Figure 2

2OMeAO mediated excision of dystrophin exon 25 in normal and patient cells. 2OMeAOs designed to excise exon 25 from the dystrophin transcript were transfected into normal human myoblasts (A) and MyoD adenovirus converted (c.3385 Insertion A) patient myogenic cells (B). Nested RT-PCR was undertaken across exons 18-26 and densitometry used to quantify the relative levels of full length and exon 25 deleted (Δ25) amplicon in each lane as a measure of exon skipping efficacy. The percentage of exon 25 skipping induced by AOs in both normal (black line) and patient cells (grey line) is presented (C).