Table 1 Primers for PCR and DNA sequencing for detection of IDS mutations
Primer | Sequence 5′ > 3′ | Tm (°C) | Expected products (bp) |
|---|---|---|---|
F1-IDS | GAGGAGGTCTCTGTGGCTGC | 63 .5 | 376 |
R1-IDS | AGGGACGGTAGGAAGGAGTG | 61 .4 | |
F2-IDS | CACTCACTATCTCGCTTCCTC | 59.8 | 540 |
R2-IDS | CCTCTAACAAGATGTCCCG | 56.7 | |
F3-IDS | GGTTACCTAAGAGATGGCAG | 57.3 | 542 |
R3-IDS | CAGCCTGTGTCCTCCCTAC | 61.0 | |
F4-IDS | GTAGATGAGGAAACTGAGCC | 57.3 | 475 |
R4-IDS | CTATTCAATGAGTCTGACACG | 55.9 | |
F5-IDS | GCCTGGAAAACAAGAAACACC | 57.9 | 487 |
R5-IDS | TGGCGATGGCAGGATGTAG | 58.8 | |
F6-IDS | AGGCAGGAGGTGGGGACAG | 63.1 | 607 |
R6-IDS | CCAGCACTTTGCCTGATAACTC | 60.3 | |
F7-IDS | CTAAGGGGTAGGGATTGGGAG | 61.8 | 440 |
R7-IDS | ACCCACACCTATCCGTCAAGC | 61.8 | |
F8-IDS | GGTGATGAGTTTCTACTTCCT | 55.9 | 465 |
R8-IDS | GAGATGTTCAGAAAGCGTG | 54.5 | |
F9-IDS | GTGAGGTGCCGAGGTGGTG | 63.1 | 468 |
R9-IDS | GGTGCGTATGGAATAGCCC | 58.8 | |
F9–1-IDS | CTTCAGACATCCCTCAGTGG | 95.4 | 283 |
R9–1-IDS | GCTCTAACTCCTCCTCTCACC | 61.8 |