Figure 2
From: Association between Ngb polymorphisms and ischemic stroke in the Southern Chinese Han population
Mismatched PCR-RFLP analysis of the 89+104 c>t polymorphism. KpnI recognition site was created in the PCR product by means of mismatched primers KpnINgb. After cleavage with KpnI, the cc individuals (lane 5 and 6) carried a 260 bp fragment, the tt individuals (lane 1 and 2) carried a 284 bp fragment, and the ct individuals (lane 3 and 4) carried both 260 bp and 284 bp fragments. The size marker (M) is D2000 ranging from 100 bp to 2000 bp.