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Massive obesity and hyperphagia in posterior bilateral periventricular heterotopias: case report
© Guglielmi et al. 2016
Received: 19 June 2015
Accepted: 1 March 2016
Published: 9 March 2016
Bilateral posterior periventricular nodular heterotopia PNH is a complex malformation of cortical development with imaging features distinguishing it from classic bilateral PNH associated with filamin (FLNA) mutations. It distinctively consists of variably sized nodules of neurons along the trigones and temporal or occipital horns of the lateral ventricles and spectrum of developmental disorders of the mid-/hindbrain. This association suggests that pPNH is part of a more diffuse process of posterior or infrasylvian brain developmental abnormalities other than just a disorder of neuronal migration.
This report describes the first case of an Italian young girl featuring pPNH and severe hyperphagic obesity. At the time of our first examination at age 3 years of age she was severely obese (body mass index, BMI 45.9 Kg/m2) and food-seeking behavior in the free-living situation was reported by the relatives. She showed normal linear growth and cognition, but mildly dysmorphic facial traits including deeply-set eyes, prominent zygomatic bones, downturned mouth corners and low-set ears. Over the years, the patient progressively developed further massive weight gain (at age 9 years, her BMI was 60.4 Kg/m2) and hyperphagia was confirmed by an ad libitum test meal. During follow-up, she presented limitations in walking capacity and in physical functioning due to the disabling obesity. On the basis of distinctive neuro-radiological findings pPNH was diagnosed, in absence of history of seizures.
The present case may contribute to the expansion of the phenotypic expressions of this distinctive complex malformation.
Periventricular nodular heterotopias (PNHs) are common malformations of cortical development consisting of variably sized nodules of neurons along the lateral ventricles, associated with many clinical syndromes and different neuroimaging phenotypes.
Although traditionally PNHs have been considered the result of abnormal neuronal migration [1, 2] recent evidence has suggested that the primary cause may be a disruption of neuroependyma, which impairs postmitotic neurons from attaching to radial glial cells and, therefore, undermines initiation of the migration process [3, 4].
Patients with PNH represent a heterogeneous group from both genetic and phenotypic points of view. The classical bilateral X-linked form is usually caused by mutations of the Filamin A (FLNA) gene  and is characterized by bilateral symmetric heterotopic nodules along the frontal horns and bodies of the lateral ventricles. The clinical spectrum of FLNA mutations is wide and missense mutations or mosaicism may result in unilateral forms or nonlethal expression in males . A rare autosomal recessive form is caused by mutations in the ADP-ribosylation factor guanine nucleotide-exchange factor-2 (ARFGEF2) gene featuring microcephaly and delayed myelination in addition to bilateral PNH . However, associations with several single-gene disorders, chromosomal anomalies  and disruptive causes [9, 10] have been reported, suggesting that heterogeneous genetic and nongenetic processes may cause PNH.
PNH can occur in different patterns and can be associated with different types of malformations. Indeed, they may either be found incidentally in asymptomatic patients, or more frequently discovered after imaging is performed for delayed development or epilepsy [11–13]. Fifteen subtypes have been so far identified on the basis of heterotopic nodules distribution and coexisting birth defects . Bilateral posterior PNH (pPNH), the second most common variant after classic fronto-central PNH, refers to heterotopia exclusively located in the trigones and temporal or occipital horns of the lateral ventricles  and distinctively shows associations with a spectrum of infrasylvian developmental abnormalities.
We describe the case of an Italian girl with severe early-onset obesity. She was second child of healthy, non-consanguineous parents. Gestation was uneventful but prenatal central nervous system ultrasound showed slight dilatation of the right lateral ventricle. Thus, the child was delivered at 38 weeks of pregnancy by Caesarian section and her birth weight and length were 3.17 kg (25–50th centile) and 48 cm (25–50th centile), respectively.
She presented neonatal hypotonia, delayed psychomotor development (she was able to sit without support at 18 months and started walking at 3,5 years) and rapid weight gain (her reported weight at 6 months was 15 Kg) with normal linear growth.
Dysmorphic facial traits including deeply-set eyes, prominent zygomatic bones, downturned mouth corners and mildly low-set ears were also present (Fig. 1a-b). A comprehensive developmental assessment showed normal cognition, communication and motor skills. Ophthalmologic exam documented a mild left convergent strabismus but neither retinopathy, nor eye movement disturbances. Her blood pressure was in the normal range as well as electrocardiographic and echocardiographic evaluations. Radiologic assessment of the skeleton and hands showed no anatomical abnormalities.
The laboratory tests revealed hyperinsulinemia (36 μU/ml) and increased levels of liver enzymes (alanine aminotransferase -ALT- 41 UI/L; aspartate aminotransferase -AST- 73 UI/L; alkaline phosphatase -ALP- 334 UI/L; gamma glutamil transferase -γGT- 82 UI/L). Endocrinological assessment disclosed only a mild increase in adreno-corticotropic hormon (ACTH: 92 pg/ml) with normal serum cortisol levels (13 μg/dl) in line with the degree of obesity. Clinical signs of insulin resistance (acanthosis nigricans of the neck and armpits) and hepatomegaly (also confirmed by abdominal ultrasound scan) were also detected.
She had premature pubertal development and menarche at seven years of age, with post-puberal gonadotropins and sexual hormones levels.
She progressively developed further massive weight gain and, at the time she was nine years old, her BMI, height and weight were 60.4 Kg/m2 (SDS-BMI: 13.6), 145 cm (SDS-height: 2) and 127 Kg (SDS-weight: 23.2) respectively (Fig. 1b). Hyperphagia was confirmed by an 8.2 MJ energy intake in an 18 MJ ad libitum test meal. The degree of hyperphagia (120.6 KJ/Kg lean mass) was comparable to that reported in 50 six-year old patients with heterozygous mutations in the melanocortin 4 receptor gene  and in one patient with congenital deficiency of prohormone convertase 1/3 . Basal metabolic rate measured by indirect calorimetry after an overnight fast was comparable (8.2 MJ/d) to that predicted by age and gender-specific equations (8 MJ/d) .
During an abdominal ultrasound reevaluation, a 25 cm simple ovarian cyst was also detected.
She presented severe impairment of walking capacity and worsening limitations in physical functioning due to her disabling massive obesity. In her primary education, mild intellectual disability appeared as she was perceived by her teacher as having special educational needs, so that she started receiving an inclusive classroom program.
An EEG, performed for the concern of seizures, showed normal findings with mild and diffuse non-specific anomalies.
Genetic testing was performed. The high resolution karyotype analysis and the array comparative genomic hybridisation (CGH) were negative. Neither deletions and duplications of the whole locus, nor point mutations in the coding exons of FLNA gene were detected. Additionally, DNA methylation analysis within the chromosome 15q11.2-q13 excluded the Prader-Willi syndrome.
The first case featuring pPNH and severe hyperphagia/obesity is herein reported.
Patients with pPNH are characterized by nodules typically located along the atria and temporal horns of the lateral ventricles and developmental disorders of the mid-/hindbrain. This association suggests that pPNH is part of a more diffuse process of posterior or infrasylvian brain maldevelopment other than just a disorder of neuronal migration .
Cerebellar signs ranging from severe cerebellar syndrome to mild dysmetria, nystagmus and dysarthria were reported in patients affected by pPNH. Focal epilepsy and normal to mildly impaired cognitive level were also documented [14, 19]. The severe ambulation impairment observed in our patient was not ascribed to cerebellar abnormalities but to the disabling obesity. Besides, our patient did not present either mental retardation or history of seizures.
In keeping with the marked reduction in white matter volume observed in pPNH, some authors hypothesized that this pattern presents greater frequency of connectivity abnormalities . Given also that in Prader-Willi syndrome altered functional connectivity alterations among the brain regions implicated in eating and rewarding have been reported , it would be intriguing to speculate that in pPNH some theoretical neural pathways that control feeding behavior might be disrupted. However, we failed to observe other signs of hypothalamic dysfunction such as temperature instability, high pain threshold, sleep disordered breathing and endocrine abnormalities. Neuro-radiologic alterations of hypothalamus anatomy were excluded as well.
Previous observations have demonstrated that PNH is clinically and genetically heterogeneous and causative genes appear to be scattered through the genome. Whereas the classical bilateral X-linked form is usually caused by mutations of the FLNA gene involved in regulation of cell stability, filopodial protrusion and motility across various biological systems, in contrast, the genetics of pPNH is so far unknown. In fact, the posterior pattern of PNH does not harbour mutations in FLNA. Accordingly, in our patient no FLNA mutations were detected. Thus, pPNH may be considered as a specific malformation complex, which entails a more extensive disturbance in cortical development and putatively reflects aberrations in different mechanisms or pathways to classic PNH. The association of pPNH with mid-/hindbrain malformations may suggest that genes expressed more strongly in the posterior telencephalon might be likely causative candidates  in this pattern of PNH.
The hyperphagic behavior and massive obesity observed in our patient represent a novel clinical feature in pPNH. Although the nosologic classification of pPNH is inherently limited by the lack of genotype-phenotype correlations, and a concomitant monogenic cause (e.g. melanocortin 3 or 4 receptor, leptin and leptin receptor mutations) of the hyperphagic behavior cannot be ruled out, our report reinforces the clinical, and putatively genetic, heterogeneity of pPNH. After all, some of these mutations appear not very likely on the basis of the clinical findings. Namely, disorders in leptin production or action result, beyond severe early-onset obesity, in altered immune function due to T-cell defects and pubertal delay , the latter being in conflict with the premature pubertal development and menarche of our patient. Similarly, children with mutations in the neuroendocrine-specific enzyme prohormone convertase 1/3 present early-onset obesity, reactive hypoglycemia, enteropathy, impaired proopiomelanocortin processing (with consequent decrease in ACTH plasma concentrations) and neuroendocrine dysfunction , features that were not present in our patient. Rather, we found a mild increase in ACTH levels (considered in line with the degree of obesity).
In conclusion, we believe that the present case may contribute to the expansion of the phenotypic expressions of this distinctive complex malformation. However, this case could also represent a new disease entity.
Written informed consent was obtained from the minor child’s mother for publication of this Case report and any accompanying images. A copy of the written consent is available for review by the Editor of this journal. Clinical tests performed and blood sample collection conform to the routine standard care.
We deeply acknowledge Prof. Antonio Cao, distinguished pediatric genetician who recently passed away, for referring the patient. We gratefully acknowledge Dr. Francesco Brancati for the help and useful discussion to delineate the complex phenotype and for the screening of filamin gene. This work was supported by a grant from the Ministero dell’Università e della Ricerca (prot. 2010329EKE_003).
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