)
Deletion detected in tumor tissues
OR (95% CI)
Level of the 4,977 bp deletion and colorectal cancer development
Multiple linear regression analysis of the relationship between mtDNA 4,977 bp deletion level and age, BMI, metastasis status and stage of cancer in tumor and non-tumor tissues in 20 patients with the deletion.
95% CI for B
-0.002 to 0.001
-0.003 to 0.004
-0.074 to 0.029
-0.043 to 0.020
0.000 to 0.000
0.000 to 0.001
-0.014 to 0.002
-0.010 to 0.000
The 4,977 bp deletion level and mtDNA copy number in colorectal cancer patients
mtDNA copy number as a potential marker in colorectal cancer
Multiple linear regression analysis of relationships between mtDNA content and age, metastasis status and stage of cancer in tumor and non-tumor tissues in patients with or without the 4,977 bp mtDNA deletion
Patients without 4977 bp deletion
Patients with 4977 bp deletion
95% CI for B
95% CI for B
-0.220 to 0.602
-0.055 to 0.108
-0.328 to 50.386
-1.464 to 5.739
-1.759 to 30.679
-0.614 to 3.993
-0.052 to 0.087
-0.033 to 0.161
-4.619 to 3.959
3.578 to 12.182
-2.844 to 2.643
3.088 to 8.590
mtDNA mutations have been implicated in various human diseases including cancer , a long-term process that involves multiple steps driven by different genetic and epigenetic alterations. Among the mtDNA mutations, the 4,977-bp deletion is one of the most frequent . Several studies have found the mtDNA 4,977-bp deletion in various types of cancer, including in cancer of the breast, endometrial, esophagus, stomach, head and neck, liver, lung, mouth, kidney, skin and thyroid [24, 28]. However, in some cases, the incidence and level of the 4,977 bp deletion were lower in the tumor tissues compared with nearby non-tumor tissues from the same patients. Thus, the role of this common deletion in tumorigenesis is intriguing, but largely perplexing.
In our first finding, unlike the age-dependent accumulation that was expected, the 4,977 bp deletion was detected more frequently in tumor tissues of patients younger than 65 (12/48, or 25%) compared to patients over 65 (5/56, or 8.9%) (p = 0.027). This result indicated that there is possibly a negative selection for the common deletion in tumor tissues during aging. In addition, as previously reported with thyroid, renal and liver cancer patients , we found the deletion level in tumor tissues was likely to be lower than that in the nearby non-tumor areas. In particular, in 10 patients carrying the 4,977-bp deletion in both tumor and nearby non-tumor tissues, the deletion levels were almost all lower in tumor tissues, indicating a negative selection for the common deletion in the cancer cells. It is also interesting to note that among these 10 subjects, patient 199 was at an advanced tumor stage and exhibited metastatic features, and showed the biggest difference in the deletion levels between tumor and non-tumor tissues and the highest level of the common deletion in the non-tumor areas (Figure. 2B). On the other hand, patient 137 exhibited very similar deletion level in tumor tissues and the nearby non-tumor tissues (Figure. 2B), and he was at the early stage of cancer and exhibited no metastasis. Furthermore, among all 17 patients who carried the deletion in tumor tissues, there was a good correlation after multiple linear regression analysis (p = 0.031) between the decrease in deletion level in tumor tissues and the stage of advancement in the cancer.
To explain the observed results, we hypothesize that, as we previously found in tumorigenesis studies on cells carrying mtDNA with heteroplasmic and homoplasmic mutations in the complex I subunit ND5 gene , the 4,977-bp mtDNA deletion could function in cancer development as follows: in the initial stage, when cancer cells are under stress because of a carcinogenic insult or oxidative stress damage, the deletion emerges. Because of the replicative advantage of smaller mtDNA, mtDNA with the 4,977-bp deletion is enriched to a certain level which would enhance tumor progression due to retrograde pathways . Retrograde regulation is a communication pathway from mitochondria to the nucleus, and is usually used to describe the cellular responses to changes in the functional state of mitochondria. One of the mechanisms suggested to play a role in the retrograde response was mitochondrial stress, which is supported by changes in mitochondrial membrane potential and calcium elevation . However, at certain stage of tumorigenesis, it may become more important to have a functional respiratory chain than an inhibited one to sustain rapid cell proliferation. As a result, compared with the nearby non-tumor tissues, mtDNA with the common deletion becomes diluted out in tumor tissues.
Low mtDNA content has been reported to be associated with increased risk of renal cancer carcinoma , and a decrease in mtDNA copy number in cancer tissues has been found also in gastric cancer , breast cancer  and hepatocellular carcinoma . On the other hand, an increase in mtDNA content was reported in the majority of renal oncocytomas , head and neck cancer , endometrial cancer , ovarian cancer  and colorectal cancer . It is therefore suggested that the change in mtDNA content is cancer type specific [28, 34]. However the underlying molecular mechanisms of alteration in mtDNA in cancer cells are largely unclear.
Our results strongly suggest that the 4,977-bp deletion is important in the specific up-regulation of mtDNA content in tumor tissues. The mtDNA content in tumor tissue of almost all of 20 patients who carried the 4,977-bp deletion was higher than that in the nearby non-tumor areas. The only exception was patient 137 (Figure. 4A), who was in her early stage of cancer and without detection of metastasis in the lymph nodes. On the other hand, patient 180 (Figure. 4A), who showed the biggest difference in the levels of mtDNA content between tumor and non-tumor areas, was in the terminal stage of cancer and with metastasized lymph nodes. These results support the notion that tumor background favors high mtDNA copy number with the presence of the 4,977-bp deletion. As this deletion removes all or part of the genes encoding four complex I subunits, one complex IV subunit, two complex V subunits and five tRNA genes, which are indispensable for maintaining normal mitochondrial function , the mtDNA 4,977-bp deletion could lead to energy production catastrophes  and abnormal ROS generation . Since the deletion levels observed here were well below the threshold for any bioenergetics consequences , the up-regulation is more likely due to a retrograde reaction  rather than a simple compensatory effect for ATP production. This retrograde signal is amplified in a colorectal cancer background.
In conclusion, our investigation provides evidence that the mtDNA 4,977-bp deletion plays a role in the early stage of colorectal cancer, but it is selected against once the tumor enter the rapid growth phase. Our results also demonstrate that in tumors with this common deletion, mtDNA content could increase specifically in tumor tissues, probably due to a retrograde effect. These results also indicate that both the 4,977-bp deletion and mtDNA content may serve as a biomarker for colorectal cancer in some patients.
This work is supported by Chinese National Science Foundation (31070765/C050605, and 810004611/H1409), the Major State Basic Research Development Program of China (No.2007CB507400), and Yidong Bai is supported by a NIH grant (R21NS072777).
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