Array based characterization of a terminal deletion involving chromosome subband 15q26.2: an emerging syndrome associated with growth retardation, cardiac defects and developmental delay

The subtelomeric regions of chromosomes are particularly gene rich [1] and deletions in these regions have been demonstrated to account for approximately 2.5% of patients displaying mental retardation with/without association of dysmorphic features [2]. The small interstitial deletion on the long arm of chromosome 15 causing Prader-Willi/Angelman (P-W/A) syndrome is well documented [3] whereas cases that report de novo terminal deletions on 15q are rare. Excluding cases displaying various ring structures that result in different sized deletions of chromosome 15 or unbalanced reciprocal translocations involving distal parts of 15q, only 12 cases have been reported harboring pure distal 15q26 deletions [4–14]. These cases display various degrees of clinical features including intrauterine growth retardation, complex cardiac abnormalities, developmental delay, clinodactyly, ear abnormalities, lung hypoplasia, microcephaly, diaphragmatic hernia, high arched palate, and hypoplastic kidneys. Reported survival of patients varied from 20 minutes to 12 years [12].

Previous mapping studies have by means of fluorescent in situ hybridization (FISH) demonstrated that terminal 15q-deletion frequently results in loss of one IGF1R allele. It has been postulated that a dosage effect of the IGF1R gene might be direct responsible for the intrauterine growth retardation seen in patients monosomic for 15q26 and for the postnatal overgrowth seen in patients trisomic for 15q25-qter [15]. However in vitro studies on fibroblast cells have been ambiguous. For instance Siebler et al. (1999) failed to provide conclusive evidence for a biological response to lowered IGFR1 expression due to distal deletion of 15q whereas Okubo et al. demonstrated accelerated and decreased growth in cells with three or one copy of the gene, respectively [5, 8].

In this report we present a detailed genetic mapping including classical cytogenetics, metaphase FISH and tiling resolution (32 k) genome-wide array-based comparative genomic hybridization (array CGH) of a patient with a de novo deletion of subband 15q26.2, caused by the formation of a dicentric chromosome 15. Only one similar case with a karyotype harboring a bisatellite dicentric chromosome, resulting in a distal 15q deletion, has been reported previously in the literature [6].

The patient is the first child to healthy parents (maternal age 29 years) and was born at the 37^th week of pregnancy. APGAR score was 8-9-9. The birth-weight was 2.4 kg, borderline to small for gestational age. Single umbilical artery was not present. Growth and height centiles were ≤3 for the patient both at 1, 18 and 22 months of age. The child was first examined in the 33^rd gestational week, when fetal screening had indicated abnormal heart size. Fetal echocardiography showed a clearly enlarged right ventricle and a narrow left ventricle with a slightly small mitral valve. There was suspicion of a ventricular septal defect (VSD). The aortic arch could not be completely elucidated. A magnetic resonance tomography (MRT) was made of the fetus, but no further information was obtained. Neonatal echocardiography confirmed a large right ventricle, a somewhat underdeveloped left side, and gave a strong suspicion of coarctation of the aorta. There was also a small VSD. Treatment with prostaglandin was started and the child was put on ventilator. MRT showed a coarctation and an abnormal aortic arch with a very acute angle between the ascending and the descending aorta. The child was operated at the age of three days with resection of the coarctation and end-to-side anastomosis. The postoperative period was uneventful except for bouts of supraventricular tachycardia that were treated with adenosine and digitalis. X-ray showed relaxation of the right side of the diaphragm. Because of the patient's unusual facial features with low set ears, small chin, and haemangioma on the forehead, a chromosome analysis was performed on a blood sample drawn at day five.

At the age of 18 months the child is small with a weight of 7,7 kg and a length of 70 cm. The patient's physical and mental developmental milestones are severely delayed. He has been operated with a percutaneous gastrostomy and takes hardly any food by mouth, harboring problems with vomiting and gastrooesophageal reflux. At the lastest follow up the patients cardiac status was fairly good, indicating favorable result of the coarctation operation, but displayed signs of a moderate mitral stenosis and a remaining small VSD. He displays dysmorphic features like hypertelorism, rocker bottom feet, broad nasal bridge, narrow upper lip and flat philtrum (Figure 1).

Blood samples from the patient and his parents were drawn after informed consent. Lymphocyte culture upon phytohemagglutinin stimulation and G-banding analysis was performed in standard clinical settings on the three samples. Both parents displayed normal karyotypes.

DNA was extracted from the peripheral blood cells obtained at the time of diagnosis. Male genomic DNA (Promega, Madison, WI) was used as reference in the hybridization. The 32 k slides used, containing 32 433 tiling BAC clones covering at least 98% of the human genome, were produced at the SWEGENE DNA microarray resource center at Lund University, Sweden. Labeling of DNA, slide preparation, and hybridization were performed as described [17] with minor modifications. Analysis of the microarray images were performed with the GenePix Pro 4.0 software (Axon Instruments, Foster City, CA). For each spot, the median pixel intensity minus the median local background for both dyes was used to obtain the ratio of test gene copy number to reference gene copy number. Data normalization was performed for each array subgrid using lowess curve fitting with a smoothing factor of 0.33 [18]. The sex chromosomes were excluded when calculating the correction factor in the normalization of the data set. All normalizations and analyses were performed in the Bioarray Software Environment database (BASE) [19]. To identify imbalances, the MATLAB toolbox CGH plotter and the TM4 software suite [20] was applied, using a moving mean average over three clones and log2 limits of ≥0.2. Classification as gain or loss was based on identification as such by the CGH plotter and also by visual inspection of the log2 ratios. Ratios +/- ≥0.5 in three adjacent clones were classified as abnormal, with ratios between 0.5 and 1.0 interpreted as duplications/hemizygous deletions and ≥1.0 classified as amplifications/homozygous deletions.

Written informed consent was obtained for publication of this study.

Constitutional deletions involving chromosome band 15q26 seem to be rather rare unbalanced aberrations which seldom are diagnosed and characterized at the cytogenetic level. However with subtelomeric FISH becoming standard clinical practice and array CGH being more commonly implicated as a powerful tool to characterize cases with submicroscopic aberrations it is likely to assume that patients harboring distal 15q-deletions might be more frequently discovered in the future.

In this study we used array CGH to delineate the breakpoint to position 93.86 Mb on chromosome 15. This was consistent with the preceding FISH analysis that localized the most proximally deleted BAC as clone RP11-79C10, positioned within subband 15q26.2, and encompassing the area detected by array CGH [21]. This approach of using tiling BACs to cover the whole genome in a single hybridization gives a resolution standard of 150–350 kb, applying the exclusion criteria that three contiguous clones must display abnormal log2 ratios to be considered to signal an aberration. This study, characterizing in detail a constitutional 15q26-deletion, is one of the first ones using molecular cytogenetic characterization with submegabase resolution on a syndrome related to this chromosomal region. Klaassens et al. (2005) used a similar approach relying on array CGH and FISH, but focused on cases presenting with distal 15q breakpoints resulting from ring chromosome formation or unbalanced translocations to determine a critical region associated with congenital diaphragmatic hernia (CDH). The platform used was a 1-Mb Human BAC array, giving resolution standards tenfold smaller than 32 k arrays [11]. However, a later study used array techniques with submegabase resolution on a similar patient [22]. In addition Slavotinek et al. (2005) have previously identified microdeletions at 15q26.2 to be associated with Fryns syndrome, a CDH-related autosomal recessive syndrome with relatively homogenous phenotype, using 2.4 k array [14].

The origin of the dicentric chromosome in the present case remains unknown since both parents displayed normal karyotypes and directed microsatellite analysis was not performed. However, it has been demonstrated that a de novo 15q-deletion can be of both paternal and maternal origin [4, 6]. Studies on terminal chromosomal deletions have suggested that de novo telomere addition, by telomerase activity or recombinant mechanisms, work to stabilize the affected chromosome [23]. It could be speculated that the latter event might explain the formation of the dic(15) in the present case, but involving centromeres rather than telomeres. It is noteworthy that no duplication of near-centromeric q-arm material could be detected on the log2 ratio plot of chromosome 15 (Figure 4). The preceding FISH analysis had confirmed that the duplication included the centromeric region, as well as p-arm material, both being located in regions not covered by the array.

The vast majority of patients with deletions of distal 15q have displayed pre- and postnatal growth retardation, cardiac defects, developmental delay, ear abnormalities, and clinodactyly. A variety of other different dysmorphic features have however been reported in association with 15q26 deletions such as café-au-lait spots, cholestatic liver disease, diaphragmatic hernia, eye and face abnormalities, equinovarus, high arched palate, hypertelorism, hypoplastic kidneys, hypotonia, lung hypoplasia, microcephaly, micrognathia, and spinal cord abnormality. Although high resolution cytogenetic mapping for all reported cases are lacking this wide range of phenotypic manifestations probably reflect size heterogeneity of distal 15q-deletions. More precise data on 15q-breakpoints might enable an elucidation of different candidate regions for different phenotypic manifestations. For instance, patients suffering from CDH in association with 15q26-deletion have been extensively studied and a critical region associated with CDH was assessed to a region ranging from distal 15q26.1 to proximal 15q26.2 [11]. The 15q-breakpoint in the present case mapped outside of the suggested critical region and in accordance did not suffer from CDH. Deletions of 15q26 have also been reported in association with Russel-Silver syndrome, which share many of the clinical features displayed in patients with deletions of distal 15q and it is possible that these cases should be categorized as an subgroup of the latter syndrome [24, 25].

Considering all the reported cases with deletion of 15q26, the salient finding must be that the majority report loss of IGFR1, a gene that contributes to a wide range of developmental processes [26]. The relation between IGFR1 and pre- and postnatal growth in association with distal 15q-deletions has been studied without conclusive results [5, 8]. In relation to the developmental delay commonly seen in patients with distal 15q-deletions, the IGF1 receptor gene plays an important function in the development of the central nervous system, which could partly explain this feature [27]. Studies on the effect of IGFR1 on the cardiovascular system indicate that it is an important player in heart development [24]. Monozygosity of this gene could therefore partly contribute to the complex cardiac defects so often seen in patients with this deletion. Obviously, all suggested clinical features postulated to relate to loss of one copy of the IGFR gene remains to be elucidated, preferentially by further gene expression studies on diagnostic material.

MEF2A have been suggested as a candidate gene for the cardiac defects [11] included in the phenotype of 15q26-deletions since the gene encode for DNA-binding regulatory protein that enhances the differentiation of mesodermal precursor cells to myoblast and therefore is key player in cardiac myocyte development [28]. The MEF2-family is an ancient mediator of signal-dependant transcription and diverse developmental programs and MEF2A was quite recently demonstrated to be involved in morphogenesis of postsynaptic neurons [29] which implicate that it could also contribute to the developmental delay seen in the patient.

Another gene in the deleted segment that could be directly implicated due to its protein function is CHSY1 that encodes chondroitin sulfate [30], a glycosaminoglycan expressed on the surface of most cells and in extracellular matrices. Glycosaminoglycan chains are covalently linked to a wide range of core protein families and regulate many biologic processes, including cell proliferation and recognition, extracellular matrix deposition, and morphogenesis. Furthermore, TM2D3, located in the deleted region, synthesizes a protein that have a structural module related to that of the seven transmembrane domain G protein-coupled receptor superfamily and may have regulatory roles in cell death or proliferation signal cascades [31].

We present a case with a dic(15;15)(q11;q26.1) which was thoroughly characterized with locus specific FISH and tiling resolution array CGH. The dicentric chromosome harbor a terminal deletion involving 15q26.2-qter a region that encompass 14 genes, among which four candidate genes could be delineated. MEF2A has previously been suggested as a candidate gene for the cardiac defects seen in investigated patients. In addition CHSY1 is involved in cell proliferation and morphogenesis and TM2D3 may have regulatory roles in cell death or proliferation signal cascades. Previous studies have demonstrated that terminal 15q-deletions frequently results in loss of one allele of the IGF1R gene, a finding also observed in our patient. IGF1R contributes to a wide range of developmental processes such as development of the central nervous and cardiovascular systems, and monozygosity for this gene could contribute to the developmental delay and complex cardiac defects seen in our patient. However, all suggested clinical features postulated to relate to IGF1R deletions remains to be elucidated, preferentially by gene expression studies on patients harboring similar 15q-aberrations. Hopefully, this may the future lead to the description of a clear cut clinically recognizable syndrome.