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Table 1 Primers for PCR and DNA sequencing for detection of IDS mutations

From: The mutational spectrum of hunter syndrome reveals correlation between biochemical and clinical profiles in Tunisian patients

PrimerSequence 5′ > 3′Tm (°C)Expected products (bp)
F1-IDSGAGGAGGTCTCTGTGGCTGC63 .5376
R1-IDSAGGGACGGTAGGAAGGAGTG61 .4
F2-IDSCACTCACTATCTCGCTTCCTC59.8540
R2-IDSCCTCTAACAAGATGTCCCG56.7
F3-IDSGGTTACCTAAGAGATGGCAG57.3542
R3-IDSCAGCCTGTGTCCTCCCTAC61.0
F4-IDSGTAGATGAGGAAACTGAGCC57.3475
R4-IDSCTATTCAATGAGTCTGACACG55.9
F5-IDSGCCTGGAAAACAAGAAACACC57.9487
R5-IDSTGGCGATGGCAGGATGTAG58.8
F6-IDSAGGCAGGAGGTGGGGACAG63.1607
R6-IDSCCAGCACTTTGCCTGATAACTC60.3
F7-IDSCTAAGGGGTAGGGATTGGGAG61.8440
R7-IDSACCCACACCTATCCGTCAAGC61.8
F8-IDSGGTGATGAGTTTCTACTTCCT55.9465
R8-IDSGAGATGTTCAGAAAGCGTG54.5
F9-IDSGTGAGGTGCCGAGGTGGTG63.1468
R9-IDSGGTGCGTATGGAATAGCCC58.8
F9–1-IDSCTTCAGACATCCCTCAGTGG95.4283
R9–1-IDSGCTCTAACTCCTCCTCTCACC61.8