Figure 4From: Detection of Catalase as a major protein target of the lipid peroxidation product 4-HNE and the lack of its genetic association as a risk factor in SLE The mass spectrum showing the tryptic fragments of the 80 kD band analyzed by MALDI-TOF-MS that matched to sequences from catalase. The Coomassie stained band migrating at 80 kD was excised and subjected to in-gel tryptic digestion and MALDI-TOF-MS. The mass of each individual peak was used to perform a Mascot Search, which identified human catalase as the primary candidate antigen (38% match). Of the 59 mass values searched, 19 of them matched to sequences obtained from catalase. The probability based Mowse score obtained for the Mascot search analysis was 100, where a score of 64 is significant (p < 0.05). X-axis gives the mass to charge ratio (m/z) and Y-axis gives the intensity of the peaks in counts.Back to article page