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Table 1 Oligonucleotide primers used for site-directed mutagenesis in generation of GAA constructs

From: Clinical course, mutations and its functional characteristics of infantile-onset Pompe disease in Thailand

Primer Sequence
A261T-F 5’-ACAGGCCTCACCGAGCACCTC-3’
A261T-R 5’-GAGGTGCTCGGTGAGGCCTGT-3’
Y292X-F 5’-GCGAACCTCTAGGGGTCTCACCC-3’
Y292X-R 5’-GGGTGAGACCCCTAGAGGTTCGC-3’
D513G-F 5’-CAGGTGCCCTTCGGCGGCATGTGGATTG-3’
D513G-R 5’-CAATCCACATGCCGCCGAAGGGACCTG-3’
L632R-F 5’-GCCAGAAATCCGGCAGTTTAACC-3’
L632R-R 5’-GGTTAAACTGCCGGAATTTCTGGC-3’