- Research article
- Open Access
- Open Peer Review
The neck-region polymorphism of DC-SIGNR in peri-centenarian from Han Chinese Population
- Hui Li†1, 2, 3, 4,
- Cheng-Ye Wang†1, 2, 3, 4,
- Jia-Xin Wang†2,
- Nelson Leung-Sang Tang4, 5, 6,
- Liang Xie1, 2, 3, 4,
- Yuan-Ying Gong1, 4,
- Zhao Yang7,
- Liang-You Xu8,
- Qing-Peng Kong1, 4 and
- Ya-Ping Zhang1, 2, 4Email author
© Li et al; licensee BioMed Central Ltd. 2009
- Received: 31 March 2009
- Accepted: 14 December 2009
- Published: 14 December 2009
DC-SIGNR (also called CD209L) has been extensively studied on its role in host genetic predisposition to viral infection. In particular, variable number tandem repeat (VNTR) of the neck-region of DC-SIGNR is highly polymorphic and the polymorphism has been investigated for genetic predisposition to various infectious diseases, though conflicting results had been reported. As infection is a major cause of human death and a mechanism of natural selection, we hypothesized that VNTR polymorphism of DC-SIGNR might have an effect on human life span.
Here we collected 361 peri-centenarian individuals (age ≥94 for female and age ≥90 for male) and 342 geographically matched controls (age 22-53, mean 35.0 ± 12.0) from Han Chinese. The VNTR polymorphism of the neck region was determined by PCR and genotype was called by separating the PCR products in agarose gel.
A total of 11 genotypes and 5 alleles were found in our population. The genotype distribution, allele frequencies and homozygote proportion did not show a significant difference between peri-centenarian and control group. As gender differences in lifespan are ubiquitously observed throughout the animal kingdom, we then stratified the samples by gender. There was more 6/7 genotypes in female peri-centenarian group than that in female control group, at a marginal level of significance (5.56 vs. 1.28%, p = 0.041). The difference was not significant after correction by Bonferroni method. It suggests a possible differential effect of DC-SIGNR VNTR genotypes between sexes. Further studies are warranted to confirm our preliminary findings and investigate the mechanisms of the underlying functions.
Our study indicated that there was absence of association between the neck region polymorphism of DC-SIGNR and longevity in Han Chinese population. But the question of whether the DC-SIGNR could affect longevity in a gender-specific pattern remains open.
- Neck Region
- Dengue Virus
- Variable Number Tandem Repeat
- Carbohydrate Recognition Domain
- Marginal Level
Dendritic cell-specific intracellular adhesion molecular-3-grabbing nonintegrin (DC-SIGN) and DC-SIGN related (DC-SIGNR) are C-type lectins involved in both innate and adaptive immunity. As DC-SIGN and DC-SIGNR originated from the duplication of the same ancestral gene, they are located side-by-side within a ~26-kb segment of chromosome 19p13.2-3 and they share similar functions as cell adhesion receptors and pathogen recognition receptors. A large number of their binding targets are of clinical significance, including bacteria such as Mycobacterium tuberculosis, Helicobacter pylori, and certain Klebsiela pneumonia strains, as well as virus, such as HIV-1, HIV-2, Ebola virus, cytomegalovirus, hepatitis C virus(HCV), Dengue virus, and SARS-coronavirus [2–5]. One difference between DC-SIGN and DC-SIGNR is that DC-SIGN is expressed primarily on phagocytic cells, such as dendritic cells and macrophages, whereas DC-SIGNR expression is restricted to endothelial cells in liver, lymph nodes and placental capillaries[6, 7].
DC-SIGNR is a type II transmembrane protein composed of three domains: an N-terminal cytoplasmic domain, a neck region made up of a highly conserved 23-amino-acid repeat, and a C-terminal extracellular C-type carbohydrate recognition domain (CRD) involved in pathogen binding. The neck region connects the CRD from the transmembrane region and assembles the lectin into a tetrameric protein conformation on the cell surface. Thus the neck region is responsible for the homo-oligomerization that brings the CRDs into proximity for high-affinity ligand binding. On the other hand, heterozygous expression of DC-SIGNR of different length in the neck region may lead to reduced ligand-binding affinity and loss of the efficient recognition of multivalent ligands[8, 10]. Furthermore, the length variations of this neck region can critically influence the pathogen-binding properties of the CRD of these proteins, as some studies have found associations between length variation of the neck region and susceptibility to various infectious diseases, such as HIV, HCV, and SARS [11–17], though conflicting results were also found. These infectious diseases affect an individual's life span, since they can be life-threatening and may lead to early death if lacking effective treatments. This is one of the mechanisms for natural selection in a population.
It has been suggested that polymorphisms in immune-related genes not only affect susceptibility to infectious diseases, but also influence the aging process through immunosenescence. Because the highly polymorphic DC-SIGNR neck region plays such a vital role in host genetic predisposition to a number of infectious diseases, we hypothesized that it would also influence the aging process and might be a genetic factor for longevity. To confirm this hypothesis, we collected 361 unrelated peri-centenarian individuals (age ≥94 for female and age ≥90 for male) and 342 normal controls (age 22-53, mean 35.0 ± 12.0) in Han Chinese population, and genotyped their VNTR polymorphism of DC-SIGNR neck region. To the best of our knowledge, this is the first study on the association between VNTR polymorphism in DC-SIGNR gene and longevity.
This elderly Han Chinese cohort composed of 485 unrelated peri-centenarian individuals (age ≥94 for female and age ≥90 for male; mean age 95.14 ± 2.40) were recruited from Dujiangyan in Sichuan province of China. As reported in previous publication, ages of the subjects were authenticated by the official certification of the Fifth Nation Census in China which was also supported by information of the number of offspring generations (≥3), and local village records. Only subjects with their age supported by both government identity record, local village record and fulfilled the required number of offspring generation were included.
An interview was carried out for each peri-centenarian subjects at their homes by physicians during a home visit session. A brief medical history and examination was carried out to exclude participants with clinically apparent diseases (such as chronic lung disease, chronic heart failure, severe vision disorders, severe hearing disorders, hypertension, fracture, and arthritis severly limiting activity of daily living). More than 500 peri-centenarian individuals (age ≥94 for female and age ≥90 for male) have been interviewed but only 485 individuals (246 males and 239 females) who fulfilled all inclusion criteria, were included into this peri-centenarian cohort.
As we have more samples to fit into four 96-well plates for genotyping (each plate holds 90 samples while the reminding wells were used for QC samples and negative control, i.e. 360 were genotyped in these four 96-well plates), we blindly pull out a block of 180 male and 180 female samples for genotyping. One additional male sample was used for reaction optimization and his genotype results was also included, which led to a total of 181 male genotyping results.
Meanwhile, 342 healthy younger individuals, which included 156 females and 186 males, were also recruited from the same area, to serve as a control group (aged 22-53, mean age 35.0 ± 12.0). The demographic characteristics of these samples have been described in our previous work. All participants signed informed consent after they had been given a clear explanation of the potential risk of the study according to the Declaration of Helsinki. This research has been approved by the Ethics Committee on human experimentation of Kunming Institute of Zoology, Chinese Academy of Sciences and institutions in Dujiangyan.
Genotyping and Statistical Methods
Peripheral blood was collected from the participants and genomic DNA was extracted using the standard phenol/chloroform method. The VNTR polymorphism of the neck region was genotyped using the assay described in Chan et al. . The genotype was determined by separating the PCR products in 3% agarose gel with ethidium bromide. To validate the genotyping results, 10% of the samples were re-genotyped by either duplicated genotyping experiments or direct sequencing.
Hardy-Weinberg equilibrium (HWE) test for VNTR in the peri-centenarian and control groups was performed by GENEPOP software http://wbiomed.curtin.edu.au/genepop/index.html. Homozygote proportions, genotype distribution and allele frequencies were compared by two-tailed chi-square test or Fisher's exact test (SPSS 11.5, SPSS Inc) (df = number of genotypes being analysed minus 1). When P value < 0.05, it was considered to be statistically significant and further subjected to Bonferroni correction for multiple testing.
Sample size determination
Allele and genotype distribution of encoding VNTR in DC-SIGNR gene in Chinese populations of different parts of China have been surveyed and the data have been published in our previous work. Based on the frequencies of various alleles of the VNTR (Additional File 1: supplementary table S1), the sample size (number of chromosomes) required to show an allelic odd ratio of 1.5 and 2 were determined by the program STATCALC (CDC, USA). For example, the 5-repeat allele was found at an allelic frequency of 0.16 in Han population. A sample of 657 cases and 657 controls chromosomes is required to show an effect caused by an allelic odd ratios of 1.5 at power of 80% at a 2-sided α value of 0.05. Details of sample size required in various scenario of association allele and odd ratios are shown in Additional file 1: supplementary table S2. It was found that, for most of the prevalent VNTR alleles, our sample has sufficient power to detect an association with allelic odd ratios of 2, except for the allele of 8-repeats, which was found at a MAF of 0.002.
Genotype, allele distribution and homozygote proportion of DC-SIGNR neck region in peri-centenarian and control group.
(N = 361)
(N = 342)
Genotype, allele distribution and homozygote proportion of DC-SIGNR neck region in peri-centenarian and control group stratified by gender.
N = 180
N = 156
N = 181
N = 186
Genetic factors are crucial in the ageing process and thus, also influence longevity. Mitchell et al. estimated a 25% heritability for the life-span in an Amish population in Lancaster County, Pennsylvania, which implied a relatively strong genetic impact on human life-span. However, many genetic factors and signaling pathways involved in ageing process are still unknown[26, 27]. Here we hypothesized that genes which control immune response to infectious disease are likely related with longevity, but immune-related genes usually present a very high polymorphism and thus are prone to be confounded by population stratification in regular case-control studies. For example, our former study suggested that a previously unrecognized population stratification, which was a geographic difference in genotype proportions and allele frequencies of the neck region of DC-SIGNR, existed in the Chinese of different geographic areas. However, in the present study, our peri-centenarian and control groups came from the same community, and were ethnically matched, thus the spurious association caused by population stratification could be ruled out. Furthermore, all participants shared the same living environment and common diet habits, thus non-genetic factors were averted to the greatest extent.
A total of 11 genotypes and 5 alleles in neck region tandem repeat of DC-SIGNR were investigated in this population. The distributions of genotype and allele frequencies were similar to previous reports on Han Chinese populations[11, 29]. Although there were reports suggesting a balanced selection could sharpen the alleles frequencies of this locus, the absence of association between the neck region polymorphism of DC-SIGNR and longevity in Han Chinese population may be caused by various reasons. First, most of the peri-centenarian individuals in this study had lower chance to get infectious diseases, as they were collected from Dujiangyan in Sichuan province, a relative isolated and remote region, i.e. the selection pressure on this locus was relaxed. For example, the sudden outbreak of SARS-CoV in 2003 was extensive in China but did not spread to this region [31, 32]. Furthermore, the HIV-1 case was rare in the region we studied. In this case, many infectious diseases could not exert selective pressure on such population. This may be one explanation as to why we could not find an association between the neck region polymorphism of DC-SIGNR and longevity.
Another explanation for our result might be the relative small sample size in our study, especially when the samples were stratified by gender, which is also a handicap in many association studies. Therefore, replication with a larger sample size may improve our understanding on the consequence of this polymorphism and provide more explorative data about the potential influence of this gene on human longevity. After all, it was equally likely that DC-SIGNR gene did not have a role in healthy aging of human beings.
We studied the association between VNTR polymorphism of DC-SIGNR neck region and longevity among peri-centenarian individuals in Han Chinese. Our results did not imply that the VNTR polymorphism was associated with longevity in our population. While, there was more 6/7 genotypes in female peri-centenarian group than that in female control group, with a marginal level of significance (5.56 vs. 1.28%, p = 0.041). The question of whether the DC-SIGNR could affect longevity in a gender-specific pattern remains unknown.
We are grateful to all the participants in this research. Also, we would like to acknowledge Shi-Fang Wu and Chun-Ling Zhu for their help in experiments. This work was supported by the Key State Research Program of China (2007CB507405), and Science and Technology Committee of Yunnan Province, and the National Natural Science Foundation of China, and the foundation of "Light in Western China" of the Chinese Academy of Sciences (to Q-PK).
- Soilleux EJ, Barten R, Trowsdale J: DC-SIGN; a related gene, DC-SIGNR; and CD23 form a cluster on 19p13. J Immunol. 2000, 165 (6): 2937-2942.View ArticlePubMedGoogle Scholar
- Geijtenbeek TB, Torensma R, van Vliet SJ, van Duijnhoven GC, Adema GJ, van Kooyk Y, Figdor CG: Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell. 2000, 100 (5): 575-585. 10.1016/S0092-8674(00)80693-5.View ArticlePubMedGoogle Scholar
- Lozach PY, Lortat-Jacob H, de Lacroix de Lavalette A, Staropoli I, Foung S, Amara A, Houles C, Fieschi F, Schwartz O, Virelizier JL, et al: DC-SIGN and L-SIGN are high affinity binding receptors for hepatitis C virus glycoprotein E2. J Biol Chem. 2003, 278 (22): 20358-20366. 10.1074/jbc.M301284200.View ArticlePubMedGoogle Scholar
- Gardner JP, Durso RJ, Arrigale RR, Donovan GP, Maddon PJ, Dragic T, Olson WC: L-SIGN (CD 209L) is a liver-specific capture receptor for hepatitis C virus. Proc Natl Acad Sci USA. 2003, 100 (8): 4498-4503. 10.1073/pnas.0831128100.View ArticlePubMedPubMed CentralGoogle Scholar
- Alvarez CP, Lasala F, Carrillo J, Muniz O, Corbi AL, Delgado R: C-type lectins DC-SIGN and L-SIGN mediate cellular entry by Ebola virus in cis and in trans. J Virol. 2002, 76 (13): 6841-6844. 10.1128/JVI.76.13.6841-6844.2002.View ArticlePubMedPubMed CentralGoogle Scholar
- Soilleux EJ, Morris LS, Rushbrook S, Lee B, Coleman N: Expression of human immunodeficiency virus (HIV)-binding lectin DC-SIGNR: Consequences for HIV infection and immunity. Hum Pathol. 2002, 33 (6): 652-659. 10.1053/hupa.2002.124036.View ArticlePubMedGoogle Scholar
- Soilleux EJ, Morris LS, Leslie G, Chehimi J, Luo Q, Levroney E, Trowsdale J, Montaner LJ, Doms RW, Weissman D, et al: Constitutive and induced expression of DC-SIGN on dendritic cell and macrophage subpopulations in situ and in vitro. J Leukoc Biol. 2002, 71 (3): 445-457.PubMedGoogle Scholar
- Bashirova AA, Geijtenbeek TB, van Duijnhoven GC, van Vliet SJ, Eilering JB, Martin MP, Wu L, Martin TD, Viebig N, Knolle PA, et al: A dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein is highly expressed on human liver sinusoidal endothelial cells and promotes HIV-1 infection. J Exp Med. 2001, 193 (6): 671-678. 10.1084/jem.193.6.671.View ArticlePubMedPubMed CentralGoogle Scholar
- Mitchell DA, Fadden AJ, Drickamer K: A novel mechanism of carbohydrate recognition by the C-type lectins DC-SIGN and DC-SIGNR. Subunit organization and binding to multivalent ligands. J Biol Chem. 2001, 276 (31): 28939-28945. 10.1074/jbc.M104565200.View ArticlePubMedGoogle Scholar
- Feinberg H, Guo Y, Mitchell DA, Drickamer K, Weis WI: Extended neck regions stabilize tetramers of the receptors DC-SIGN and DC-SIGNR. J Biol Chem. 2005, 280 (2): 1327-1335. 10.1074/jbc.M409925200.View ArticlePubMedGoogle Scholar
- Barreiro LB, Quintana-Murci L: DC-SIGNR neck-region polymorphisms and HIV-1 susceptibility: From population stratification to a possible advantage of the 7/5 heterozygous genotype. J Infect Dis. 2006, 194 (8): 1184-1185. 10.1086/507845. author reply 1185-1187View ArticlePubMedGoogle Scholar
- Chan VS, Chan KY, Chen Y, Poon LL, Cheung AN, Zheng B, Chan KH, Mak W, Ngan HY, Xu X, et al: Homozygous L-SIGN (CLEC4M) plays a protective role in SARS coronavirus infection. Nat Genet. 2006, 38 (1): 38-46. 10.1038/ng1698.View ArticlePubMedGoogle Scholar
- Lichterfeld M, Nischalke HD, van Lunzen J, Sohne J, Schmeisser N, Woitas R, Sauerbruch T, Rockstroh JK, Spengler U: The tandem-repeat polymorphism of the DC-SIGNR gene does not affect the susceptibility to HIV infection and the progression to AIDS. Clin Immunol. 2003, 107 (1): 55-59. 10.1016/S1521-6616(02)00050-5.View ArticlePubMedGoogle Scholar
- Liu H, Carrington M, Wang C, Holte S, Lee J, Greene B, Hladik F, Koelle DM, Wald A, Kurosawa K, et al: Repeat-region polymorphisms in the gene for the dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-related molecule: effects on HIV-1 susceptibility. J Infect Dis. 2006, 193 (5): 698-702. 10.1086/499820.View ArticlePubMedGoogle Scholar
- Nattermann J, Ahlenstiel G, Berg T, Feldmann G, Nischalke HD, Muller T, Rockstroh J, Woitas R, Sauerbruch T, Spengler U: The tandem-repeat polymorphism of the DC-SIGNR gene in HCV infection. J Viral Hepat. 2006, 13 (1): 42-46. 10.1111/j.1365-2893.2005.00652.x.View ArticlePubMedGoogle Scholar
- Barreiro LB, Neyrolles O, Babb CL, van Helden PD, Gicquel B, Hoal EG, Quintana-Murci L: Length variation of DC-SIGN and L-SIGN neck-region has no impact on tuberculosis susceptibility. Hum Immunol. 2007, 68 (2): 106-112. 10.1016/j.humimm.2006.10.020.View ArticlePubMedGoogle Scholar
- Boily-Larouche G, Zijenah LS, Mbizvo M, Ward BJ, Roger M: DC-SIGN and DC-SIGNR genetic diversity among different ethnic populations: potential implications for pathogen recognition and disease susceptibility. Hum Immunol. 2007, 68 (6): 523-530. 10.1016/j.humimm.2007.02.002.View ArticlePubMedGoogle Scholar
- Li H, Tang NL, Chan PK, Wang CY, Hui DS, Luk C, Kwok R, Huang W, Sung JJ, Kong QP, et al: Polymorphisms in the C-type lectin genes cluster in chromosome 19 and predisposition to severe acute respiratory syndrome coronavirus (SARS-CoV) infection. J Med Genet. 2008, 45 (11): 752-758. 10.1136/jmg.2008.058966.View ArticlePubMedGoogle Scholar
- Temple SE, Cheong KY, Almeida CM, Price P, Waterer GW: Polymorphisms in lymphotoxin alpha and CD14 genes influence TNFalpha production induced by Gram-positive and Gram-negative bacteria. Genes Immun. 2003, 4 (4): 283-288. 10.1038/sj.gene.6363963.View ArticlePubMedGoogle Scholar
- Pawelec G: Immunity and ageing in man. Exp Gerontol. 2006, 41 (12): 1239-1242. 10.1016/j.exger.2006.09.005.View ArticlePubMedGoogle Scholar
- Xie L, Gong YY, Lian SG, Yang J, Yang Y, Gao SJ, Xu LY, Zhang YP: Absence of association between SNPs in the promoter region of the insulin-like growth factor 1 (IGF-1) gene and longevity in the Han Chinese population. Exp Gerontol. 2008, 43 (10): 962-965. 10.1016/j.exger.2008.08.004.View ArticlePubMedGoogle Scholar
- Promislow DEL: Costs of Sexual Selection in Natural-Populations of Mammals. P Roy Soc Lond B Bio. 1992, 247 (1320): 203-210. 10.1098/rspb.1992.0030.View ArticleGoogle Scholar
- Maklakov AA, Fricke C, Arnqvist G: Sexual selection affects lifespan and aging in the seed beetle. Aging Cell. 2007, 6 (6): 739-744. 10.1111/j.1474-9726.2007.00333.x.View ArticlePubMedGoogle Scholar
- Liker A, Szekely T: Mortality costs of sexual selection and parental care in natural populations of birds. Evolution. 2005, 59 (4): 890-897.View ArticlePubMedGoogle Scholar
- Mitchell BD, Hsueh WC, King TM, Pollin TI, Sorkin J, Agarwala R, Schaffer AA, Shuldiner AR: Heritability of life span in the Old Order Amish. Am J Med Genet. 2001, 102 (4): 346-352. 10.1002/ajmg.1483.View ArticlePubMedGoogle Scholar
- Bartke A: Impact of reduced insulin-like growth factor-1/insulin signaling on aging in mammals: novel findings. Aging Cell. 2008, 7 (3): 285-290. 10.1111/j.1474-9726.2008.00387.x.View ArticlePubMedGoogle Scholar
- Kenyon C: A conserved regulatory system for aging. Cell. 2001, 105 (2): 165-168. 10.1016/S0092-8674(01)00306-3.View ArticlePubMedGoogle Scholar
- Thomas DC, Witte JS: Point: population stratification: a problem for case-control studies of candidate-gene associations?. Cancer Epidemiol Biomarkers Prev. 2002, 11 (6): 505-512.PubMedGoogle Scholar
- Wang H, Hu YD, Wang CH, Dong XM, Li LX, Liu FP, Yan LB, Zhou P, Tong XD, Shi LL, et al: [Analysis of DC-SIGN and DC-SIGNR genetic polymorphism in Chinese Han population]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006, 23 (4): 466-469.PubMedGoogle Scholar
- Barreiro LB, Patin E, Neyrolles O, Cann HM, Gicquel B, Quintana-Murci L: The heritage of pathogen pressures and ancient demography in the human innate-immunity CD209/CD209L region. American journal of human genetics. 2005, 77 (5): 869-886. 10.1086/497613.View ArticlePubMedPubMed CentralGoogle Scholar
- Peiris JS, Yuen KY, Osterhaus AD, Stohr K: The severe acute respiratory syndrome. N Engl J Med. 2003, 349 (25): 2431-2441. 10.1056/NEJMra032498.View ArticlePubMedGoogle Scholar
- Tsang KW, Ho PL, Ooi GC, Yee WK, Wang T, Chan-Yeung M, Lam WK, Seto WH, Yam LY, Cheung TM, et al: A cluster of cases of severe acute respiratory syndrome in Hong Kong. N Engl J Med. 2003, 348 (20): 1977-1985. 10.1056/NEJMoa030666.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2350/10/134/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.