Table 1 Primer sequences and their location
Primer | Sequence(5'→3') | GenBank No. | Location Nucleotide(nt) | Product length |
|---|---|---|---|---|
β-540 | FP: tttcccaaaacctaataagtaac RP: aacttcatccacgttcacc | NG_000007 NG_000007 | nt69848-nt69870 nt70647-nt70665 | 818 bp |
Gγ-promoter | FP:tgaaactgttgctttatagga t RP: gagcttattgataacctcagacg | NG_000007 NG_000007 | nt42215-nt42236 nt42850-nt42872 | 657 bp |
Aγ-promoter | FP:ctgctaactgaagagactaagatt RP: caaatcctgagaagcgacct | NG_000007 NG_000007 | nt47259-nt47283 nt47962-nt47981 | 723 bp |
α2-globin gene | FP:tggagggtggagacgtcctg RP: ccattgttggcacattccgg | NG_000006 NG_000006 | nt33537-nt33556 nt34062-nt34621 | 1085 bp |
α1-globin gene | FP:tggagggtggagacgtcctg RP:tccatcccctcctcccgcccctgccttttc | NG_000006 NG_000006 | nt37341-37360 nt38492-38521 | 1180 bp |
BCL11A | FP: tgaggagacccaaacagttaaag RP: aacccacatggcaaccaatag | NT_022184 NT_022184 | nt49880-nt49902 nt50359-nt50379 | 500 bp |
AHSP | FP:tgtcatgtaatagggctcagtaa RP:tggtcactcaaggctgctaac | NW_001838236.1 NW_001838236.1 | nt1217371-nt1217395 nt1218685-nt1218705 | 1335 bp |
HRI | FP: accccgaatatgacgaatc RP: aaggcttactaaatacaacg | NM_014413 NM_014413 | nt166-nt184 nt2034-nt2053 | 1888 bp |
GATA-1 | FP: tgggatcacactgagcttgc RP: gctacaagaggagaaggacacc | NM_002049 NM_002049 | nt10-nt29 nt1365-nt1387 | 1378 bp |