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Systematic analysis of mitochondrial genes associated with hearing loss in the Japanese population: dHPLC reveals a new candidate mutation
© Mutai et al; licensee BioMed Central Ltd. 2011
Received: 6 June 2011
Accepted: 12 October 2011
Published: 12 October 2011
Variants of mitochondrial DNA (mtDNA) have been evaluated for their association with hearing loss. Although ethnic background affects the spectrum of mtDNA variants, systematic mutational analysis of mtDNA in Japanese patients with hearing loss has not been reported.
Using denaturing high-performance liquid chromatography combined with direct sequencing and cloning-sequencing, Japanese patients with prelingual (N = 54) or postlingual (N = 80) sensorineural hearing loss not having pathogenic mutations of m.1555A > G and m.3243A > G nor GJB2 were subjected to mutational analysis of mtDNA genes (12S rRNA, tRNA Leu(UUR) , tRNA Ser(UCN) , tRNA Lys , tRNA His , tRNA Ser(AGY) , and tRNA Glu ).
We discovered 15 variants in 12S rRNA and one homoplasmic m.7501A > G variant in tRNA Ser(UCN) ; no variants were detected in the other genes. Two criteria, namely the low frequency in the controls and the high conservation among animals, selected the m.904C > T and the m.1105T > C variants in 12S rRNA as candidate pathogenic mutations. Alterations in the secondary structures of the two variant transcripts as well as that of m.7501A > G in tRNA Ser(UCN) were predicted.
The m.904C > T variant was found to be a new candidate mutation associated with hearing loss. The m.1105T > C variant is unlikely to be pathogenic. The pathogenicity of the homoplasmic m.7501T > A variant awaits further study.
Hearing loss manifests in more than 1 in 1000 persons at birth, and the frequency increases subsequently to 3 in 1000 by 4 years of age [1, 2]. Approximately 50 to 70% of congenital and childhood deafness is estimated to be due to genetic mutations. In adults, the prevalence of hereditary hearing impairment has been estimated to be approximately 3.2 in 1000 . Some of the mitochondrial DNA (mtDNA) genes, such as 12S rRNA, tRNA Leu(UUR) , and tRNA Ser(UCN) , are known to be responsible for hereditary hearing loss . Among them, the m.1555A > G mutation in 12S rRNA is found relatively frequently (0.6-16%, depending on the ethnic group) in aminoglycoside-induced, congenital, and late-onset nonsyndromic hearing loss [4, 5]. The m.1494C > T mutation in 12S rRNA is also associated with aminoglycoside-induced and nonsyndromic hearing loss [6, 7]. The m.3243A > G mutation in tRNA Leu(UUR) is associated with late-onset nonsyndromic hearing loss [8, 9], maternally inherited diabetes and deafness (MIDD) [10, 11], and mitochondrial myopathy, encephalopathy, lactic acidosis, stroke-like episodes (MELAS), which frequently presents with hearing loss [12, 13]. The m.7445A > C/G/T [14–16], 7472insC , and 7510T > C mutations  in tRNA Ser(UCN) are also associated with aminoglycoside-induced, nonsyndromic, or syndromic hearing loss.
In addition, many other variants in 12S rRNA have been proposed to be associated with hearing loss . Some variants such as m.827A > G [19, 20], 961T > C , 961delT + Cn [21, 22], 1005T > C , and 1095T > C in 12S rRNA [22–26] are not definitively related to hearing loss, because they have been found in subjects with normal hearing and/or are not conserved among mammals [19, 27–30]. Moreover, a variety of mitochondrial haplogroups often localize in specific ethnic groups, making it difficult to determine whether the mtDNA variants are associated directly with diseases, indirectly as risk factors, or simply with rare subhaplogroups [31–34]. Accumulating reports of various novel mtDNA mutations associated with hearing loss prompted us to evaluate these variants in patients with hearing loss in Japan, where mtDNA mutation studies have focused on a few limited nucleotide positions [35, 36].
A single cell contains hundreds of mitochondria, and the mtDNA in each mitochondrion is occasionally heterogeneous, a feature called heteroplasmy . The proportion of pathogenic mutations of heteroplasmic mtDNA is considered to be one of the reasons for the wide range of severity of phenotypes seen in patients with mitochondrial-related diseases, such as those reported in the case of the m.3243A > G mutation [38–40]. Denaturing high-performance liquid chromatography (dHPLC) is a sensitive method to detect heteroplasmic mutations that can be overlooked by simple direct sequencing and comparison of the scanned peaks or restriction fragment length polymorphism-PCR [28, 41]. In this study, we conducted a systematic mutational analysis of mtDNA by dHPLC combined with direct sequencing and cloning-sequencing in samples from Japanese patients with hearing loss.
Subjects with bilateral sensorineural hearing loss were recruited by the National Tokyo Medical Center and collaborating hospitals. Subjects' medical histories were obtained and physical examinations were performed to exclude those subjects with syndromic symptoms, diseases of the outer or/and middle ear, and environmental factors related to hearing loss such as history of infectious diseases, premature birth, and newborn meningitis. Patients with a history of use of ototoxic drugs were included in the study because these drugs are known to be associated with mitochondrial hearing loss. Prior to this study, the patients were confirmed not to have the m.1555A > G and m.3243A > G mutations or not to be diagnosed as having GJB2 -caused hearing loss, as assessed by restriction fragment length polymorphism-PCR or together with direct sequencing if the heterozygotic 235delC mutation was detected in GJB2 [42, 43]. The 134 subjects were classified into prelingual hearing loss (onset before 5 years old, 20 males and 34 females) or postlingual hearing loss (onset at 5 years old or later, 31 males and 49 females) . The control group consisted of 137 unrelated Japanese individuals with normal hearing as examined by pure-tone audiometry. All subjects or their parents gave prior informed consent for participation in this study. This study was approved by the ethics committee of National Tokyo Medical Center.
Screening for mtDNA mutations by dHPLC
DNA was extracted from blood samples using the Gentra Puregene DNA isolation kit (QIAGEN, Hamburg, Germany). Initially, whole mtDNA from each patient was amplified in three overlapping fragments (1351-8197, 6058-12770, and 11706-2258)  by LATaq DNA polymerase (TaKaRa BIO, Shiga, Japan). PCR was conducted at 94°C for 1 min followed by 30 cycles of 98°C for 10 s and 68°C for 6.5 min. Then, using the PCR products as templates, variants were analyzed by the Mitoscreen assay kit (Transgenomic, Glasgow, UK). We amplified the genes 12S rRNA, tRNA Leu(UUR) , tRNA Ser(UCN) , tRNA Lys , tRNA His , tRNA Ser(AGY) , and tRNA Glu , for which mutations were reported to be associated with hearing loss on the Hereditary Hearing Loss Homepage  when the study was started. The PCR products using primer sets MT4 (for 12S rRNA), MT6 (tRNA Leu(UUR) ), MT10 (tRNA Ser(UCN) ), MT11 (tRNA Lys ), MT15 (tRNA His and tRNA Ser(AGY) ), and MT18 (tRNA Glu ) were incubated with the appropriate restriction enzymes, incubated for heteroduplex formation either with reference PCR products to detect homoplasmy or with their own PCR products to detect heteroplasmy, then analyzed by dHPLC (WAVE system, Transgenomic) according to the manufacturer's protocols.
The reference mtDNA was derived from a Japanese individual with normal hearing. Sequencing of the entire reference mtDNA revealed 750A > G and 1438A > G polymorphisms, and the mtDNA sequence was otherwise comparable to the revised Cambridge Reference sequence (AC_000021) [46, 47]).
When homoplasmic or heteroplasmic variants were detected, the PCR product was subjected to direct sequencing by the BigDye Terminator ver. 3 cycle sequencing kit and ABI genetic analyzer 3730 (Life Technologies, Carlsbad, CA). To sequence 12S rRNA, an additional nested PCR product (656-1,266) was amplified with primers F (5'-tggtcctagcctttctattagctctt-3') and R (5'-tggcggtatataggctgagca-3'). To sequence tRNA Ser(UCN) , an additional nested PCR product (7,209-7,609) was amplified with primers F (5'-atgccccgacgttactcg-3') and R (5'- acctacttgcgctgcatgtg-3'). To determine the proportion of heteroplasmic 1005T > C variant in the 12S rRNA, the nested PCR (656-1,266) product was cloned and sequenced. Nested PCR was carried out by replacing AmpliTaq Gold DNA polymerase with PrimeSTAR DNA polymerase, which has 3'-proofreading activity (TaKaRa BIO), followed by the Zero Blunt TOPO PCR cloning kit (Life Technologies). We sequenced 54 clones derived from the proband mtDNA and 24 clones derived from the mtDNA of each of five siblings. Sequencing data were analyzed by SeqScape ver2.6 (Life Technologies) and DNASIS Pro (Hitachisoft, Tokyo, Japan). The sequencing results for each patient were compared with the revised Cambridge Reference sequence to identify mtDNA variants. The uniqueness of each mutation was evaluated by comparison with the mtSNP database , MITOMAP , and the Uppsala mtDB database .
Prediction of pathogenicity of mtDNA variants
The variants were evaluated based on double selection as proposed by Leveque and coworkers , with modification. Initially, we measured the frequencies of each variant found in the controls in our study (N = 137) and in the mtSNP database (N = 672, including: centenarians in Gifu, centenarians in Tokyo, type 2 diabetes mellitus patients (without or with vascular disorders), overweight young adult males, non-overweight young adult males, Parkinson's disease patients, and Alzheimer's disease patients in Japan). The variants with a frequency of more than 3% in one of the groups were considered as non-pathologic polymorphisms. We used a frequency threshold lower than that previously used (4%)  because the mtSNP database of Japanese individuals and the controls reflect the patient ethnic group background more closely than the mtDB and therefore requires a lower frequency threshold to exclude polymorphisms. The nucleotide conservation in each gene from human and 50 mammalian species was evaluated by ClustalW. The additional file lists the mammalian species and the accession numbers of the mtDNA (Additional File 1: Table S1). The variant frequencies in the mtDB were calculated to determine if the low variant frequencies measured in the controls reflect rare haplotypes in the Japanese population and are more common worldwide. All the variants were also analyzed with PhyloTree (mtDNA tree Build 10)  to search for previously characterized variants in haplogroups. Pathogenicity of the variants was also evaluated by predicting the secondary structures of the mitochondrial transcripts with or without the variant using Centroid Fold [53, 54].
Mitochondrial DNA variants identified in this study
(N = 54)
(N = 80)
(N = 137)
freq in controls (%)
(N = 672)a
freq in Japanese (%)
(N = 2704)
freq in mtDB (%)
663A > G
709G > A
750A > G
752C > T
827A > G
904C > T
1005T > C
1009C > T
1041A > G
1107T > C
1119T > C
1382A > C
1438A > G
7501T > A
In our study, screening of mtDNA by dHPLC and direct sequencing detected 15 variants in 12S rRNA and 1 variant in tRNA Ser(UCN) . Comparison of the variant frequencies in controls, assessment of nucleotide conservation among mammalian species, and structural analysis of the transcript was used to select candidate mutations associated with hearing loss. No variants in tRNA Leu(UUR) , tRNA Lys , tRNA His , tRNA Ser(AGY) , or tRNA Glu were detected in the subjects studied here, suggesting that the mutations in these genes associated with hearing loss are not common in the Japanese population.
To our knowledge, the homoplasmic m.904C > T variant in 12S rRNA has not been reported elsewhere. Lack of symptoms in the maternal relatives does not exclude mitochondrial transmission, because penetrance of 12S rRNA mutations can be extremely low, as seen in the m.1555A > G associated with hearing loss . Conservation of the nucleotides among mammals and gross alteration of the predicted secondary structure of the 12S rRNA transcript suggest that the m.904C > T variant might affect auditory function by changing the efficiency with which mRNAs are transcribed to yield mitochondrial proteins.
A patient with the homoplasmic m.1005T > C variant in the 12S rRNA had a child with prelingual hearing loss. The inheritance of hearing loss in the child is likely due to the transmission of an autosomal mutation, not mtDNA, from the male proband. Therefore, the data for this family may not provide unequivocal information about the pathogenicity of the m.1005T > C variant [4, 22, 27, 30].
Identification of the heteroplasmic m.1005T > C variant in a patient with hearing loss is a novel finding, because this variant has been known only as homoplasmic [22, 27, 30, 34]. We did not verify that the heteroplasmic m.1005T > C variant was correlated with hearing loss because four of five siblings of the proband had hearing loss without carrying the variant, whereas it might be associated with diabetes mellitus. However, it is difficult to exclude the possibility of association of the heteroplasmic variant detected in blood samples with mitochondrial diseases such as deafness. Frequencies of heteroplasmy of mtDNA vary considerably among tissues in the same individual (for instance, [37, 57, 58]). Therefore, it is possible that the frequency of the m.1005T > C variant in the inner ear cells of the siblings is much higher than in the blood cells and thus may underlie the hearing loss.
Another finding in this study is that three patients with postlingual hearing loss had the homoplasmic m.7501T > A variant in tRNA Ser (UCN) . Various mutations in tRNA Ser(UCN) , such as m.7445A > G [15, 16], 7472insC [17, 59], 7505T > C , 7510T > C , and 7511T > C [51, 59, 61], are associated with various types of hearing loss (syndromic or nonsyndromic, prelingual or late-onset), raising the possibility that the m.7501T > A variant, reported elsewhere without detailed investigation , is also associated with hearing loss. The low conservation of the variation at this position (29% among mammals) does not support the pathogenicity of the variant, in contrast to the much higher conservation at m.7472A (61%), 7505A (98%), 7510T (78%), and 7511T (98%). On the other hand, the m.7501T > A variant is predicted to modify the secondary structure of the D-arm in the tRNA Ser(UCN) transcript; the D-arm is important for the stability of the transcript and the general rate of mitochondrial protein synthesis . Further investigation, such as haplogroup analysis or generating lymphoblastoid cell lines to measure endogenous respiration rates, may help to define the pathogenicity of the m.7501T > A variant.
All other variants found in this study, such as m.827A > G, 961insC, and 961delT + Cn, which have been discussed elsewhere with respect to their pathogenicity [21, 22, 27, 30, 62], were considered to be non-pathologic polymorphisms because they were found frequently in the controls. The other variants, m.663A > G, 709G > A, 750A > G, 752C > T, 1009C > T, 1041A > G, 1107T > C, 1119T > C, 1382A > C, and 1438A > G, were frequently detected in the controls and considered to be nonpathogenic polymorphisms, which is in consistent with a previous report . The spectrum of variants of mitochondrial genes in Japanese individuals was similar to that in a Chinese population , for which most of the variants detected in this study (other than the m.904C > T and 7501T > A) have been reported. In contrast, the spectrum was dissimilar to those in other ethnic groups such as the Polish population [19, 63]. Our results indicate that ethnic background should be taken into consideration when studying the pathogenicity of mtDNA variants based on their frequencies in controls.
We sought to detect mitochondrial variants other than m.1555A > G or 3243A > G mutations, which are known to be related to hearing loss, by dHPLC, direct sequencing, and cloning-sequencing in samples from Japanese patients with hearing loss. The homoplasmic m.904C > T variant in 12S rRNA was considered to be a new candidate mutation associated with hearing loss. The pathogenicity of the m.7501T > A variant in tRNA Ser(UCN) remains inconclusive, and other variants identified in this study, including the heteroplasmic m.1005T > C variant, are not positively associated with hearing loss. No variants were detected in the in tRNA Leu(UUR) , tRNA Lys , tRNA His , tRNA Ser(AGY) , and tRNA Glu .
Acknowledgements and Funding
This study was supported by a Health Science Research Grant (H16-kankakuki-006 to TM) from the Ministry of Health, Labor, and Welfare of Japan, a Grant-in-Aid for Clinical Research to TM from the National Hospital Organization, and by a Grant-in-Aid for Scientific Research (19592001 to TM) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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