Allele-specific polymerase chain reaction for the detection of Alzheimer’s disease-related single nucleotide polymorphisms

Table 2 Different size of amplicons originated from different SNPs and genotypes

Gene	Genotype	Assay	Interacted primers		Size of amplicons (bp)
Gene	Genotype	Assay	Forward	Reverse	Size of amplicons (bp)
BIN1	AA	Wild type and variant type	BIN1-Fc	BIN1-Rc	767
	AA		BIN1-Fas-W		308
	AG		BIN1-Fc		767
			BIN1-Fas-W		308
			BIN1-Fc	BIN1-Ras-V	492
	GG			BIN1-Rc	767
	GG			BIN1-Ras-V	492
CLU	CC	Wild type and variant type	CLU-Fc	CLU-Rc	685
	CC		CLU-Fas-W		419
	CT		CLU-Fc		685
			CLU-Fas-W		419
			CLU-Fc	CLU-Ras-V	308
	TT		CLU-Fc	CLU-Rc	685
	TT		CLU-Fc	CLU-Ras-V	308
ABCA7	TT	Wild type	ABCA7-Fc	ABCA7-Rc	846
		Wild type	ABCA7-Fas-W		369
		Variant type	ABCA7-Fc		846
	TG	Wild type	ABCA7-Fc		846
		Wild type	ABCA7-Fas-W		369
		Variant type	ABCA7-Fc		846
		Variant type	ABCA7-Fas-V		369
	GG	Wild type	ABCA7-Fc		846
		Variant type	ABCA7-Fc		846
		Variant type	ABCA7-Fas-V		369
CR1	GG	Wild type	CR1-Fc	CR1-Rc	664
		Wild type		CR1-Ras-W	256
		Variant type		CR1-Rc	664
	GA	Wild type		CR1-Rc	664
		Wild type		CR1-Ras-W	256
		Variant type		CR1-Rc	664
		Variant type	CR1-Fas-V		448
	AA	Wild type	CR1-Fc		664
		Variant type	CR1-Fc		664
		Variant type	CR1-Fas-V		448
PICALM	GG	Wild type	PICALM-Fc	PICALM-Rc	718
		Wild type		PICALM-Ras-W	286
		Variant type		PICALM-Rc	718
	GA	Wild type		PICALM-Rc	718
		Wild type		PICALM-Ras-W	286
		Variant type		PICALM-Rc	718
		Variant type	PICALM-Fas-V		481
	AA	Wild type	PICALM-Fc		718
		Variant type	PICALM-Fc		718
		Variant type	PICALM-Fas-V		481

ISSN: 1471-2350