The host response to the probiotic Escherichia coli strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1

Table 3 EcN specific expression of selected genes in Caco-2 cells cocultured with inactivated bacteria or bacteria conditioned media for 6 hours.

Gene	Inactivated EcN	EcN CM
DUSP5	(+)	no EcN specific gene regulation
ELF3	(+)	no EcN specific gene regulation
MCP-1	no EcN specific gene regulation	no EcN specific gene regulation
MIP-2α	(+++)	no EcN specific gene regulation
MIP-2β	(++)	no EcN specific gene regulation
NFκBIA	no EcN specific gene regulation	no EcN specific gene regulation

Gene expression was analyzed by real-time RT-PCR and differences of fold changes for EcN and E. coli MG1655 were compared. EcN specific fold change of Caco-2 cell gene expression (+) between 0.5 and 2, (++) between 2 and 10 and (+++) > 10.

ISSN: 1471-2350