Figure 3

RT-PCR detection of SOD2 mRNAs. Primer sets were designed RT-PCR product spanning exon 2 through 5 (2–3–4–5), exon 3 through 5 (3–4–5), exon 4 to 5 (4–5) and exon 3 to 5 deleting 4 (3–5). Each set are tested in two T10/T10 homozygotes (first 2 lanes) and three T10/T9 heterozygotes (next 3 lanes) at three different annealing temperatures, 55°C, 58°C, and 60°C. Bottom panel shows the RT-PCR products with the primer sets that detect exon 4–5 only, 3–5 only and the combination of 4–5 plus 3–5.