- Research article
- Open Access
Systematic analysis of mitochondrial genes associated with hearing loss in the Japanese population: dHPLC reveals a new candidate mutation
BMC Medical Genetics volume 12, Article number: 135 (2011)
Variants of mitochondrial DNA (mtDNA) have been evaluated for their association with hearing loss. Although ethnic background affects the spectrum of mtDNA variants, systematic mutational analysis of mtDNA in Japanese patients with hearing loss has not been reported.
Using denaturing high-performance liquid chromatography combined with direct sequencing and cloning-sequencing, Japanese patients with prelingual (N = 54) or postlingual (N = 80) sensorineural hearing loss not having pathogenic mutations of m.1555A > G and m.3243A > G nor GJB2 were subjected to mutational analysis of mtDNA genes (12S rRNA, tRNA Leu(UUR), tRNA Ser(UCN), tRNA Lys, tRNA His, tRNA Ser(AGY), and tRNA Glu).
We discovered 15 variants in 12S rRNA and one homoplasmic m.7501A > G variant in tRNA Ser(UCN); no variants were detected in the other genes. Two criteria, namely the low frequency in the controls and the high conservation among animals, selected the m.904C > T and the m.1105T > C variants in 12S rRNA as candidate pathogenic mutations. Alterations in the secondary structures of the two variant transcripts as well as that of m.7501A > G in tRNA Ser(UCN)were predicted.
The m.904C > T variant was found to be a new candidate mutation associated with hearing loss. The m.1105T > C variant is unlikely to be pathogenic. The pathogenicity of the homoplasmic m.7501T > A variant awaits further study.
Hearing loss manifests in more than 1 in 1000 persons at birth, and the frequency increases subsequently to 3 in 1000 by 4 years of age [1, 2]. Approximately 50 to 70% of congenital and childhood deafness is estimated to be due to genetic mutations. In adults, the prevalence of hereditary hearing impairment has been estimated to be approximately 3.2 in 1000 . Some of the mitochondrial DNA (mtDNA) genes, such as 12S rRNA, tRNA Leu(UUR), and tRNA Ser(UCN), are known to be responsible for hereditary hearing loss . Among them, the m.1555A > G mutation in 12S rRNA is found relatively frequently (0.6-16%, depending on the ethnic group) in aminoglycoside-induced, congenital, and late-onset nonsyndromic hearing loss [4, 5]. The m.1494C > T mutation in 12S rRNA is also associated with aminoglycoside-induced and nonsyndromic hearing loss [6, 7]. The m.3243A > G mutation in tRNA Leu(UUR)is associated with late-onset nonsyndromic hearing loss [8, 9], maternally inherited diabetes and deafness (MIDD) [10, 11], and mitochondrial myopathy, encephalopathy, lactic acidosis, stroke-like episodes (MELAS), which frequently presents with hearing loss [12, 13]. The m.7445A > C/G/T [14–16], 7472insC , and 7510T > C mutations  in tRNA Ser(UCN)are also associated with aminoglycoside-induced, nonsyndromic, or syndromic hearing loss.
In addition, many other variants in 12S rRNA have been proposed to be associated with hearing loss . Some variants such as m.827A > G [19, 20], 961T > C , 961delT + Cn [21, 22], 1005T > C , and 1095T > C in 12S rRNA [22–26] are not definitively related to hearing loss, because they have been found in subjects with normal hearing and/or are not conserved among mammals [19, 27–30]. Moreover, a variety of mitochondrial haplogroups often localize in specific ethnic groups, making it difficult to determine whether the mtDNA variants are associated directly with diseases, indirectly as risk factors, or simply with rare subhaplogroups [31–34]. Accumulating reports of various novel mtDNA mutations associated with hearing loss prompted us to evaluate these variants in patients with hearing loss in Japan, where mtDNA mutation studies have focused on a few limited nucleotide positions [35, 36].
A single cell contains hundreds of mitochondria, and the mtDNA in each mitochondrion is occasionally heterogeneous, a feature called heteroplasmy . The proportion of pathogenic mutations of heteroplasmic mtDNA is considered to be one of the reasons for the wide range of severity of phenotypes seen in patients with mitochondrial-related diseases, such as those reported in the case of the m.3243A > G mutation [38–40]. Denaturing high-performance liquid chromatography (dHPLC) is a sensitive method to detect heteroplasmic mutations that can be overlooked by simple direct sequencing and comparison of the scanned peaks or restriction fragment length polymorphism-PCR [28, 41]. In this study, we conducted a systematic mutational analysis of mtDNA by dHPLC combined with direct sequencing and cloning-sequencing in samples from Japanese patients with hearing loss.
Subjects with bilateral sensorineural hearing loss were recruited by the National Tokyo Medical Center and collaborating hospitals. Subjects' medical histories were obtained and physical examinations were performed to exclude those subjects with syndromic symptoms, diseases of the outer or/and middle ear, and environmental factors related to hearing loss such as history of infectious diseases, premature birth, and newborn meningitis. Patients with a history of use of ototoxic drugs were included in the study because these drugs are known to be associated with mitochondrial hearing loss. Prior to this study, the patients were confirmed not to have the m.1555A > G and m.3243A > G mutations or not to be diagnosed as having GJB2 -caused hearing loss, as assessed by restriction fragment length polymorphism-PCR or together with direct sequencing if the heterozygotic 235delC mutation was detected in GJB2 [42, 43]. The 134 subjects were classified into prelingual hearing loss (onset before 5 years old, 20 males and 34 females) or postlingual hearing loss (onset at 5 years old or later, 31 males and 49 females) . The control group consisted of 137 unrelated Japanese individuals with normal hearing as examined by pure-tone audiometry. All subjects or their parents gave prior informed consent for participation in this study. This study was approved by the ethics committee of National Tokyo Medical Center.
Screening for mtDNA mutations by dHPLC
DNA was extracted from blood samples using the Gentra Puregene DNA isolation kit (QIAGEN, Hamburg, Germany). Initially, whole mtDNA from each patient was amplified in three overlapping fragments (1351-8197, 6058-12770, and 11706-2258)  by LATaq DNA polymerase (TaKaRa BIO, Shiga, Japan). PCR was conducted at 94°C for 1 min followed by 30 cycles of 98°C for 10 s and 68°C for 6.5 min. Then, using the PCR products as templates, variants were analyzed by the Mitoscreen assay kit (Transgenomic, Glasgow, UK). We amplified the genes 12S rRNA, tRNA Leu(UUR), tRNA Ser(UCN), tRNA Lys, tRNA His, tRNA Ser(AGY), and tRNA Glu, for which mutations were reported to be associated with hearing loss on the Hereditary Hearing Loss Homepage  when the study was started. The PCR products using primer sets MT4 (for 12S rRNA), MT6 (tRNA Leu(UUR)), MT10 (tRNA Ser(UCN)), MT11 (tRNA Lys), MT15 (tRNA Hisand tRNA Ser(AGY)), and MT18 (tRNA Glu) were incubated with the appropriate restriction enzymes, incubated for heteroduplex formation either with reference PCR products to detect homoplasmy or with their own PCR products to detect heteroplasmy, then analyzed by dHPLC (WAVE system, Transgenomic) according to the manufacturer's protocols.
The reference mtDNA was derived from a Japanese individual with normal hearing. Sequencing of the entire reference mtDNA revealed 750A > G and 1438A > G polymorphisms, and the mtDNA sequence was otherwise comparable to the revised Cambridge Reference sequence (AC_000021) [46, 47]).
When homoplasmic or heteroplasmic variants were detected, the PCR product was subjected to direct sequencing by the BigDye Terminator ver. 3 cycle sequencing kit and ABI genetic analyzer 3730 (Life Technologies, Carlsbad, CA). To sequence 12S rRNA, an additional nested PCR product (656-1,266) was amplified with primers F (5'-tggtcctagcctttctattagctctt-3') and R (5'-tggcggtatataggctgagca-3'). To sequence tRNA Ser(UCN), an additional nested PCR product (7,209-7,609) was amplified with primers F (5'-atgccccgacgttactcg-3') and R (5'- acctacttgcgctgcatgtg-3'). To determine the proportion of heteroplasmic 1005T > C variant in the 12S rRNA, the nested PCR (656-1,266) product was cloned and sequenced. Nested PCR was carried out by replacing AmpliTaq Gold DNA polymerase with PrimeSTAR DNA polymerase, which has 3'-proofreading activity (TaKaRa BIO), followed by the Zero Blunt TOPO PCR cloning kit (Life Technologies). We sequenced 54 clones derived from the proband mtDNA and 24 clones derived from the mtDNA of each of five siblings. Sequencing data were analyzed by SeqScape ver2.6 (Life Technologies) and DNASIS Pro (Hitachisoft, Tokyo, Japan). The sequencing results for each patient were compared with the revised Cambridge Reference sequence to identify mtDNA variants. The uniqueness of each mutation was evaluated by comparison with the mtSNP database , MITOMAP , and the Uppsala mtDB database .
Prediction of pathogenicity of mtDNA variants
The variants were evaluated based on double selection as proposed by Leveque and coworkers , with modification. Initially, we measured the frequencies of each variant found in the controls in our study (N = 137) and in the mtSNP database (N = 672, including: centenarians in Gifu, centenarians in Tokyo, type 2 diabetes mellitus patients (without or with vascular disorders), overweight young adult males, non-overweight young adult males, Parkinson's disease patients, and Alzheimer's disease patients in Japan). The variants with a frequency of more than 3% in one of the groups were considered as non-pathologic polymorphisms. We used a frequency threshold lower than that previously used (4%)  because the mtSNP database of Japanese individuals and the controls reflect the patient ethnic group background more closely than the mtDB and therefore requires a lower frequency threshold to exclude polymorphisms. The nucleotide conservation in each gene from human and 50 mammalian species was evaluated by ClustalW. The additional file lists the mammalian species and the accession numbers of the mtDNA (Additional File 1: Table S1). The variant frequencies in the mtDB were calculated to determine if the low variant frequencies measured in the controls reflect rare haplotypes in the Japanese population and are more common worldwide. All the variants were also analyzed with PhyloTree (mtDNA tree Build 10)  to search for previously characterized variants in haplogroups. Pathogenicity of the variants was also evaluated by predicting the secondary structures of the mitochondrial transcripts with or without the variant using Centroid Fold [53, 54].
dHPLC screening and subsequent direct sequencing in the patients identified 12 homoplasmic or heteroplasmic variants in 12S rRNA and 1 homoplasmic variant in tRNA Ser(UCN)(Table 1). In addition, the 3 homoplasmic variants, m.752C > T, 1009C > T, and 1107T > C in 12S rRNA were detected in the controls by direct sequencing. All the patients and the controls appeared to have the non-pathogenic m.750A > G and 1438A > G variants, as previously noted . No tRNA Glu, tRNA Leu(UUR), tRNA Lys, tRNA His, or tRNA Ser(AGY)variants were detected. Table 1 lists the number of patients found with each variant, the frequencies of the variants in the controls and among Japanese individuals with various clinical conditions (mtSNP, N = 672), previous reports of the variants, and the frequencies of the variants in the mtDB. We evaluated two criteria, namely that the frequency of the variants be < 3% in both the controls and in the Japanese database (mtSNP) and that the variant nucleotide conserved by >50% among the 51 mammalian species we considered ; based on this analysis, two 12S rRNA variants, m.904C > T and 1005T > C, were selected as candidate pathogenic mutations and subjected to further study. Although the homoplasmic m.7501T > A variant in tRNA Ser(UCN)did not meet the conservation criteria, it was also subjected to further study because several other tRNA Ser(UCN)mutations have been reported to be associated with hearing loss, whereas the m.7501T > A variant has not been studied for its pathogenicity.
A novel homoplasmic m.904C > T variant in the12S rRNA was found in a 46-year-old female patient (Figure 1A). She did not possess additional mtDNA pathogenic mutations and showed prelingual, progressive hearing loss with tinnitus. The patient was suspected of hearing impairment as early as 4 years old and was diagnosed with sensorineural hearing loss at age 11. The audiometric examination showed mild hearing loss at low frequencies and no response at 1 kHz and higher frequencies (Figure 1B). She had no response to an otoacoustic emission test, indicating dysfunction of the auditory outer hair cells. The patient had no history of treatment with ototoxic drugs and did not suffer from any other symptoms. The siblings also suffered from prelingual, severe hearing loss (with similar ages of onset and severity), but their parents had normal hearing (Figure 1A). The patient bore two children with normal hearing. DNA samples were not obtained from other family members. The secondary structure of the variant 12S rRNA predicted by Centroid Fold suggested that substitution of C > T (transcribed as U) at position 904 of the 12S rRNA results in gross structural alteration of the transcript region that includes nucleotide positions 862 to 917, in addition to truncation of the stem-like structure from positions 1021 to 1030 (Figure 1C and 1D), implicating a significant role for 904C in 12S rRNA folding.
The homoplasmic m.1005T > C variant in the 12S rRNA was found in a male patient with prelingual, severe hearing loss (Figure 2A, B). The patient's spouse had prelingual hearing loss owing to measles, and their child also had prelingual hearing loss. The m.1005T > C variant was not detected in the patient's spouse or daughter. DNA samples were not obtained from other family members.
The heteroplasmic m.1005T > C variant together with the homoplasmic mutation m.709G > A was detected in a male patient from a consanguineous marriage of parents with normal hearing (Figure 2C). In the proband (III:3), onset of hearing loss and diabetes mellitus occurred in his 40s. Among his five siblings, four (III:1, 2, 4, 6) also showed adult-onset hearing loss between age 20 and 50 years, but they did not have diabetes mellitus. The fifth sibling suffered from infantile paralysis and died at age 6 (III:5). Cloning of the fragment of 12S rRNA, which demonstrated apparent heteroduplex formation (Figure 2D, arrow), yielded 12 of 54 clones (22%) with the m.1005T > C variant. However, the m.1005T > C variant was not detected in 24 clones derived from the mtDNA from each of these siblings, indicating that the variant was absent in the siblings or the frequency was less than 4%. The audiograms showed severe to profound hearing loss in the siblings III:1, 2, 3, and 4 (Figure 2E, F, 3A, B). The secondary structure of the 12S rRNA variant predicted by Centroid Fold indicated that the m.1005T > C induces a gross structural alteration in the transcript, including nucleotide positions 862 to 917 (Figure 1C and 3C).
Three patients appeared to carry the homoplasmic m.7501T > A variant in tRNA Ser(UCN)(Figure 4A, C, E). One female patient suffered from episodic vertigo from age 27 years followed by tinnitus and fluctuant, moderate progressive hearing loss, and she had no familial history of hearing loss (Figure 4A, B). Another female patient suffered from tinnitus beginning at age 24 years and had been exposed to streptomycin from age 36 to 37 for treatment of tuberculosis (Figure 4C, D). She suffered from fluctuant, moderate hearing loss from her 50s and had no familial history of hearing loss. The third patient was a male from a consanguineous marriage of parents with normal hearing and showed non-progressive, severe hearing loss from childhood without tinnitus or vertigo (Figure 4E, F). Later, he was also found to have X-linked spinal and bulbar muscular atrophy (SBMA/Kennedy-Alter-Sung disease/Kennedy's disease). In this family, six of seven siblings showed hearing loss. Family members other than the proband did not participate in this study. According to the secondary structure prediction by Centroid Fold, the m.7501T > A in tRNA Ser(UCN)(which is transcribed as U in the reverse direction) causes an elongation of the D-arm in the transcript by reducing the size of the D-loop of tRNASer(UCN) (Figure 4G, H), which might affect biosynthesis of mitochondrial proteins .
In our study, screening of mtDNA by dHPLC and direct sequencing detected 15 variants in 12S rRNA and 1 variant in tRNA Ser(UCN). Comparison of the variant frequencies in controls, assessment of nucleotide conservation among mammalian species, and structural analysis of the transcript was used to select candidate mutations associated with hearing loss. No variants in tRNA Leu(UUR), tRNA Lys, tRNA His, tRNA Ser(AGY), or tRNA Gluwere detected in the subjects studied here, suggesting that the mutations in these genes associated with hearing loss are not common in the Japanese population.
To our knowledge, the homoplasmic m.904C > T variant in 12S rRNA has not been reported elsewhere. Lack of symptoms in the maternal relatives does not exclude mitochondrial transmission, because penetrance of 12S rRNA mutations can be extremely low, as seen in the m.1555A > G associated with hearing loss . Conservation of the nucleotides among mammals and gross alteration of the predicted secondary structure of the 12S rRNA transcript suggest that the m.904C > T variant might affect auditory function by changing the efficiency with which mRNAs are transcribed to yield mitochondrial proteins.
A patient with the homoplasmic m.1005T > C variant in the 12S rRNA had a child with prelingual hearing loss. The inheritance of hearing loss in the child is likely due to the transmission of an autosomal mutation, not mtDNA, from the male proband. Therefore, the data for this family may not provide unequivocal information about the pathogenicity of the m.1005T > C variant [4, 22, 27, 30].
Identification of the heteroplasmic m.1005T > C variant in a patient with hearing loss is a novel finding, because this variant has been known only as homoplasmic [22, 27, 30, 34]. We did not verify that the heteroplasmic m.1005T > C variant was correlated with hearing loss because four of five siblings of the proband had hearing loss without carrying the variant, whereas it might be associated with diabetes mellitus. However, it is difficult to exclude the possibility of association of the heteroplasmic variant detected in blood samples with mitochondrial diseases such as deafness. Frequencies of heteroplasmy of mtDNA vary considerably among tissues in the same individual (for instance, [37, 57, 58]). Therefore, it is possible that the frequency of the m.1005T > C variant in the inner ear cells of the siblings is much higher than in the blood cells and thus may underlie the hearing loss.
Another finding in this study is that three patients with postlingual hearing loss had the homoplasmic m.7501T > A variant in tRNA Ser (UCN). Various mutations in tRNA Ser(UCN), such as m.7445A > G [15, 16], 7472insC [17, 59], 7505T > C , 7510T > C , and 7511T > C [51, 59, 61], are associated with various types of hearing loss (syndromic or nonsyndromic, prelingual or late-onset), raising the possibility that the m.7501T > A variant, reported elsewhere without detailed investigation , is also associated with hearing loss. The low conservation of the variation at this position (29% among mammals) does not support the pathogenicity of the variant, in contrast to the much higher conservation at m.7472A (61%), 7505A (98%), 7510T (78%), and 7511T (98%). On the other hand, the m.7501T > A variant is predicted to modify the secondary structure of the D-arm in the tRNA Ser(UCN)transcript; the D-arm is important for the stability of the transcript and the general rate of mitochondrial protein synthesis . Further investigation, such as haplogroup analysis or generating lymphoblastoid cell lines to measure endogenous respiration rates, may help to define the pathogenicity of the m.7501T > A variant.
All other variants found in this study, such as m.827A > G, 961insC, and 961delT + Cn, which have been discussed elsewhere with respect to their pathogenicity [21, 22, 27, 30, 62], were considered to be non-pathologic polymorphisms because they were found frequently in the controls. The other variants, m.663A > G, 709G > A, 750A > G, 752C > T, 1009C > T, 1041A > G, 1107T > C, 1119T > C, 1382A > C, and 1438A > G, were frequently detected in the controls and considered to be nonpathogenic polymorphisms, which is in consistent with a previous report . The spectrum of variants of mitochondrial genes in Japanese individuals was similar to that in a Chinese population , for which most of the variants detected in this study (other than the m.904C > T and 7501T > A) have been reported. In contrast, the spectrum was dissimilar to those in other ethnic groups such as the Polish population [19, 63]. Our results indicate that ethnic background should be taken into consideration when studying the pathogenicity of mtDNA variants based on their frequencies in controls.
We sought to detect mitochondrial variants other than m.1555A > G or 3243A > G mutations, which are known to be related to hearing loss, by dHPLC, direct sequencing, and cloning-sequencing in samples from Japanese patients with hearing loss. The homoplasmic m.904C > T variant in 12S rRNA was considered to be a new candidate mutation associated with hearing loss. The pathogenicity of the m.7501T > A variant in tRNA Ser(UCN)remains inconclusive, and other variants identified in this study, including the heteroplasmic m.1005T > C variant, are not positively associated with hearing loss. No variants were detected in the in tRNA Leu(UUR), tRNA Lys, tRNA His, tRNA Ser(AGY), and tRNA Glu.
Morton CC, Nance WE: Newborn hearing screening--a silent revolution. N Engl J Med. 2006, 354: 2151-2164. 10.1056/NEJMra050700.
Kral A, O'Donoghue GM: Profound deafness in childhood. N Engl J Med. 2010, 363: 1438-1450. 10.1056/NEJMra0911225.
Sakihara Y, Christensen B, Parving A: Prevalence of hereditary hearing impairment in adults. Scand Audiol. 1999, 28: 39-46. 10.1080/010503999424897.
Kokotas H, Petersen MB, Willems PJ: Mitochondrial deafness. Clin Genet. 2007, 71: 379-391. 10.1111/j.1399-0004.2007.00800.x.
del Castillo FJ, Rodriguez-Ballesteros M, Martin Y, Arellano B, Gallo-Teran J, Morales-Angulo C, Ramirez-Camacho R, Cruz Tapia M, Solanellas J, Martinez-Conde A, et al: Heteroplasmy for the 1555A > G mutation in the mitochondrial 12S rRNA gene in six Spanish families with non-syndromic hearing loss. J Med Genet. 2003, 40: 632-636. 10.1136/jmg.40.8.632.
Chen J, Yang L, Yang A, Zhu Y, Zhao J, Sun D, Tao Z, Tang X, Wang J, Wang X, et al: Maternally inherited aminoglycoside-induced and nonsyndromic hearing loss is associated with the 12S rRNA C1494T mutation in three Han Chinese pedigrees. Gene. 2007, 401: 4-11. 10.1016/j.gene.2007.06.009.
Zhao H, Li R, Wang Q, Yan Q, Deng JH, Han D, Bai Y, Young WY, Guan MX: Maternally inherited aminoglycoside-induced and nonsyndromic deafness is associated with the novel C1494T mutation in the mitochondrial 12S rRNA gene in a large Chinese family. Am J Hum Genet. 2004, 74: 139-152. 10.1086/381133.
Chinnery PF, Elliott C, Green GR, Rees A, Coulthard A, Turnbull DM, Griffiths TD: The spectrum of hearing loss due to mitochondrial DNA defects. Brain. 2000, 123 (Pt 1): 82-92.
Deschauer M, Muller T, Wieser T, Schulte-Mattler W, Kornhuber M, Zierz S: Hearing impairment is common in various phenotypes of the mitochondrial DNA A3243G mutation. Arch Neurol. 2001, 58: 1885-1888. 10.1001/archneur.58.11.1885.
van den Ouweland JM, Lemkes HH, Ruitenbeek W, Sandkuijl LA, de Vijlder MF, Struyvenberg PA, van de Kamp JJ, Maassen JA: Mutation in mitochondrial tRNA(Leu)(UUR) gene in a large pedigree with maternally transmitted type II diabetes mellitus and deafness. Nat Genet. 1992, 1: 368-371. 10.1038/ng0892-368.
Maassen JA, Jahangir Tafrechi RS, Janssen GM, Raap AK, Lemkes HH, t Hart LM: New insights in the molecular pathogenesis of the maternally inherited diabetes and deafness syndrome. Endocrinol Metab Clin North Am. 2006, 35: 385-396. 10.1016/j.ecl.2006.02.014.
Goto Y, Nonaka I, Horai S: A mutation in the tRNA(Leu)(UUR) gene associated with the MELAS subgroup of mitochondrial encephalomyopathies. Nature. 1990, 348: 651-653. 10.1038/348651a0.
Kobayashi Y, Momoi MY, Tominaga K, Momoi T, Nihei K, Yanagisawa M, Kagawa Y, Ohta S: A point mutation in the mitochondrial tRNA(Leu)(UUR) gene in MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes). Biochem Biophys Res Commun. 1990, 173: 816-822. 10.1016/S0006-291X(05)80860-5.
Jin L, Yang A, Zhu Y, Zhao J, Wang X, Yang L, Sun D, Tao Z, Tsushima A, Wu G, et al: Mitochondrial tRNASer(UCN) gene is the hot spot for mutations associated with aminoglycoside-induced and non-syndromic hearing loss. Biochem Biophys Res Commun. 2007, 361: 133-139. 10.1016/j.bbrc.2007.06.171.
Reid FM, Vernham GA, Jacobs HT: A novel mitochondrial point mutation in a maternal pedigree with sensorineural deafness. Hum Mutat. 1994, 3: 243-247. 10.1002/humu.1380030311.
Maasz A, Komlosi K, Hadzsiev K, Szabo Z, Willems PJ, Gerlinger I, Kosztolanyi G, Mehes K, Melegh B: Phenotypic variants of the deafness-associated mitochondrial DNA A7445G mutation. Curr Med Chem. 2008, 15: 1257-1262. 10.2174/092986708784534910.
Verhoeven K, Ensink RJ, Tiranti V, Huygen PL, Johnson DF, Schatteman I, Van Laer L, Verstreken M, Van de Heyning P, Fischel-Ghodsian N, et al: Hearing impairment and neurological dysfunction associated with a mutation in the mitochondrial tRNASer(UCN) gene. Eur J Hum Genet. 1999, 7: 45-51. 10.1038/sj.ejhg.5200247.
Hutchin TP, Parker MJ, Young ID, Davis AC, Pulleyn LJ, Deeble J, Lench NJ, Markham AF, Mueller RF: A novel mutation in the mitochondrial tRNA(Ser(UCN)) gene in a family with non-syndromic sensorineural hearing impairment. J Med Genet. 2000, 37: 692-694. 10.1136/jmg.37.9.692.
Rydzanicz M, Wrobel M, Pollak A, Gawecki W, Brauze D, Kostrzewska-Poczekaj M, Wojsyk-Banaszak I, Lechowicz U, Mueller-Malesinska M, Oldak M, et al: Mutation analysis of mitochondrial 12S rRNA gene in Polish patients with non-syndromic and aminoglycoside-induced hearing loss. Biochem Biophys Res Commun. 2010, 395: 116-121. 10.1016/j.bbrc.2010.03.149.
Chaig MR, Zernotti ME, Soria NW, Romero OF, Romero MF, Gerez NM: A mutation in mitochondrial 12S rRNA, A827G, in Argentinean family with hearing loss after aminoglycoside treatment. Biochem Biophys Res Commun. 2008, 368: 631-636. 10.1016/j.bbrc.2008.01.143.
Bacino C, Prezant TR, Bu X, Fournier P, Fischel-Ghodsian N: Susceptibility mutations in the mitochondrial small ribosomal RNA gene in aminoglycoside induced deafness. Pharmacogenetics. 1995, 5: 165-172. 10.1097/00008571-199506000-00005.
Li Z, Li R, Chen J, Liao Z, Zhu Y, Qian Y, Xiong S, Heman-Ackah S, Wu J, Choo DI, et al: Mutational analysis of the mitochondrial 12S rRNA gene in Chinese pediatric subjects with aminoglycoside-induced and non-syndromic hearing loss. Hum Genet. 2005, 117: 9-15. 10.1007/s00439-005-1276-1.
Thyagarajan D, Bressman S, Bruno C, Przedborski S, Shanske S, Lynch T, Fahn S, DiMauro S: A novel mitochondrial 12SrRNA point mutation in parkinsonism, deafness, and neuropathy. Ann Neurol. 2000, 48: 730-736. 10.1002/1531-8249(200011)48:5<730::AID-ANA6>3.0.CO;2-0.
Tessa A, Giannotti A, Tieri L, Vilarinho L, Marotta G, Santorelli FM: Maternally inherited deafness associated with a T1095C mutation in the mDNA. Eur J Hum Genet. 2001, 9: 147-149. 10.1038/sj.ejhg.5200601.
Wang Q, Li R, Zhao H, Peters JL, Liu Q, Yang L, Han D, Greinwald JH, Young WY, Guan MX: Clinical and molecular characterization of a Chinese patient with auditory neuropathy associated with mitochondrial 12S rRNA T1095C mutation. Am J Med Genet A. 2005, 133A: 27-30. 10.1002/ajmg.a.30424.
Zhao L, Young WY, Li R, Wang Q, Qian Y, Guan MX: Clinical evaluation and sequence analysis of the complete mitochondrial genome of three Chinese patients with hearing impairment associated with the 12S rRNA T1095C mutation. Biochem Biophys Res Commun. 2004, 325: 1503-1508. 10.1016/j.bbrc.2004.10.199.
Lu J, Li Z, Zhu Y, Yang A, Li R, Zheng J, Cai Q, Peng G, Zheng W, Tang X, et al: Mitochondrial 12S rRNA variants in 1642 Han Chinese pediatric subjects with aminoglycoside-induced and nonsyndromic hearing loss. Mitochondrion. 2010, 10: 380-390. 10.1016/j.mito.2010.01.007.
Konings A, Van Camp G, Goethals A, Van Eyken E, Vandevelde A, Ben Azza J, Peeters N, Wuyts W, Smeets H, Van Laer L: Mutation analysis of mitochondrial DNA 12SrRNA and tRNASer(UCN) genes in non-syndromic hearing loss patients. Mitochondrion. 2008, 8: 377-382. 10.1016/j.mito.2008.08.001.
Kobayashi K, Oguchi T, Asamura K, Miyagawa M, Horai S, Abe S, Usami S: Genetic features, clinical phenotypes, and prevalence of sensorineural hearing loss associated with the 961delT mitochondrial mutation. Auris Nasus Larynx. 2005, 32: 119-124. 10.1016/j.anl.2005.01.010.
Yao YG, Salas A, Bravi CM, Bandelt HJ: A reappraisal of complete mtDNA variation in East Asian families with hearing impairment. Hum Genet. 2006, 119: 505-515. 10.1007/s00439-006-0154-9.
Herrnstadt C, Howell N: An evolutionary perspective on pathogenic mtDNA mutations: haplogroup associations of clinical disorders. Mitochondrion. 2004, 4: 791-798. 10.1016/j.mito.2004.07.041.
Kong QP, Bandelt HJ, Sun C, Yao YG, Salas A, Achilli A, Wang CY, Zhong L, Zhu CL, Wu SF, et al: Updating the East Asian mtDNA phylogeny: a prerequisite for the identification of pathogenic mutations. Hum Mol Genet. 2006, 15: 2076-2086. 10.1093/hmg/ddl130.
Tanaka M, Cabrera VM, Gonzalez AM, Larruga JM, Takeyasu T, Fuku N, Guo LJ, Hirose R, Fujita Y, Kurata M, et al: Mitochondrial genome variation in eastern Asia and the peopling of Japan. Genome Res. 2004, 14: 1832-1850. 10.1101/gr.2286304.
Achilli A, Perego UA, Bravi CM, Coble MD, Kong QP, Woodward SR, Salas A, Torroni A, Bandelt HJ: The phylogeny of the four pan-American MtDNA haplogroups: implications for evolutionary and disease studies. PLoS One. 2008, 3: e1764-10.1371/journal.pone.0001764.
Usami S, Abe S, Akita J, Namba A, Shinkawa H, Ishii M, Iwasaki S, Hoshino T, Ito J, Doi K, et al: Prevalence of mitochondrial gene mutations among hearing impaired patients. J Med Genet. 2000, 37: 38-40. 10.1136/jmg.37.1.38.
Oshima T, Kudo T, Ikeda K: Point mutation of the mitochondrial genome in Japanese deaf-mutism. ORL J Otorhinolaryngol Relat Spec. 2001, 63: 329-332. 10.1159/000055769.
He Y, Wu J, Dressman DC, Iacobuzio-Donahue C, Markowitz SD, Velculescu VE, Diaz LA, Kinzler KW, Vogelstein B, Papadopoulos N: Heteroplasmic mitochondrial DNA mutations in normal and tumour cells. Nature. 2010, 464: 610-614. 10.1038/nature08802.
Lu J, Wang D, Li R, Li W, Ji J, Zhao J, Ye W, Yang L, Qian Y, Zhu Y, et al: Maternally transmitted diabetes mellitus associated with the mitochondrial tRNA(Leu(UUR)) A3243G mutation in a four-generation Han Chinese family. Biochem Biophys Res Commun. 2006, 348: 115-119. 10.1016/j.bbrc.2006.07.010.
Mancuso M, Filosto M, Forli F, Rocchi A, Berrettini S, Siciliano G, Murri L: A non-syndromic hearing loss caused by very low levels of the mtDNA A3243G mutation. Acta Neurol Scand. 2004, 110: 72-74. 10.1111/j.1600-0404.2004.00254.x.
Laloi-Michelin M, Meas T, Ambonville C, Bellanne-Chantelot C, Beaufils S, Massin P, Vialettes B, Gin H, Timsit J, Bauduceau B, et al: The clinical variability of maternally inherited diabetes and deafness is associated with the degree of heteroplasmy in blood leukocytes. J Clin Endocrinol Metab. 2009, 94: 3025-3030. 10.1210/jc.2008-2680.
van Den Bosch BJ, de Coo RF, Scholte HR, Nijland JG, van Den Bogaard R, de Visser M, de Die-Smulders CE, Smeets HJ: Mutation analysis of the entire mitochondrial genome using denaturing high performance liquid chromatography. Nucleic Acids Res. 2000, 28: E89-10.1093/nar/28.20.e89.
Matsunaga T, Hirota E, Bito S, Niimi S, Usami S: Clinical course of hearing and language development in GJB2 and non-GJB2 deafness following habilitation with hearing aids. Audiol Neurootol. 2006, 11: 59-68. 10.1159/000089607.
Matsunaga T, Kumanomido H, Shiroma M, Ohtsuka A, Asamura K, Usami S: Deafness due to A1555G mitochondrial mutation without use of aminoglycoside. Laryngoscope. 2004, 114: 1085-1091. 10.1097/00005537-200406000-00024.
Wada Y, Kayamori Y, Hamasaki N: Protocols for entire mitochondrial DNA sequence by direct sequencing. Rinsho-Kensa. 2005, 49: 59-64. (Japanese)
Hereditary Hearing Loss Homepage. [http://hereditaryhearingloss.org/]
Anderson S, Bankier AT, Barrell BG, de Bruijn MH, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, et al: Sequence and organization of the human mitochondrial genome. Nature. 1981, 290: 457-465. 10.1038/290457a0.
Andrews RM, Kubacka I, Chinnery PF, Lightowlers RN, Turnbull DM, Howell N: Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA. Nat Genet. 1999, 23: 147-10.1038/13779.
mtSNP database. [http://mtsnp.tmig.or.jp/mtsnp/index.shtml]
Uppsala mtDB database. [http://www.genpat.uu.se/mtDB/]
Leveque M, Marlin S, Jonard L, Procaccio V, Reynier P, Amati-Bonneau P, Baulande S, Pierron D, Lacombe D, Duriez F, et al: Whole mitochondrial genome screening in maternally inherited non-syndromic hearing impairment using a microarray resequencing mitochondrial DNA chip. Eur J Hum Genet. 2007, 15: 1145-1155. 10.1038/sj.ejhg.5201891.
Hamada M, Kiryu H, Sato K, Mituyama T, Asai K: Prediction of RNA secondary structure using generalized centroid estimators. Bioinformatics. 2009, 25: 465-473. 10.1093/bioinformatics/btn601.
Sato K, Hamada M, Asai K, Mituyama T: CENTROIDFOLD: a web server for RNA secondary structure prediction. Nucleic Acids Res. 2009, 37: W277-280. 10.1093/nar/gkp367.
Mollers M, Maniura-Weber K, Kiseljakovic E, Bust M, Hayrapetyan A, Jaksch M, Helm M, Wiesner RJ, von Kleist-Retzow JC: A new mechanism for mtDNA pathogenesis: impairment of post-transcriptional maturation leads to severe depletion of mitochondrial tRNASer(UCN) caused by T7512C and G7497A point mutations. Nucleic Acids Res. 2005, 33: 5647-5658. 10.1093/nar/gki876.
Tang X, Yang L, Zhu Y, Liao Z, Wang J, Qian Y, Tao Z, Hu L, Wu G, Lan J, et al: Very low penetrance of hearing loss in seven Han Chinese pedigrees carrying the deafness-associated 12S rRNA A1555G mutation. Gene. 2007, 393: 11-19. 10.1016/j.gene.2007.01.001.
Torroni A, Campos Y, Rengo C, Sellitto D, Achilli A, Magri C, Semino O, Garcia A, Jara P, Arenas J, et al: Mitochondrial DNA haplogroups do not play a role in the variable phenotypic presentation of the A3243G mutation. Am J Hum Genet. 2003, 72: 1005-1012. 10.1086/373936.
Takahashi K, Merchant SN, Miyazawa T, Yamaguchi T, McKenna MJ, Kouda H, Iino Y, Someya T, Tamagawa Y, Takiyama Y, et al: Temporal bone histopathological and quantitative analysis of mitochondrial DNA in MELAS. Laryngoscope. 2003, 113: 1362-1368. 10.1097/00005537-200308000-00018.
Tiranti V, Chariot P, Carella F, Toscano A, Soliveri P, Girlanda P, Carrara F, Fratta GM, Reid FM, Mariotti C, et al: Maternally inherited hearing loss, ataxia and myoclonus associated with a novel point mutation in mitochondrial tRNASer(UCN) gene. Hum Mol Genet. 1995, 4: 1421-1427. 10.1093/hmg/4.8.1421.
Tang X, Li R, Zheng J, Cai Q, Zhang T, Gong S, Zheng W, He X, Zhu Y, Xue L, et al: Maternally inherited hearing loss is associated with the novel mitochondrial tRNA Ser(UCN) 7505T > C mutation in a Han Chinese family. Mol Genet Metab. 2010, 100: 57-64. 10.1016/j.ymgme.2010.01.008.
Sue CM, Tanji K, Hadjigeorgiou G, Andreu AL, Nishino I, Krishna S, Bruno C, Hirano M, Shanske S, Bonilla E, et al: Maternally inherited hearing loss in a large kindred with a novel T7511C mutation in the mitochondrial DNA tRNA(Ser(UCN)) gene. Neurology. 1999, 52: 1905-1908.
Young WY, Zhao L, Qian Y, Wang Q, Li N, Greinwald JH, Guan MX: Extremely low penetrance of hearing loss in four Chinese families with the mitochondrial 12S rRNA A1555G mutation. Biochem Biophys Res Commun. 2005, 328: 1244-1251. 10.1016/j.bbrc.2005.01.085.
Rydzanicz M, Wrobel M, Cywinska K, Froehlich D, Gawecki W, Szyfter W, Szyfter K: Screening of the general Polish population for deafness-associated mutations in mitochondrial 12S rRNA and tRNA Ser(UCN) genes. Genet Test Mol Biomarkers. 2009, 13: 167-172. 10.1089/gtmb.2008.0098.
The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2350/12/135/prepub
Acknowledgements and Funding
This study was supported by a Health Science Research Grant (H16-kankakuki-006 to TM) from the Ministry of Health, Labor, and Welfare of Japan, a Grant-in-Aid for Clinical Research to TM from the National Hospital Organization, and by a Grant-in-Aid for Scientific Research (19592001 to TM) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
The authors declare that they have no competing interests.
HM participated in cloning and sequencing, data analysis, and drafted the manuscript. HKo, ET, ITK, and HKa established and conducted the dHPLC analysis, sequencing, and data analysis. HT, SU, and TO coordinated the study and helped with gene analysis. TM planned and organized the study, examined patients, analyzed data, and helped draft the manuscript. All the authors read and approved the final manuscript.
Electronic supplementary material
About this article
Cite this article
Mutai, H., Kouike, H., Teruya, E. et al. Systematic analysis of mitochondrial genes associated with hearing loss in the Japanese population: dHPLC reveals a new candidate mutation. BMC Med Genet 12, 135 (2011). https://doi.org/10.1186/1471-2350-12-135
- Hearing Loss
- Sensorineural Hearing Loss
- Normal Hearing
- Severe Hearing Loss
- Candidate Mutation