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Table 1 Sequence of primers and the concentration for the genotyping of the polymorphism of BIN1 , CLU , ABCA7 , CR1 and PICALM

From: Allele-specific polymerase chain reaction for the detection of Alzheimer’s disease-related single nucleotide polymorphisms

Gene SNP ID Primer name Sequence (5’-3’) Concentration of primer in AS-PCR (μM)
BIN1 rs744373 BIN1-Fc AAG ACG GAG AGA GGA GGC AT 0.4
BIN1-Rc CCA TCT TCT TCT GCT CTC CCA G 0.1
BIN1-Fas-W CAT GGG CAG CCT CTG AGA 0.1
BIN1-Ras-V AGG CAG GTC TGA GGC C 0.1
CLU rs11136000 CLU-Fc CCT GGC TTA AAG AAT CCA CTC ATC 0.1
CLU-Rc CAG GGG ATT CCT TTG AGA TAG AGT 0.1
CLU-Fas-W GCA AGG GCC CGT TAG AGA A 0.1
CLU-Ras-V CAA AGC CAC ACC AGC TAT CAA AAC 0.1
ABCA7 rs3764650 ABCA7-Fc AAA ATT AGC CAG GCG ACT TGG 0.05
ABCA7-Rc TCA GTG TCA CGG AGT AGA TCC 0.05
ABCA7-Fas-W GCT GCG AAC TTT GCA CCT 0.05
ABCA7-Fas-V GCT GCG AAC TTT GCA CCG 0.05
CR1 rs3818361 CR1-Fc TGC TCC ATA ACC AGT AGT TGA A 0.1
CR1-Rc CAC TCA CCC TTC ATC GCA AA 0.1
CR1-Ras-W TGG GGC AAT TTC CTT TGC 0.4
CR1-Fas-V CCT CTG GTA AGC ATA AGA TAT AA 0.4
PICALM rs3851179 PICALM-Fc TCT ATT TTC TGC CTT ACT GTC 0.04
PICALM-Rc GCT GTT CAG TAA ATC TGA ATT TCT 0.04
PICALM-Ras-W CCA TAT AAT AGT TGT GAT AGA TAA C 0.3
PICALM-Fas-V CAA ACA ATA CAC ACT TCA GTA AAT A 0.04
  1. The specific nucleotides at the 3’-end of primers were underlined.