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Fig. 4 | BMC Medical Genetics

Fig. 4

From: A functional variant alters binding of activating protein 1 regulating expression of FGF7 gene associated with chronic obstructive pulmonary disease

Fig. 4

The regulatory element containing the rs12905203 variant demonstrates enhancer activity. a and b Sequences carrying risk (G) and non-risk (a) variants were cloned upstream of a minimal thymidine kinase promoter luciferase construct to measure luciferase activation following transient transfection and stimulation with PMA/Ionomycin. c and d The luciferase activity assay vectors were co-transfected with constructs expressing AP-1 transcription factors. Seventy-two hours post-transfection, luciferase activity for risk and non-risk variants was measured and compared to the control. The statistical differences between risk and non-risk alleles of the variant were calculated using Student’s t-test

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BMC Medical Genetics

ISSN: 1471-2350

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