Transcriptional activation of luciferase reporter constructs by the wild-type and mutant TBX1. (A) Schematic diagram of 4XT/2-minP reporter construct. Four conserved T-half sites “ATTTCACACCT” were oriented head to tail, synthesised and subcloned into the KpnI − HindIII sites in the pGL4.25 [luc2CP/minP] plasmid. (B) and (C) show that the 293 T or COS7 cells were co-transfected with the 4XT/2-minP reporter construct containing the T-box binding elements and either a pcDNA3.1 (+) control vector (Blank), the TBX1 wild-type construct, or the mutant constructs. The results were normalized for transfection efficiency to a co-transfected renilla luciferase vector and are shown as the mean ± SEM of three independent experiments performed in triplicate.