Table 2 Methylation levels in neuroblastoma cell lines compared to primary tumors
Sample group | APC (% met) | BLU (%) | CASP8- A1 (%) | CASP8- A2 (%) | DAPK (%) | DCR2 (%) | CDH1 (%) | NORE1A (%) | P14 (%) | P16 (%) | TP73 (%) | PTEN (%) | RARB (%) | RASSF1A (%) | RASSF2 (%) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cut-off | |||||||||||||||
Hypermethylation | >10 | >10 | >10 | >50 | >10 | >30 | >10 | >10 | >10 | >10 | >10 | >10 | >10 | >30 | >10 |
NB Cell lines | |||||||||||||||
IMR-32 | 2 | 6 | 77 | 81 | 0 | 80 | 9 | 3 | 5 | 0 | 1 | 1 | - | 93 | 6 |
SH-5YSY | 1 | 36 | 89 | 85 | 0 | 88 | 19 | 2 | 1 | 1 | 1 | 1 | - | 94 | 11 |
SK-N-AS | 1 | - | 0 | 77 | 0 | 81 | 6 | 3 | 2 | 0 | 5 | 1 | - | 94 | 26 |
SK-N-BE | - | - | 94 | - | - | 78 | - | - | - | 1 | - | 0 | - | 95 | - |
SK-N-DZ | 3 | - | 42 | 39 | 1 | 37 | 12 | 6 | 3 | 1 | 3 | 1 | 4 | 92 | 6 |
SK-N-F1 | 1 | - | 50 | 72 | 0 | 76 | 10 | 1 | 2 | 1 | 2 | 1 | 8 | 95 | 10 |
SK-N-SH | 0 | - | 78 | 80 | 0 | 86 | 8 | 2 | - | 1 | 1 | 1 | 3 | 93 | 8 |
All | 1 | 21 | 61 | 73 | 0 | 75 | 11 | 3 | 3 | 1 | 2 | 1 | 5 | 94 | 11 |
NB Tumors | |||||||||||||||
All | 1 | 7 | 16 | 42 | 0 | 12 | 3 | 1 | 1 | 0 | 1 | 1 | 2 | 43 | 6 |
Number > cut-off | n = 0 | n = 4 | n = 21 | n = 9 | n = 0 | n = 4 | n = 1 | n = 0 | n = 0 | n = 0 | n = 0 | n = 0 | n = 0 | n = 23 | n = 1 |