Figure 6

Validation of strong over-expression of GART and moderate over-expression of SH3BGR mRNA in mid-gestation fetal hearts with +21 by northern blotting and real-time RT-PCR. A, northern blot containing total RNA from control and +21 fetal hearts at 17 to 19 weeks gestation (3.0 to 3.5 cm heel-toe length) was hybridized with the indicated cDNA probes, with stripping of the blot between hybridizations. Arrows indicate the specific transcripts, and the dashes show positions of the 18S ribosomal RNA as a size marker. The ratio of GART and SH3BGR signals to that obtained with the GAPDH probe, as measured by Phosphorimaging, is shown below each lane. B, real-time RT-PCR analysis of GART mRNA in control and +21 fetal hearts. The values for GART mRNA were normalized to HPRT expression in each sample. Stages of development (heel-toe length) are color-coded.