Fig. 1

PCR-RFLP analysis for Factor-V Leiden G1691A polymorphism. Two fragments of 47 bp and 159 bp indicate mutant homozygous (A/A), three fragments of 36 bp, 47 bp and 123 bp for wild type homozygous (G/G) and four fragments of 36 bp, 47 bp, 123 bp and 159 bp indicate A/G genotype. Lane L show the 100 bp ladder; Lane 1 and 2 undigested PCR products. Lane 3 and 4 indicate wild type homozygous (G/G); 5, 7–9 homozygous mutant (A/A) and Lane 6 represents heterozygous (A/G) genotype