Table 1 Oligonucleotide primers used for site-directed mutagenesis in generation of GAA constructs
Primer | Sequence |
|---|---|
A261T-F | 5’-ACAGGCCTCACCGAGCACCTC-3’ |
A261T-R | 5’-GAGGTGCTCGGTGAGGCCTGT-3’ |
Y292X-F | 5’-GCGAACCTCTAGGGGTCTCACCC-3’ |
Y292X-R | 5’-GGGTGAGACCCCTAGAGGTTCGC-3’ |
D513G-F | 5’-CAGGTGCCCTTCGGCGGCATGTGGATTG-3’ |
D513G-R | 5’-CAATCCACATGCCGCCGAAGGGACCTG-3’ |
L632R-F | 5’-GCCAGAAATCCGGCAGTTTAACC-3’ |
L632R-R | 5’-GGTTAAACTGCCGGAATTTCTGGC-3’ |