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Fig. 4 | BMC Medical Genetics

Fig. 4

From: Polymorphisms at the F12 and KLKB1 loci have significant trait association with activation of the renin-angiotensin system

Fig. 4

Generation of Ang I from renin substrate tetradecapeptide (pre-angiotensinogen 1–14) by KAL-activated pro-renin. a AGT was incubated with KAL (upper panel) and with pro-renin and KAL (lower panel) as described in methods. The reaction digests were purified through ZipTip and subjected to MALDI-TOF analysis. b TOF/TOF analysis of the precursor mass 1759.9 and 1296.81 c Quantification of the MS scans. The % conversion of AGT (1–14 to 1–10) was quantified from the relative intensity of the Ang I peptide under different experimental conditions

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BMC Medical Genetics

ISSN: 1471-2350

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