Fig. 2

PHACTR1 transcripts differential expression in human tissues and cell types. We designed transcript-specific primers and amplified PHACTR1 by RT-PCR. The PCR fragment for the long transcript is 562-bp. We did not amplify a 769-bp fragment using this set of primers, indicating that exon 10.11 is absent from the long transcript. The PCR fragments for the intermediate transcripts A+, A-, B+ and B- are 880-bp, 673-bp, 739-bp and 532-bp, respectively. The PCR fragment for the short transcript is 185-bp. HUVEC: human umbilical vein endothelial cell; teloHAEC: immortalized human aortic endothelial cells; HCASMC: human coronary artery smooth muscle cells; HAEC: human aortic endothelial cells; HCAEC: human coronary artery endothelial cells; HASMC: human aortic smooth muscle cells; Mono: monocytes; PMA: phorbol myristate acetate; LPS: lipopolysaccharide