Screening of the transcriptional regulatory regions of vascular endothelial growth factor receptor 2 (VEGFR2) in amyotrophic lateral sclerosis

Table 1 Primers used to amplify regulatory and exonic regions of the VEGFR2 gene, and polymorphisms present.

	Polymorphisms	Amino acid change	SNP reference	Forward primer	Reverse primer	Annealing temperature
Promoter	-305 C/T	n/a	Novel	GCGACCCGGCATACTTG	AGGCGGCCCGGGTCTCCAC	55
	-263 C/T		Novel
	-123 C/G		Rs 10027862
	-65 C/T		Rs 9994560
	-57 C/T		Novel
	-17 A/T		Rs 6824124
5' UTR	+31 G/A		Rs 7667298
Exon 7	+1192 G/A	Val to Ile	Rs 2305948	CTGGTGTCCCTGTTTTTAGCAT	GTATATCAGCACATCTCATCTTTA	50
Exon 18	+2846 T/A	Val to Glu	Rs 1139776	TAATTAAGCCAGGACAAAGGAGTA	CTAGTAGGCCCACATAAGCACA	50
Exon 21	+3157 T/C	Val to Ile	Rs 13129474	TTCAATTATCTCCATGGTTTACTA	TCACTTTTGGGGAGACAGAAT	46
Exon 27	+3931 C/G	Pro to Ala	Rs 11540507	CACCCTATGTAGCCAAGAAGTC	CTACAGATGGGAGGAAGCACAC	53

SNP reference obtained from ensembl database for known polymorphisms; Novel polymorphisms are those identified in this study. Primers are listed 5' to 3'

ISSN: 1471-2350