Arrhythmogenic right ventricular cardiomyopathy type 6 (ARVC6): support for the locus assignment, narrowing of the critical region and mutation screening of three candidate genes

Matolweni, Luzuko O; Bardien, Soraya; Rebello, George; Oppon, Ekow; Munclinger, Miroslav; Ramesar, Rajkumar; Watkins, Hugh; Mayosi, Bongani M

doi:10.1186/1471-2350-7-29

Table 1 Oligonucleotide primers used for PCR amplification and DNA sequencing of the exons of the ITGA8, FRMD4A, and LAMR1P6 genes.

From: Arrhythmogenic right ventricular cardiomyopathy type 6 (ARVC6): support for the locus assignment, narrowing of the critical region and mutation screening of three candidate genes

Gene	Exon No.	PCR fragment size (bp)	Forward primer (5' → 3')	Reverse primer (5' → 3')	Melting temperature (°C)
ITGA8	1	587	acc gcc aga ttc cac cag	tgggtc ttc tgg gtc ctg t	58
	2	300	ggg cag gag gtt aca gag ac	acc aag aca gcg gga agt c	56
	3	300	gag gag ttt gac aag gat tgg	gct ttc cta aac atg agc ttg	54
	4	368	ggc caa tta att cac cca at	tac cta ccc ccg aag ctt tt	48
	5	399	tca caa cgc tat ttg gtg taa ga	cag gct agg tat gtt cct caa ga	58
	6	223	tct ctc aag gga gag agt tca a	tgt ttg agg tga tgg tta tgc t	45
	7	296	ttt caa ggg aat gtg ggt ct	cat cgg ata agt cag gga ta	54
	8	249	tgg tag ccc ttt cac ctt tt	ctc cat taa tat ccc ttt gg	56
	9	250	ata tgg cgt gtg tgt gtg tg	cgg tgg att agg tgt att aag tg	54
	10	238	cca att cta tgg caa atg gt	tgc cta gga cag ttt cca gt	56
	11	329	att cac tgg gtc agg ggt tag	tga ggc aat aag gaa ggg tgt	58
	12	464	taa caa agc agc gaa agc aa	taa ctg cac cca cac aca cc	58
	13	475	tag aga agt tga ggc agg agg atc act tg	gct tca gcc tca tct gta gaa gaa aac tgc	56
	14	250	acc atc agc atg tag ggg ta	ccc cag aat aaa tcg ttg ga	55
	15	243	Atg cag tga agt ggg ctt ct	caa gat acc cag gtg atg tga a	55
	16	553	ttg gta gaa ggg gtg tgt g	gca tga taa atg agc tgg aa	56
	17	468	ttc cag ctc att tat cat gc	gaa gtc agg gtc aag agg ag	55
	18	399	aca taa atc agc ctc cat cta agc	cat gca gaa gta aaa atg aaa gga	56
	19	278	ttt gtg gca cca ttc aga att t	caa gaa ttt cac gca gca tag tt	56
	20	460	gat gat tct ctt gcc tct tcc tac	gtg gct cat tgc tgt tat gat tta	56
	21	299	ctc ctg ggt gtt tga gat cc	agg tgc tga gtt gct gag tg	58
	22	244	ggg ttt tgc agg aat gac tt	aag ggc ctt gac aga tga c	58
	23	268	cgt gtg ttc tcc tgt gat atg	cat cag gaa aat tcc aca tca	55
	24	324	gaa aac cac ttt ctt ctg ctt c	cac agc gag atc ctg tct ca	56
	25	396	tgc cat atc gac ttt gaa tt	aat caa caa agc cac tga	55
	26	369	cac agg tct gga tcc tca tgt	tga gca ctg aag gag acc aa	58
	27	298	ggt gat gac ttt tga gga aat ga	cgc tat gaa aag act tcc cac t	58
	28	263	att agg tgg ctg cca gta taa c	acc act aaa gcc tag cac aag c	56
	29	380	gga tcc ttt cag atc aac ttc c	gca aac aag aca cga ttt tcc	56
	30	365	ctg ggg aat aga gag cga gac	gtg cgg tgt aga tga ggt gat	58
FRMD4A	1	597	acc tcc tat gcc tcc ctg aa	ctg gca gca att ctt cct tt	58
	2	371	cac agt tcc cac cct gtc tt	aaa gag cct gcg gga taa ag	58
	3	395	ata cag tgc cga ctc cca ga	gga tgc ctt agg ctg gtg ta	58
	4	387	gac cct tga ctt ggc tgt gt	caa gtg gtc tgc agc tga tt	58
FRMD4A splice variant	1	688	ggt gag cat gtg acc tac tgt	cca act gtt cag gaa acg tg	58
	nested primers		gc gtg act tgc atg atg tga c	ccc aag cct tat agc act aaa c
LAMR1P6	1	884	ttc cca tcg caa ctt aaa gg	caa cgt tgt ttc cat gtt gc	58
	nested primers		aca gag caa tgg tgg gaa ag	ctt tcc cac cat tgc tct gt
	nested primers		aat tgc tgg cca ctt cac tc	caa ctg cat caa acc cac tg

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