Fig. 4

Luciferase expression results from transfected K562 and 293 T cells. a Schematic illustration of luciferase reporter constructs for each SNP. b Luciferase reporter constructs containing ancestral alleles (rs2855125-A, rs2855126-G, rs11036415-G, rs11036496-G, rs4320977-T, and rs4348933-T) or derived alleles (rs2855125-C, rs2855126-C, rs11036415-T, rs11036496-C, rs4320977-C, and rs4348933-C) were transiently transfected into K562 or 293 T cells. Cell lysates were analyzed for firefly and Renilla luciferase activity and the ratio of firefly/Renilla luciferase fluorescence was calculated. Data represent the average ± one SD from three replicates, * p ≤ 0.05.