Fig. 3

DNA sites with variable methylation under hypoxia. a The top 1000 most variable CpG probes were used to perform hierarchical clustering based on Euclidean distances. The analysis well separated samples into four clusters grouping all the replicates of each experimental condition. b Overall distribution of hyper (Δβ ≥ 0.2) and hypo-methylated (Δβ ≤ − 0.2) probes of “Hypoxia probes” (shCTR HYP vs shCTR NX). and “HIF1A probes” (shHIF1A HYP vs shCTR HYP) c Distribution of “Hypoxia probes” and “HIF1A probes” in relation to CpG-centric annotation. d Distribution of “Hypoxia probes” and “HIF1A probes” to Gene-centric annotation. e Plot showing the inverse methylation status (β values) of 150 probes (common probes) commonly methylated in “Hypoxia probes” and “HIF1A probes”. f Gene-centric distribution of hyper (Δβ ≥ 0.2) and hypo-methylated (Δβ ≤ − 0.2) common probes