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Figure 2 | BMC Medical Genetics

Figure 2

From: A novel SYBR-based duplex qPCR for the detection of gene dosage: detection of an APC large deletion in a familial adenomatous polyposis patient with an unusual phenotype

Figure 2

X chromosome dosage assay for validation of the SYBR green duplex qPCR. A: Distribution of the normalized HPRT1 / GAPDH ratio in 24 control samples (12 females and 12 males); the normalized ratio of the 12 female samples ranged from 0.90 to 1.09, while in the 12 male samples the range was from 0.63 to 0.70, when using a female sample as the control. B: Visual comparison of the melt curve revealing a reduction of the HPRT1 peak when comparing a male to a female sample.

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BMC Medical Genetics

ISSN: 1471-2350

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