Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes

BMC Medical Genetics

Table 4 Correlations between polymorphisms in DNA repair genes, comet assay and BMCyt assay in COPD patients

Parameters	XRCC1		OGG1		XRCC3
	Risk allele		Risk allele		Risk allele
	(ArgGln+GlnGln)		(SerCys+CysCys)		(ThrMet+MetMet)
	Spearman’s rho	p value	Spearman’s rho	p value	Spearman’s rho	p value
% Residual damage- basal DI in alkaline comet assay	−0.497	0.007	NS	NS	NS	NS
% Residual damage- basal DI in neutral comet assay	−0.495	0.007	NS	NS	NS	NS
% Residual damage- BI	NS	NS	NS	NS	0.350	0.036
% Residual damage- KR	NS	NS	NS	NS	0.444	0.007
FEV₁- basal DI in alkaline comet assay	NS	NS	NS	NS	0.336	0.045
FEV₁₋ CC	−0.404	0.029	NS	NS	NS	NS
FEV₁₋ KR	−0.398	0.044	NS	NS	NS	NS
FEV₁/ FVC- KR	−0.441	0.024	NS	NS	−0.408	0.013
FEV₁/ FVC- BUD	NS	NS	NS	NS	−0.357	0.032
FEV₁/ FVC- BI	NS	NS	NS	NS	−0.328	0.051
FVC- CC	−0.428	0.029	−0.645	0.024	−0.343	0.041
FVC- basal DI in alkaline comet assay	NS	NS	NS	NS	0.423	0.010
PY- basal DI in neutral comet assay	NS	NS	−0.643	0.024	NS	NS
PY- basal DI in alkaline comet assay	NS	NS	−0.616	0.033	NS	NS
Basal cell- CC	0.442	0.032	NS	NS	NS	NS
Basal cell- MN	NS	NS	NS	NS	0.381	0.022
Basal cell- BUD	NS	NS	NS	NS	0.467	0.004
Basal cell- KL	NS	NS	−0.666	0.018	−0.359	0.032

FEV ₁, forced expiratory volume in one second; FVC, forced vital capacity; DI, damage index; % Residual damage - calculated for DI after 3 h MMS treatment for each subject taking the value of DI after 1 h MMS treatment as 100%; BI, Binucleated cells; KR, Karyorrhectic cells; CC, Condensed chromatin cells; BUD, Nuclear Buds cells; PY, Pyknotic cells; MN, Micronuclei; KL, Karyolytic cells. Statistical analysis was performed by Spearman Correlation test.

ISSN: 1471-2350