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Table 2 PCR condition for HPV detection with SPF primers set

From: Lack of association between methylenetetrahydrofolate reductase C677T polymorphism, HPV infection and cervical intraepithelial neoplasia in Brazilian women

Number of cycles

Temperature (°C)

Time (minutes)

Stage

1

94

1

Denaturation

40

94

1

Denaturation

45

1

Annealing

72

1

Extension

1

72

5

Final Extension

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BMC Medical Genetics

ISSN: 1471-2350

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