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Low incidence of limb-girdle muscular dystrophy type 2C revealed by a mutation study in Japanese patients clinically diagnosed with DMD
© Okizuka et al; licensee BioMed Central Ltd. 2010
Received: 27 July 2009
Accepted: 30 March 2010
Published: 30 March 2010
Limb-girdle muscular dystrophy type 2C (LGMD2C) is an autosomal recessive muscle dystrophy that resembles Duchenne muscular dystrophy (DMD). Although DMD is known to affect one in every 3500 males regardless of race, a widespread founder mutation causing LGMD2C has been described in North Africa. However, the incidence of LGMD2C in Japanese has been unknown because the genetic background remains uncharacterized in many patients clinically diagnosed with DMD.
We enrolled 324 patients referred to the Kobe University Hospital with suspected DMD. Mutations in the dystrophin or the SGCG genes were analyzed using not only genomic DNA but also cDNA.
In 322 of the 324 patients, responsible mutations in the dystrophin were successfully revealed, confirming DMD diagnosis. The remaining two patients had normal dystrophin expression but absence of γ-sarcoglycan in skeletal muscle. Mutation analysis of the SGCG gene revealed homozygous deletion of exon 6 in one patient, while the other had a novel single nucleotide insertion in exon 7 in one allele and deletion of exon 6 in the other allele. These mutations created a stop codon that led to a γ-sarcoglycan deficiency, and we therefore diagnosed these two patients as having LGMD2C. Thus, the relative incidence of LGMD2C among Japanese DMD-like patients can be calculated as 1 in 161 patients suspected to have DMD (2 of 324 patients = 0.6%). Taking into consideration the DMD incidence for the overall population (1/3,500 males), the incidence of LGMD2C can be estimated as 1 per 560,000 or 1.8 per million.
To the best of our knowledge, this is the first study to demonstrate a low incidence of LGMD2C in the Japanese population.
Duchenne muscular dystrophy (DMD; OMIM#310200) is the most common inherited muscular dystrophy, and affects 1 in every 3,500 males, regardless of race. DMD is caused by a mutation in the dystrophin gene on the short arm of the X chromosome and is characterized by the absence of dystrophin in skeletal muscle. Those affected by DMD develop muscle weakness by the age of 4 or 5, followed by progressive muscle wasting that ultimately leads to patients being wheelchair bound by the age of 12. In addition, calf hypertrophy and lumbar lordosis are also observed. DMD patients succumb to either cardiac or respiratory failure secondary to the disease during their twenties .
Limb-girdle muscular dystrophy type 2C (LGMD2C) (OMIM # 253700) is an autosomal recessive disorder caused by mutations in the SGCG gene, which encodes γ-sarcoglycan. It is characterized by a childhood onset of progressive muscular dystrophy. The mean age of onset is 5.3 years, and half of these patients lose ambulation by the age of 12. Calf hypertrophy and lumbar lordosis are common . Based on these clinical findings, LGMD2C is referred to as a severe childhood autosomal recessive muscular dystrophy or as a Duchenne muscular dystrophy (DMD)-like autosomal recessive disease .
Unlike DMD, LGMD2C shows geographical difference in its incidence. The highest incidence of LGMD2C has been reported in North Africa as a result of a founder mutation in the SGCG gene . Numerous studies have summarized the clinical and pathological features of LGMDs outside of North Africa. These studies have reported at least 19 subtypes, with 7 exhibiting autosomal dominant (LGMD1A to E) and 12 exhibiting autosomal recessive (LGMD2A to J) patterns of inheritance . Previous studies have determined the prevalence of LGMD to range from 8.1 per million in a nationwide study in The Netherlands  to 40 per million in a worldwide survey . However, the incidence of subtype LGMD2C has yet to be determined. In the Bulgarian Roma (Gypsy) population, one founder mutation has been reported to be common . Other than in the geographical areas associated with founder mutations, only limited numbers of LGMD2C cases have been reported. For example, only nine and seven LGMD2C patients have been described among large numbers of patients examined in Italy  and the USA , respectively.
Since differentiation of LGMD2C from DMD has not been considered a major problem in current clinical practice, strong efforts to differentiate the two conditions have not been made. Dystrophin restoration therapy for DMD by either inducing exon skipping [10, 11] or by suppressing nonsense mutations  appears to be close to clinical implementation. However, before there can be any clinical application of these technologies, it is essential that DMD be confirmed at the molecular level.
Kobe University Hospital contains a DMD clinic that examines patients suspected to have the disease from all over Japan, especially from the western part of the country. We herein report on two LDMD2C patients that were found among a group of 324 Japanese patients suspected to have DMD. We accordingly estimate the incidence of LGMD2C in the Japanese population to be 1 per 560,000.
Boys were enrolled in this cohort if they had been referred to the Kobe University Hospital with a tentative clinical DMD diagnosis based on strongly elevated levels of serum creatine kinase (CK) activity. Patients ranged in age from 0 - 7 years. We performed an extensive analysis on mutations in the dystrophin gene and were able to molecularly identify a mutation in the dystrophin gene in 322 patients with DMD-like disease (manuscript in preparation). However, in two other patients, no mutations were noted in the dystrophin gene. After obtaining informed consent from their parents, further examinations were conducted on these patients.
Muscle samples were obtained from the quadriceps of each patient. Standard histochemical stains including hematoxylin and eosin (H-E), Gomori trichrome, NADH tetrazolium reductase, succinate dehydrogenase, periodic acid-Schiff, acid phosphatase, adenosine triphosphatase at pH 4.3 and 9.4, cytochrome c oxidase, and alkaline phosphatase were conducted. Immunohistochemical stains for α- and β-dystroglycan; α-, β-, γ-, and δ-sarcoglycan; dystrophin; and merosin were performed using their respective monoclonal antibodies (Novocastra, Newcastle upon Tyne, United Kingdom, and Millipore, Billerica, MA, USA).
Genomic dosage of the exons of the SGCG gene was assessed by a semiquantitative multiplex PCR, as previously described . Eight fragments encompassing exons 1 to 8 of the SGCG gene and one fragment encompassing exon 2 of the α-dystroglycan gene were co-amplified using two PCR reactions that employed six sets of primers (Table 1). PCR products were separated by capillary electrophoresis (Agilent 2001 Bioanalyzer with DNA 1000 Lab Chips, Agilent Technologies, Palo Alto, CA, USA). The amount of PCR product derived from the SGCG exons was quantified by measuring their peak areas followed by calculating the ratio of these areas to that found for the α-dystroglycan exon 2.
The two male patients with clinical diagnosis of DMD were incidentally found to have marked elevations of serum CK levels (more than 50 times higher than control) in early childhood, despite a negative family history for muscular dystrophy. To confirm the clinical diagnosis of DMD, dystrophin gene mutations were extensively searched for using not only genomic DNA but also mRNA. However, no mutations could be identified, even when we included a deep intron mutation .
To date, our analysis of patients suspected to have DMD has revealed that two of the entire cohort examined can be regarded as having LGMD2C. Thus, the relative incidence of LGMD2C among Japanese patients suspected to have DMD can be calculated as 1 in 161 (2 of 324 patients = 0.6%). When the DMD incidence is taken into consideration for the overall population (1/3,500 males), the incidence of LGMD2C can be estimated as 1 per 560,000 or 1.8 per million.
This is the first comprehensive study that has been able to definitively clarify the incidence of LGMD2C among patients suspected to have DMD. In our cohort of 324 Japanese children clinically diagnosed with DMD, two were diagnosed as having LGMD2C. The incidence of LGMD2C among this cohort was calculated as 1 in 161 (0.6%) and the incidence in the Japanese population was estimated at 1 per 0.56 million people. The relative proportions of all LGMDs, including LGMD2C, have been previously reported for various regions of the world [5, 9]. However, these reports did not describe the relative prevalence of LGMD2C to DMD. The present study is the first to describe the incidence of LGMG2C in one race. The incidence of severe LGMDs was estimated to be 11.8% in a German study of patients with severe muscular dystrophy with early childhood onset  and about 8 to 12% in a Brazilian study of males with a clinical diagnosis of DMD . In comparison, our results indicate a much lower incidence (0.6%) of LGMD2C among patients suspected to have DMD. However, the low prevalence of LGMD2C in Japanese is in accordance with the few reports that have examined LCMD2C patients from Japan [18, 19]. Regardless of these differences, the potential presence of LGMD2C needs to be considered when making a differential diagnosis of DMD, even in Japan.
Although originally reported to be a severe autosomal recessive muscular dystrophy that resembles DMD, LGMD2C has since been reported to have a heterogenous clinical course, even with identical mutations . Initially, both of our LGMD2C patients were tentatively diagnosed as having DMD, even though both patients showed only very mild muscle weakness during the original observation period. A clinical hallmark for differentiating DMD and LGMD2C involves the inheritance pattern. However, this is impossible to determine in sporadic cases in males. In the current cases, the failure to determine any dystrophin gene mutations that were responsible for DMD led to immunohistochemical examination of muscle tissue. Quite unexpectedly, we found normal staining for dystrophin in these patients (Figure 1). This finding proved to be the key for diagnosing the complete or near-complete absence of γ-sarcoglycan deficiency, which led to our using genetic analysis to definitively prove the γ-sarcoglycan deficiency.
It has been reported that residual sarcoglycan expression is highly variable, and that this makes it difficult to accurately predict the genotype [2, 20]. In addition, both sarcoglycanopathy and DMD were reported to show weak staining of all types of sarcoglycan complexes . On the other hand, patients with LGMD2C have been reported to show a significant reduction or a complete absence of γ-sarcoglycan staining in conjunction with reduced or only partially preserved staining of the other sarcoglycan proteins [2, 5, 9, 21, 22]. In the present two cases, we observed a marked reduction of γ-sarcoglycan, in addition to finding a reduction of staining intensity for other members of the sarcoglycan complex (Figure 1).
Since the maintenance of the carboxyl terminus of γ-sarcoglycan is important for both the processing and stability of the protein, mutations in the extracellular domain of γ-sarcoglycan can lead to an absence of protein expression . While the most common cause of LGMD2C is thought to be a homozygous del521T in exon 6 of the SGCG gene, this mutation has not been reported in Japanese. However, in the current study we identified a novel c.602_603insT mutation. We identified a homozygous deletion of exon 6 of the SGCG gene in patient 1. We also identified the exon 6 deletion in three of four alleles in our two Japanese LGMD2C patients. This mutation has been previously reported to occur as a hemizygous condition in Japanese . In Europe, this exon 6 deletion has been reported in both homozygous and hemizygous conditions . Taken together, these previous findings suggest that exon 6 can be considered prone to deletions. Although our identification of the exon 6 deletion in one allele was initially difficult, we have now been able to successfully detect deletions in one allele by using semiquantitative PCR amplification (Figure 3). Therefore, use of this methodology may help to increase the mutation detection rate in patients suspected to have DMD, and in addition, help to correctly identify LGMD2C. Being able to successfully identify LGMD2C patients in the future will help to ensure correct DMD diagnosis and proper implementation of therapy in patients who do indeed have DMD.
This is the first comprehensive study to describe the prevalence of LGMD2C in one race from mutation study results on patients suspected to have DMD. The incidence of LGMD2C in the Japanese population was estimated to be 1 per 560,000.
We would like to thank Ms. Kanako Yokoyama for her secretarial help. This work was supported by a Grant-in-Aid for Scientific Research (B) and Grant-in-Aid for Exploratory Research from the Japan Society for the Promotion of Science; a Health and Labour Sciences Research Grant for Research on Psychiatric and Neurological Diseases and Mental Health; and a research grant for Nervous and Mental disorders from the Ministry of Health, Labour, and Welfare.
- Emery AEH: Duchenne muscular dystrophy. 1993, Oxford: Oxford University PressGoogle Scholar
- Bonnemann CG, Wong J, Jones KJ, Lidov HG, Feener CA, Shapiro F, Darras BT, Kunkel LM, North KN: Primary gamma-sarcoglycanopathy (LGMD 2C): broadening of the mutational spectrum guided by the immunohistochemical profile. Neuromuscul Disord. 2002, 12: 273-280. 10.1016/S0960-8966(01)00276-0.View ArticlePubMedGoogle Scholar
- Ozawa E, Noguchi S, Mizuno Y, Hagiwara Y, Yoshida M: From dystrophinopathy to sarcoglycanopathy: evolution of a concept of muscular dystrophy. Muscle & Nerve. 1998, 21: 421-438. 10.1002/(SICI)1097-4598(199804)21:4<421::AID-MUS1>3.0.CO;2-B.View ArticleGoogle Scholar
- McNally EM, Duggan D, Gorospe JR, Bonnemann CG, Fanin M, Pegoraro E, Lidov HGW, Noguchi S, Ozawa E, Finkel RS, et al: Mutations that disrupt the carboxyl-terminus of g-sarcoglycan cause muscular dystrophy. Hum Mol Genet. 1996, 5: 1841-1847. 10.1093/hmg/5.11.1841.View ArticlePubMedGoogle Scholar
- Guglieri M, Magri F, D'Angelo MG, Prelle A, Morandi L, Rodolico C, Cagliani R, Mora M, Fortunato F, Bordoni A, et al: Clinical, molecular, and protein correlations in a large sample of genetically diagnosed Italian limb girdle muscular dystrophy patients. Hum Mutat. 2008, 29: 258-266. 10.1002/humu.20642.View ArticlePubMedGoogle Scholar
- Kooi van der AJ, Barth PG, Busch HF, de Haan R, Ginjaar HB, van Essen AJ, van Hooff LJ, Howeler CJ, Jennekens FG, Jongen P, et al: The clinical spectrum of limb girdle muscular dystrophy. A survey in The Netherlands. Brain. 1996, 119: 1471-1480. 10.1093/brain/119.5.1471.View ArticlePubMedGoogle Scholar
- Emery AE: Population frequencies of inherited neuromuscular diseases--a world survey. Neuromuscul Disord. 1991, 1: 19-29. 10.1016/0960-8966(91)90039-U.View ArticlePubMedGoogle Scholar
- Georgieva B, Todorova A, Tournev I, Mitev V, Kremensky I: C283Y gamma-sarcoglycan gene mutation in the Bulgarian Roma (Gypsy) population: prevalence study and carrier screening in a high-risk community. Clin Genet. 2004, 66: 467-472. 10.1111/j.1399-0004.2004.00335.x.View ArticlePubMedGoogle Scholar
- Moore SA, Shilling CJ, Westra S, Wall C, Wicklund MP, Stolle C, Brown CA, Michele DE, Piccolo F, Winder TL, et al: Limb-girdle muscular dystrophy in the United States. J Neuropathol Exp Neurol. 2006, 65: 995-1003. 10.1097/01.jnen.0000235854.77716.6c.View ArticlePubMedGoogle Scholar
- Matsuo M: Duchenne/Becker muscular dystrophy: from molecular diagnosis to gene therapy. Brain Dev. 1996, 18: 167-172. 10.1016/0387-7604(96)00007-1.View ArticlePubMedGoogle Scholar
- Takeshima Y, Yagi M, Wada H, Ishibashi K, Nishiyama A, Kakumoto M, Sakaeda T, Saura R, Okumura K, Matsuo M: Intravenous infusion of an antisense oligonucleotide results in exon skipping in muscle dystrophin mRNA of Duchenne muscular dystrophy. Pediatr Res. 2006, 59: 690-694. 10.1203/01.pdr.0000215047.51278.7c.View ArticlePubMedGoogle Scholar
- Welch EM, Barton ER, Zhuo J, Tomizawa Y, Friesen WJ, Trifills P, Paushkin S, Patel M, Trotta CR, Hwang S, et al: PTC124 targets genetic disorders caused by nonsense mutations. Nature. 2007, 447 (7140): 87-91. 10.1038/nature05756.View ArticlePubMedGoogle Scholar
- Matsuo M, Masumura T, Nishio H, Nakajima T, Kitoh Y, Takumi T, Koga J, Nakamura H: Exon skipping during splicing of dystrophin mRNA precursor due to an intraexon deletion in the dystrophin gene of Duchenne muscular dystrophy Kobe. J Clin Invest. 1991, 87: 2127-2131. 10.1172/JCI115244.View ArticlePubMedPubMed CentralGoogle Scholar
- Tran VK, Zhang Z, Yagi M, Nishiyama A, Habara Y, Takeshima Y, Matsuo M: A novel cryptic exon identified in the 3' region of intron 2 of the human dystrophin gene. J Hum Genet. 2005, 50: 425-433. 10.1007/s10038-005-0272-6.View ArticlePubMedGoogle Scholar
- Yagi M, Takeshima Y, Wada H, Nakamura H, Matsuo M: Two alternative exons can result from activation of the cryptic splice acceptor site deep within intron 2 of the dystrophin gene in a patient with as yet asymptomatic dystrophinopathy. Hum Genet. 2003, 112: 164-170.PubMedGoogle Scholar
- Stec I, Kress W, Meng G, Muller B, Muller CR, Grimm T: Estimate of severe autosomal recessive limb-girdle muscular dystrophy (LGMD2C, LGMD2D) among sporadic muscular dystrophy males: a study of 415 familes. J Med Genet. 1995, 32: 930-933. 10.1136/jmg.32.12.930.View ArticlePubMedPubMed CentralGoogle Scholar
- Vainzof M, Pavanerllo R, Pavanello-Filho I, Rapaport D, Passo-Bueno M, Zubrzycka GEE, Bulman D, Zatz M: Screening of male patients with autosomal recessive Duchenne dystrophy through dystrophin and DNA studies. Am J Med Gent. 1991, 39: 38-41. 10.1002/ajmg.1320390110.View ArticleGoogle Scholar
- Hayashi YK, Mizuno Y, Yoshida M, Nonaka I, Ozawa E, Arahata K: The frequency of patients with 50-kd dystrophin-associated glycoprotein (50DAG or adhalin) deficiency in a muscular dystrophy patient population in Japan: immunocytochemical analysis of 50DAG, 43DAG, dystrophin, and utrophin. Neurology. 1995, 45 (3 Pt 1): 551-554.View ArticlePubMedGoogle Scholar
- Takano A, Bonnemann CG, Honda H, Sakai M, Feener CA, Kunkel LM, Sobue G: Intrafamilial phenotypic variation in limb-girdle muscular dystrophy type 2C with compound heterozygous mutations. Muscle Nerve. 2000, 23: 807-810. 10.1002/(SICI)1097-4598(200005)23:5<807::AID-MUS20>3.0.CO;2-0.View ArticlePubMedGoogle Scholar
- Klinge L, Dekomien G, Aboumousa A, Charlton R, Epplen JT, Barresi R, Bushby K, Straub V: Sarcoglycanopathies: Can muscle immunoanalysis predict the genotype?. Neuromuscul Disord. 2008, 18: 934-941. 10.1016/j.nmd.2008.08.003.View ArticlePubMedGoogle Scholar
- Hack AA, Lam MY, Cordier L, Shoturma DI, Ly CT, Hadhazy MA, Hadhazy MR, Sweeney HL, McNally EM: Differential requirement for individual sarcoglycans and dystrophin in the assembly and function of the dystrophin-glycoprotein complex. J Cell Sci. 2000, 113 (Pt 14): 2535-2544.PubMedGoogle Scholar
- Vainzof M, Passos-Bueno MR, Canovas M, Moreira ES, Pavanello RCM, Marie SK, Anderson LVB, Bonnemann CG, McNally EM, Nigro V, et al: The sarcoglycan complex in the six autosomal recessive limb-girdle muscular dystrophies. Hum Mol Genet. 1996, 5: 1963-1969. 10.1093/hmg/5.12.1963.View ArticlePubMedGoogle Scholar
- Duncan DR, Kang PB, Rabbat JC, Briggs CE, Lidov HG, Darras BT, Kunkel LM: A novel mutation in two families with limb-girdle muscular dystrophy type 2C. Neurology. 2006, 67: 167-169. 10.1212/01.wnl.0000223600.78363.dd.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2350/11/49/prepub
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