Table 1 Primer sequences and amplicon sizes for GLA gene sequencing
Exon | Sense primer | Anti-sense primer | Amplicon size (bp) |
|---|---|---|---|
GLA-E1 | 5’ CGTGACTGATTATTGGTCTACCTCTG 3’ | 5’ CGTTGAGACTCTCCAGTTCCCC 3’ | 433 |
GLA-E2 | 5’ TAACGGGATAAGAGAGACAAAAGAAA 3’ | 5’ AACTCTTGACCTCAGGTGATCCA 3’ | 521 |
GLA-E3 | 5’ AATACCTGGTGAAGTAACCTTGTCTC 3’ | 5’ CTTTCCTTTGTGGCTAAATCTCTG 3’ | 329 |
GLA-E4 | 5’ CCCTGGATGACAGACTGAACCC 3’ | 5’ GGAGACCTTGGTTTCCTTTGTTGT 3’ | 293 |
GLA-E5 | 5’ TCAATCTGTAAACTCAAGAGAAGGC 3’ | 5’ TCACATAAAGCCTCCTCCCAG 3’ | 375 |
GLA-E6 | 5’ CAGGATGCTGTGGAAAGTGGT 3’ | 5’ AAGCACTTGTAGGAAAAATTAAAATGA 3’ | 480 |
GLA-E7 | 5’ TTTTTCCTACAAGTGCTTGATAGTTCT 3’ | 5’ ACCTCAGGTGATCTGCCCG 3’ | 529 |