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Association between insertion/deletion polymorphism in angiotensin-converting enzyme gene and acute lung injury/acute respiratory distress syndrome: a meta-analysis
© Matsuda et al.; licensee BioMed Central Ltd. 2012
Received: 20 November 2011
Accepted: 9 August 2012
Published: 31 August 2012
A previous meta-analysis reported a positive association between an insertion/deletion (I/D) polymorphism in the angiotensin-converting enzyme gene (ACE) and the risk of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Here, we updated this meta-analysis and additionally assessed the association of this polymorphism with ALI/ARDS mortality.
We searched electronic databases through October 2011 for the terms “angiotensin-converting enzyme gene”, “acute lung injury”, and “acute respiratory distress syndrome,” and reviewed all studies that reported the relationship of the I/D polymorphism in ACE with ALI/ARDS in humans. Seven studies met the inclusion criteria, comprising 532 ALI/ARDS patients, 3032 healthy controls, and 1432 patients without ALI/ARDS. We used three genetic models: the allele, dominant, and recessive models.
The ACE I/D polymorphism was not associated with susceptibility to ALI/ARDS for any genetic model. However, the ACE I/D polymorphism was associated with the mortality risk of ALI/ARDS in Asian subjects ( P allele < 0.0001, P dominant = 0.001, P recessive = 0.002). This finding remained significant after correction for multiple comparisons.
There is a possible association between the ACE I/D polymorphism genotype and the mortality risk of ALI/ARDS in Asians.
Acute lung injury (ALI) and its most severe form called acute respiratory distress syndrome (ARDS) are characterized by increased permeability of the alveolar-capillary barrier resulting in edema, excessive inflammatory responses, and interstitial fibrosis in the lung, thus impairing arterial oxygen exchange [1, 2]. Despite innovations in intensive care medicine, the mortality of ARDS remains up to 40% . Therefore, clarification of the unknown pathophysiology and the development of effective therapeutics are urgently needed to overcome the life-threatening conditions induced by ALI/ARDS. It has been suggested that the type and severity of injury may affect the occurrence of ALI/ARDS; however, it remains unclear why up to 50% of individuals are not affected despite experiencing similar injuries [3, 4]. Several genetic variants have been suggested to be associated with the development and progression of ALI/ARDS (reviewed by Flores et al. ).
The human angiotensin-converting enzyme (ACE) gene (ACE) is located on chromosome 17q23 and contains an insertion/deletion (I/D) polymorphism of a 287-bpAlu repeat sequence in intron 16 [6, 7]. Recently, strong evidence has accumulated for a pathophysiological association between lung and kidney diseases and the ACE I/D polymorphism, which is correlated with circulating and cellular ACE levels [8–12]. This evidence has been rendered using a meta-analysis approach that provided the most reliable compilation of the data currently available in the field [13, 14]. The studies of Marshall et al. , Jerng et al. , Lu et al. , and Adamzik et al.  have shown a significant positive association between the ACE I/D polymorphism and the risk and/or mortality of ALI/ARDS, although the studies of Chan et al. , Plunkett et al. , and Villar et al.  failed to detect a positive association (Additional file 1: Table S1). This association therefore remains controversial. The discrepancy among these results may be because of different sample sizes, because the sample sizes of these studies were quite small for genetic association analysis. To overcome this limitation, Hu et al.  conducted a meta-analysis and reported that the ACE I/D polymorphism was associated with the risk of ALI/ARDS. However, we have noticed that two relevant studies were not included in this meta-analysis. Hence, to obtain a reliable conclusion regarding the association between the ACE I/D polymorphism and ALI/ARDS risk, we conducted an updated systematic review and meta-analysis of all relevant publications, increasing the sample size from 3133 (in Hu et al.) to 4996. In addition, we carried out a meta-analysis between the ACE I/D polymorphism and the mortality of ALI/ARDS.
We performed this meta-analysis according to the guidelines of Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA; 2009) .
Data sources and searches
To identify eligible studies, we (A.M. and T.K.) independently searched PubMed, Embase, the Cochrane Library, and Google Scholar citations through October 2011 using as key words the terms “angiotensin-converting enzyme gene” or “ACE gene”; “acute lung injury” or “ALI”; and “acute respiratory distress syndrome” or “ARDS”.
Study selection and eligibility criteria
We defined inclusion and exclusion criteria a priori. The inclusion criteria were: the study should (1) be published in a peer-reviewed journal, (2) contain independent data of ACE I/D genotype, (3) have the outcome as either development of ALI/ARDS or ALI/ARDS mortality, and (4) be a case–control study in human subjects. ALI/ARDS was defined as a lung disease with acute onset, non-cardiac diffuse bilateral pulmonary infiltrates, and a PaO2/FiO2 ≤ 300 for ALI and a PaO2/FiO2 ≤ 200 for ARDS . Studies were excluded if they were duplicates. Studies included in the meta-analysis were not restricted by language.
The reference lists of reviews and retrieved articles were verified independently by two investigators (A.M. and T.K.). The following information was extracted from each article: first author’s name, year of publication, ethnicity, patient group, age, sex, number of cases and controls, type and number of lung injury patients (ALI or ARDS) and controls for each ACE I/D genotype, and mortality at each assessment time. The frequencies of alleles were calculated for cases and controls from the corresponding genotype distributions. Disagreement was resolved by discussion.
We conducted a meta-analysis between the ACE I/D polymorphism and the risk and mortality of ALI/ARDS. Because the mode of inheritance of ALI/ARDS is unknown, we performed a meta-analysis with the allele (I allele versus D allele), dominant (I/I versus I/D + D/D), and recessive (I/I + I/D versus D/D) models (as recommended in ). The meta-analysis was performed using Review Manager Version 5.0 for Windows (Cochrane Collaboration, http://www.cc-ims.net/RevMan). Cochran’s chi-square-based Q-statistic test was applied to assess between-study heterogeneity. We included healthy subjects and patients without ALI/ARDS (e.g., intensive care unit patients who did not fulfill the criteria for ALI/ARDS) as separate controls. The pooled odds ratios (OR) were calculated using DerSimonian-Laird random-effects models  with 95% confidence intervals (CI) to measure the strength of the association. I2 indicates the percentage variance in the pooled OR that can be attributed to heterogeneity. Values of 25% are considered low, 50% moderate, and 75% high. We applied the conservative random effects model for all comparisons because the underlying effect may differ across studies and among heterogeneous populations. When we searched the HapMap database, we identified different linkage disequilibrium groups around the ACE gene among different ethnicities. Therefore, we conducted additional sensitivity analyses, separating the Caucasian and Asian participants. Finally, Bonferroni’s correction was used to control the type I error rate in the meta-analysis of mortality. We employed 18 separate tests (three genotype models: allele, dominant, and recessive; three populations: total, Caucasians, and Asians; and two traits: susceptibility and mortality); therefore, we calculated the corrected P value by multiplying the original P value by 18. P was considered significant at less than 0.05.
Publication bias was assessed using WINPEPI software (http://www.brixtonhealth.com/pepi4windows.html), by visually examining a funnel plot and formally assessing asymmetry with both the Egger test  and the rank correlation test .
Information on the studies included in the meta-analysis
Age, yrs ± SD or (range)
Male sex, n (%)
Healthy british men
“ICU control”; admitted to ICU for non-ARDS respiratory failure with mechanical ventilation (n=88). “CABG control”; admitted to ICU following CABG, but without respiratory failure (n=174).
Healthy individuals under routine health check
SARS patients without ARDS
“At-risk”; admitted to ICU with acute respiratory failure but not ARDS (n=138). “Non-at-risk”; no history of respiratory failure or hospitalization within 6M (n=210).
Healthy Caucasian individuals of either sex
Severe septic patients without ARDS but had some degree of repiartory failure.
Admitted to ICU for ventilatory and/or inotropic support without ARDS (n=199)
Healthy blood donors
Admitted to the hospital over the same period due to other diseases (n=408).
Number of pooled studies in each phenotype
ACE I/D polymorphism and susceptibility to ALI/ARDS
ALI/ARDS patients versus healthy control subjects
Initially, we conducted a meta-analysis of ALI/ARDS risk using healthy control subjects, because this approach is more powerful than using other controls to confirm the association of genotype with disease susceptibility. Two studies [16, 20] did not include healthy subjects as control in their analysis; therefore, we used the remaining five [15, 17–19, 21], comprising 418 ALI/ARDS patients and 3032 healthy control subjects. We found significant heterogeneities among the ORs (P allele < 0.0001, P dominant = 0.003, and P recessive = 0.004) (Additional file 2: Figure S1, Additional file 3: Figure S2, Additional file 4: Figure S3). The pooled OR derived from the five studies [15, 17, 19, 21, 29] did not indicate significant association for any genotype model (Additional file 2: Figure S1, Additional file 3: Figure S2, Additional file 4: Figure S3). There was no significant publication bias using either the Egger test or the rank correlation test (data not shown). To limit the ethnic heterogeneity, we separated the studies into those with either Caucasian or Asian samples. In the three studies with Caucasian subjects [15, 18, 21], comprising 300 patients and 2470 healthy controls, we found significant heterogeneity among the ORs (P allele < 0.0001, P dominant = 0.001, P recessive = 0.0006) (Additional file 2: Figure S1, Additional file 3: Figure S2, Additional file 4: Figure S3). The pooled OR indicated no significant association (Additional file 2: Figure S1, Additional file 3: Figure S2, Additional file 4: Figure S3). In the two studies with Asian subjects [17, 19], comprising 118 patients and 562 healthy controls, there was no significant heterogeneity among the ORs (Additional file 2: Figure S1, Additional file 3: Figure S2, Additional file 4: Figure S3). The I/D polymorphism in ACE was not associated with susceptibility to ALI/ARDS in Asian subjects (Additional file 2: Figure S1, Additional file 3: Figure S2, Additional file 4: Figure S3).
ALI/ARDS patients versus patients without ALI/ARDS
One study  did not include patients without ALI/ARDS as a control in the analysis; therefore, we used the remaining six [15–17, 19–21], comprising 448 ALI/ARDS patients and 1432 patients without ALI/ARDS. There were significant heterogeneities among the ORs (P allele = 0.0006, P dominant = 0.004, and P recessive = 0.03) (Additional file 5: Figure S4, Additional file 6: Figure S5, Additional file 7: Figure S6). The pooled OR derived from the six studies [15–17, 19–21] did not indicate significant association for any genotype model (Additional file 5: Figure S4, Additional file 6: Figure S5, Additional file 7: Figure S6). There was no significant publication bias using either the Egger test or the rank correlation test (data not shown). We then conducted an additional analysis to limit the ethnic heterogeneity. In the three studies with Caucasian subjects [15, 20, 21], comprising 229 ALI/ARDS patients and 553 patients without ALI/ARDS, we found significant heterogeneity among the ORs (P allele = 0.0009, P dominant = 0.03, and P recessive = 0.002) (Additional file 5: Figure S4, Additional file 6: Figure S5, Additional file 7: Figure S6). The pooled OR derived from the three studies [15, 20, 21] indicated no significant association for any genotype model (Additional file 5: Figure S4, Additional file 6: Figure S5, Additional file 7: Figure S6). In the three studies with Asian subjects [16, 17, 19], comprising 219 ALI/ARDS patients and 879 patients without ALI/ARDS, the I/D polymorphism in ACE was not associated with ALI/ARDS risk. There was no evidence of between-study heterogeneity (Additional file 5: Figure S4, Additional file 6: Figure S5, Additional file 7: Figure S6).
ACE I/D polymorphism and mortality of ALI/ARDS
The renin-angiotensin system (RAS) has been well documented to contribute to the pathophysiology of ALI/ARDS by increasing vascular permeability . ACE is a key enzyme of the RAS that converts inactive angiotensin I to the vasoactive and aldosterone-stimulating peptide angiotensin II that metabolizes kinins and many other biologically active peptides . ACE is found in varying amounts on the surface of lung epithelial and endothelial cells. Activation of the RAS can stimulate production of tumor necrosis factor alpha in cardiac fibroblasts . Angiotensin II induces apoptosis of lung epithelial and endothelial cells and is a potent fibrogenic factor [33–36]. Based on these biological properties of ACE, there is considerable interest in its potential involvement in ALI/ARDS.
Despite conflicting findings among the genetic association studies, a recent meta-analysis  suggested a positive association between the ACE I/D polymorphism and ARDS susceptibility among Caucasians. Here, we have extended this meta-analysis to a much larger sample size (532 ALI/ARDS patients, 3032 healthy controls, and 1432 patients without ALI/ARDS), and failed to detect an association between the ACE I/D polymorphism and ALI/ARDS risk. The discrepancy may be because of the different methodology used and the different sample size. We believe that our result is more convincing because we added two studies to those used by Hu et al.  and also used three genetic models, as recommended in the literature . Furthermore, we extended our meta-analysis to examine mortality, and detected a significant association between the ACE I/D polymorphism and the mortality risk of ALI/ARDS in Asian populations.
ALI/ARDS patients had a higher frequency of the D/D genotype than controls did (Additional file 1: Table S1). The ACE I/D polymorphism has been associated with 28–47% of the variance in circulating ACE levels in healthy subjects, and high plasma ACE levels are associated with the D/D genotype [6, 7]. It is therefore possible that the high plasma ACE levels produced by having the D/D genotype affect the mortality of ALI/ARDS. Recently, ACE inhibitors have been reported to attenuate ALI induced by bleomycin, acid, and endotoxin in animal models [37–39]. Taken together, patients at risk of mortality from ALI/ARDS, as determined by ACE I/D polymorphism screening, may be good candidates for treatment with ACE inhibitors. Furthermore, because our results suggest that the ACE I/D polymorphism genotype might influence treatment outcome through differences in drug metabolism and activity, it will be important to investigate the pharmacogenomics of ALI/ARDS and gene–gene interactions in the RAS to clarify the role of different RAS genes in the pathophysiology and treatment response of ALI/ARDS.
We did not find an association between the ACE I/D polymorphism and ALI/ARDS mortality in the total population. However, there were significant heterogeneities among the ORs. This heterogeneity may be derived from: (1) different ancestries, (2) incomplete genotyping or genotyping error differences among the studies, and (3) a relatively small total sample size (196 survivors and 210 non-survivors). Differences in the genotype distributions have been reported between Caucasians and Asians, which may support the above explanations (the studies of Villar et al. and Adamzik et al. reported that the major allele was “Deletion”, but other Asian studies showed that the major allele was “Insertion”). Therefore, we stratified the population into Caucasian and Asian subjects, thereby eliminating the significant heterogeneity among the ORs for both ethnicities. After stratification, we detected a significant association between the ACE I/D polymorphism and ALI/ARDS mortality in Asian populations. D/D was the risk genotype for mortality in Asian ALI/ARDS patients.
Several limitations of our meta-analysis remain, e.g., a small sample size and differences in the quality control among the studies. Neither the mode of inheritance nor the heritability of ALI/ARDS is known. Therefore, we used three genetic models to obtain more statistical power than in previous individual studies. Based on the common disease-common variant hypothesis, and assuming ALI/ARDS to be a complex disease, at least 4000 samples ( i.e., 2000 cases and 2000 controls) are required to obtain sufficient statistical power (GRR = 2.0 and P = 5 × 10-7) . Given that our study is under-powered because of its small sample size, we may have failed to detect a genuine association between the ACE I/D polymorphism genotype and ALI/ARDS susceptibility. A replication study using a larger sample size, and/or samples from other populations, is required to obtain conclusive results.
In conclusion, our results indicate that the genotype of the I/D polymorphism in ACE may be a predictor of ALI/ARDS mortality in Asian populations. However, more case-control association investigations on larger, stratified populations are required to clarify the role of this polymorphism in ALI/ARDS risk and mortality.
We thank Dr. Rachel Agbeko for providing the genotype information. This work was supported in part by research grants from the Japanese Ministry of Education, Culture, Sports, Science and Technology, the Ministry of Health, Labour and Welfare, and the Health Sciences Foundation (Research on Health Sciences focusing on Drug Innovation). Dr. Kishi is a postdoctoral fellow for research abroad, and is additionally supported by the Japan Research Foundation for Clinical Pharmacology and the Ministry of Education.
- Ware LB: Pathophysiology of acute lung injury and the acute respiratory distress syndrome. Semin Respir Crit Care Med. 2006, 27: 337-349. 10.1055/s-2006-948288.View ArticlePubMedGoogle Scholar
- Ware LB, Matthay MA: The acute respiratory distress syndrome. N Engl J Med. 2000, 342: 1334-1349. 10.1056/NEJM200005043421806.View ArticlePubMedGoogle Scholar
- Bernard GR, Artigas A, Brigham KL, Carlet J, Falke K, Hudson L, Lamy M, Legall JR, Morris A, Spragg R: The American-European Consensus Conference on ARDS. Definitions, mechanisms, relevant outcomes, and clinical trial coordination. Am J Respir Crit Care Med. 1994, 149: 818-824.View ArticlePubMedGoogle Scholar
- Hudson LD, Milberg JA, Anardi D, Maunder RJ: Clinical risks for development of the acute respiratory distress syndrome. Am J Respir Crit Care Med. 1995, 151: 293-301.View ArticlePubMedGoogle Scholar
- Flores C, Pino-Yanes MM, Casula M, Villar J: Genetics of acute lung injury: past, present and future. Minerva Anestesiol. 2010, 76: 860-864.PubMedGoogle Scholar
- Rigat B, Hubert C, Alhenc-Gelas F, Cambien F, Corvol P, Soubrier F: An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels. J Clin Invest. 1990, 86: 1343-1346. 10.1172/JCI114844.View ArticlePubMedPubMed CentralGoogle Scholar
- Tiret L, Rigat B, Visvikis S, Breda C, Corvol P, Cambien F, Soubrier F: Evidence, from combined segregation and linkage analysis, that a variant of the angiotensin I-converting enzyme (ACE) gene controls plasma ACE levels. Am J Hum Genet. 1992, 51: 197-205.PubMedPubMed CentralGoogle Scholar
- Hu Z, Jin X, Kang Y, Liu C, Zhou Y, Wu X, Liu J, Zhong M, Luo C, Deng L, et al: Angiotensin-converting enzyme insertion/deletion polymorphism associated with acute respiratory distress syndrome among caucasians. J Int Med Res. 2010, 38: 415-422.View ArticlePubMedGoogle Scholar
- Qi Y, Sun J, Zhu T, Wang W, Liu J, Zhou W, Qiu C, Zhao D: Association of angiotensin-converting enzyme gene insertion/deletion polymorphism with high-altitude pulmonary oedema: a meta-analysis. J Renin Angiotensin Aldosterone Syst. 2011, 12: 617-623. 10.1177/1470320311406572.View ArticlePubMedGoogle Scholar
- Qin YH, Zhou TB, Su LN, Lei FY, Huang WF, Zhao YJ: Association between ACE polymorphism and risk of IgA nephropathy: a meta-analysis. J Renin Angiotensin Aldosterone Syst. 2011, 12: 215-223. 10.1177/1470320310391835.View ArticlePubMedGoogle Scholar
- Zhou TB, Qin YH, Su LN, Lei FY, Huang WF, Zhao YJ: Relationship between Angiotensin-Converting Enzyme Insertion/Deletion Gene Polymorphism and Susceptibility of Minimal Change Nephrotic Syndrome: A Meta-Analysis. Int J Nephrol. 2011, 360357-http://www.ncbi.nlm.nih.gov/pubmed?term=Relationship%20between%20%20Angiotensin-Converting%20Enzyme%20Insertion%2FDeletion%20Gene%20Polymorphism%20and%20%20Susceptibility%20of%20Minimal%20Change%20Nephrotic%20Syndrome%3A%20A%20Meta-Analysis,Google Scholar
- Zhou TB, Qin YH, Su LN, Lei FY, Huang WF, Zhao YJ, Pang YS, Yang KP: The association between angiotensin-converting enzyme insertion/deletion gene variant and risk of focal segmental glomerulosclerosis: a systematic review and meta-analysis. J Renin Angiotensin Aldosterone Syst. 2011, 12: 624-633. 10.1177/1470320311410584.View ArticlePubMedGoogle Scholar
- Ioannidis JP, Ntzani EE, Trikalinos TA, Contopoulos-Ioannidis DG: Replication validity of genetic association studies. Nat Genet. 2001, 29: 306-309. 10.1038/ng749.View ArticlePubMedGoogle Scholar
- Kishi T, Tsunoka T, Ikeda M, Kawashima K, Okochi T, Kitajima T, Kinoshita Y, Okumura T, Yamanouchi Y, Inada T, et al: Serotonin 1A receptor gene and major depressive disorder: an association study and meta-analysis. J Hum Genet. 2009, 54: 629-633. 10.1038/jhg.2009.84.View ArticlePubMedGoogle Scholar
- Marshall RP, Webb S, Bellingan GJ, Montgomery HE, Chaudhari B, McAnulty RJ, Humphries SE, Hill MR, Laurent GJ: Angiotensin converting enzyme insertion/deletion polymorphism is associated with susceptibility and outcome in acute respiratory distress syndrome. Am J Respir Crit Care Med. 2002, 166: 646-650. 10.1164/rccm.2108086.View ArticlePubMedGoogle Scholar
- Jerng JS, Yu CJ, Wang HC, Chen KY, Cheng SL, Yang PC: Polymorphism of the angiotensin-converting enzyme gene affects the outcome of acute respiratory distress syndrome. Crit Care Med. 2006, 34: 1001-1006. 10.1097/01.CCM.0000206107.92476.39.View ArticlePubMedGoogle Scholar
- Lu XM, Chen GJ, Yang Y, Qiu HB: Angiotensin-converting enzyme polymorphism affects outcome of local Chinese with acute lung injury. Respir Med. 2011, 105: 1485-1490. 10.1016/j.rmed.2011.06.009.View ArticlePubMedGoogle Scholar
- Adamzik M, Frey U, Sixt S, Knemeyer L, Beiderlinden M, Peters J, Siffert W: ACE I/D but not AGT (−6)A/G polymorphism is a risk factor for mortality in ARDS. Eur Respir J. 2007, 29: 482-488. 10.1183/09031936.00046106.View ArticlePubMedGoogle Scholar
- Chan KC, Tang NL, Hui DS, Chung GT, Wu AK, Chim SS, Chiu RW, Lee N, Choi KW, Sung YM, et al: Absence of association between angiotensin converting enzyme polymorphism and development of adult respiratory distress syndrome in patients with severe acute respiratory syndrome: a case control study. BMC Infect Dis. 2005, 5: 26-10.1186/1471-2334-5-26.View ArticlePubMedPubMed CentralGoogle Scholar
- Plunkett A, Agbeko RS, Li K, Humphries SE, Klein NJ, Peters MJ: Angiotensin-converting enzyme D allele does not influence susceptibility to acute hypoxic respiratory failure in children. Intensive Care Med. 2008, 34: 2279-2283. 10.1007/s00134-008-1260-z.View ArticlePubMedGoogle Scholar
- Villar J, Flores C, Perez-Mendez L, Maca-Meyer N, Espinosa E, Blanco J, Sanguesa R, Muriel A, Tejera P, Muros M, et al: Angiotensin-converting enzyme insertion/deletion polymorphism is not associated with susceptibility and outcome in sepsis and acute respiratory distress syndrome. Intensive Care Med. 2008, 34: 488-495. 10.1007/s00134-007-0937-z.View ArticlePubMedGoogle Scholar
- Moher D, Liberati A, Tetzlaff J, Altman DG: Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. BMJ. 2009, 339: b2535-10.1136/bmj.b2535.View ArticlePubMedPubMed CentralGoogle Scholar
- Wheeler AP, Bernard GR: Acute lung injury and the acute respiratory distress syndrome: a clinical review. Lancet. 2007, 369: 1553-1564. 10.1016/S0140-6736(07)60604-7.View ArticlePubMedGoogle Scholar
- Zintzaras E: The generalized odds ratio as a measure of genetic risk effect in the analysis and meta-analysis of association studies. Stat Appl Genet Mol Biol. 2010, 9: Article21-PubMedGoogle Scholar
- DerSimonian R, Laird N: Meta-analysis in clinical trials. Control Clin Trials. 1986, 7: 177-188. 10.1016/0197-2456(86)90046-2.View ArticlePubMedGoogle Scholar
- Abramson JH: WINPEPI updated: computer programs for epidemiologists, and their teaching potential. Epidemiol Perspect Innov. 2011, 8: 1-10.1186/1742-5573-8-1.View ArticlePubMedPubMed CentralGoogle Scholar
- Egger M: Davey Smith G, Schneider M, Minder C: Bias in meta-analysis detected by a simple, graphical test. BMJ. 1997, 315: 629-634. 10.1136/bmj.315.7109.629.View ArticlePubMedPubMed CentralGoogle Scholar
- Begg CB, Mazumdar M: Operating characteristics of a rank correlation test for publication bias. Biometrics. 1994, 50: 1088-1101. 10.2307/2533446.View ArticlePubMedGoogle Scholar
- Adamzik M, Frey UH, Rieman K, Sixt S, Beiderlinden M, Siffert W, Peters J: Insertion/deletion polymorphism in the promoter of NFKB1 influences severity but not mortality of acute respiratory distress syndrome. Intensive Care Med. 2007, 33: 1199-1203. 10.1007/s00134-007-0649-4.View ArticlePubMedGoogle Scholar
- Yamamoto T, Wang L, Shimakura K, Sanaka M, Koike Y, Mineshita S: Angiotensin II-induced pulmonary edema in a rabbit model. Jpn J Pharmacol. 1997, 73: 33-40. 10.1254/jjp.73.33.View ArticlePubMedGoogle Scholar
- Lambert DW, Clarke NE, Turner AJ: Not just angiotensinases: new roles for the angiotensin-converting enzymes. Cell Mol Life Sci. 2010, 67: 89-98. 10.1007/s00018-009-0152-x.View ArticlePubMedGoogle Scholar
- Yokoyama T, Sekiguchi K, Tanaka T, Tomaru K, Arai M, Suzuki T, Nagai R: Angiotensin II and mechanical stretch induce production of tumor necrosis factor in cardiac fibroblasts. Am J Physiol. 1999, 276: H1968-1976.PubMedGoogle Scholar
- Behnia R, Molteni A, Igic R: Angiotensin-converting enzyme inhibitors: mechanisms of action and implications in anesthesia practice. Curr Pharm Des. 2003, 9: 763-776. 10.2174/1381612033455413.View ArticlePubMedGoogle Scholar
- Igic R, Behnia R: Properties and distribution of angiotensin I converting enzyme. Curr Pharm Des. 2003, 9: 697-706. 10.2174/1381612033455459.View ArticlePubMedGoogle Scholar
- Imai Y, Kuba K, Rao S, Huan Y, Guo F, Guan B, Yang P, Sarao R, Wada T, Leong-Poi H, et al: Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature. 2005, 436: 112-116. 10.1038/nature03712.View ArticlePubMedGoogle Scholar
- Wang R, Zagariya A, Ibarra-Sunga O, Gidea C, Ang E, Deshmukh S, Chaudhary G, Baraboutis J, Filippatos G, Uhal BD: Angiotensin II induces apoptosis in human and rat alveolar epithelial cells. Am J Physiol. 1999, 276: L885-889.PubMedGoogle Scholar
- Hagiwara S, Iwasaka H, Matumoto S, Hidaka S, Noguchi T: Effects of an angiotensin-converting enzyme inhibitor on the inflammatory response in in vivo and in vitro models. Crit Care Med. 2009, 37: 626-633. 10.1097/CCM.0b013e3181958d91.View ArticlePubMedGoogle Scholar
- He X, Han B, Mura M, Xia S, Wang S, Ma T, Liu M, Liu Z: Angiotensin-converting enzyme inhibitor captopril prevents oleic acid-induced severe acute lung injury in rats. Shock. 2007, 28: 106-111. 10.1097/SHK.0b013e3180310f3a.View ArticlePubMedGoogle Scholar
- Wang R, Ibarra-Sunga O, Verlinski L, Pick R, Uhal BD: Abrogation of bleomycin-induced epithelial apoptosis and lung fibrosis by captopril or by a caspase inhibitor. Am J Physiol Lung Cell Mol Physiol. 2000, 279: L143-151.PubMedGoogle Scholar
- Sawcer S: Bayes factors in complex genetics. Eur J Hum Genet. 2010, 18: 746-750. 10.1038/ejhg.2010.17.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2350/13/76/prepub
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