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Fig. 3 | BMC Medical Genetics

Fig. 3

From: Development and validation of a high throughput, closed tube method for the determination of haemoglobin alpha gene (HBA1 and HBA2) numbers by gene ratio assay copy enumeration-PCR (GRACE-PCR)

Fig. 3

Normalized ratios of -dF/dT peak heights for different copy numbers of the HBA1 and HBA2 genes. The horizontal bars indicate the mean ratio and the vertical bars indicate the range from the minimum to maximum ratio observed for each gene copy number. The data was normalized with the equation: Normalized ratio = (RWT Control/TWT Control)/(RSample/TSample), where R and T is reference gene and target gene peak heights, respectively

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