Table 1 Forward and reverse PCR primers used for amplification of the coding and promoter regions of PRX-IV
From: Mutational analysis of Peroxiredoxin IV: exclusion of a positional candidate for multinodular goitre
Exon number | Exon size (bp) | cDNA position | Primer sequence | PCR size |
|---|---|---|---|---|
1 | 241 | 1 | F taggtgagaaccaggcccgcccagg R gtcgtgcacgcggttgtagctgc | 457 |
2 | 118 | 242 | F ataatttgtttgatactcctg R ctgccaaagagatctaaaggtagc | 307 |
3 | 117 | 360 | F gctattcctgaattgtctg R acagtcttttagactactcc | 396 |
4 | 123 | 477 | F ttgagctaccgtgcctggc R aggtaagcgttctgtttg | 281 |
5 | 131 | 608 | F gtaaataatcagaaaactttgg R gcatccttttgtgtggatgg | 326 |
6 | 35 | 643 | Fctcctgacctcgtgatctgc R gctgaaggtaactgaattctg | 226 |
7 | 48 | 691 | F tttggcatcagtgtctaacgc R tttagtcaaggcatgggc | 298 |
cDNA | Â | 13 | F caagggacgtgtttctgcgc R cagctggatctgggattatc | 815 |
Promoter region | Â | -936 | F gctgtctttgaaacaatag R tgtatacattcgtgggatgc | 475 |