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Table 3 Comparisons of GeneChip® and RT-PCR expression fold changes in mutant versus wild-type MECP2 expressing clones for 11 differentially expressed genes

From: Gene expression patterns vary in clonal cell cultures from Rett syndrome females with eight different MECP2 mutations

 

Fold Changes for Paired Comparisons

Gene Symbol/Unigene No.

Method

705delG

R306C

1155del32

R106W

p value

INCREASED

      

MMP3

GC

0.91

5.80

31.50

3.90

0.113

 

RT

0.89

4.81

11.63

3.49

0.094

FBLN1

GC

2.00

11.80

2.00

0.37

0.228

 

RT

2.03

15.87

2.97

0.72

0.199

SLC16A7

GC

1.80

1.40

2.30

0.77

0.210

 

RT

1.30

1.69

1.76

0.94

0.112

MTAP44

GC

1.00

2.40

6.10

3.50

0.081

 

RT

1.36

4.77

20.10

24.15

0.058

LTBP1

GC

1.20

1.30

2.10

2.00

0.047

 

RT

1.36

3.25

3.59

2.16

0.029

NTN4

GC

3.20

5.50

15.10

3.80

0.016

 

RT

2.30

2.10

9.80

3.10

0.039

Hs.19669

GC

2.00

2.20

1.90

1.40

0.008

 

RT

1.22

1.60

2.07

1.30

0.040

Hs.23311

GC

5.90

0.12

2.60

4.40

0.609

 

RT

3.00

0.23

1.86

11.79

0.467

DECREASED

      

COMP

GC

0.28

0.37

0.02

0.10

0.045

 

RT

0.18

0.25

0.05

0.09

0.011

KLF5

GC

0.53

0.42

0.56

0.27

0.014

 

RT

0.63

0.29

1.25

0.42

0.157

CRIP1

GC

0.32

2.80

0.11

0.50

0.347

 

RT

0.20

2.81

0.10

0.42

0.284

  1. P-values correspond to t-tests of whether average log fold change across the 4 different sets of matched clones differs from zero GC = GeneChip, RT = RT-PCR