Figure 3

Evaluation of a human choroidal neovascular membrane with anti-P-selectin antibody. Sections of affected eyes were dual labeled with UEA-1 lectin, which binds both normal and neovascular endothelial cells (A), and antibodies directed against P-selectin (B). Panel C shows the merged image with DAPI (blue; UEA-I labeling appears red and anti-P-selectin labeling appears green). Labeling of the vasculature with P-selectin antibody was unremarkable. The extent of the neovascular membrane is indicated by the bracket. Arrows indicate endothelial cells within the neovascular complex and asterisks indicate points along Bruch's membrane. RPE, two layer of dystrophic RPE cells above the neovascular membrane; CHO, choroid.