BRAFmutation analysis of the primary tumor. (a) Direct sequencing chromatographs of the BRAF VK600–601E mutation demonstrating triplet deletion from T1799 to A1801. (b) Schematic representation of the BRAF protein structure and various mutations. CR-1, −2, and −3 represent conserved regions. The kinase activation segment is located between codons 594 and 623. Modified from Wan PT et al. .