Table 1 Primers sequences used for SNPs genotyping in the OPG gene
SNP | Sequence 5′- 3′ | PCR-Product (bp) | Genotyping Method |
|---|---|---|---|
rs3102734 | Forward: TGCCGGGACGCTATATATAAC | 226 | Restiction digestion (HAEIII) |
Reverse: TTCTCCCCGCCGGTCCGCT | |||
rs2073618 | Forward 1: CGGGGACCACAATGAACAAC | 273 | Allele specifc polymerase chain reaction or ASPCR |
Forward 2: CGGGGACCACAATGAACAAG | |||
Reverse: CTCTCTCTTGCTGTCTTCCAT | |||
rs2228568 rs7844539 | Forward: TTACAAGGAAACTGGGGAGC | 437 | Sanger Sequencing |
Reverse: TGTGTGAGGAGGCATTCTTC |