Table 2 LEPR SNP primer and probe sequencies

LEPR c.326A>G

Forward

5'-AgTggTACTCACTTTTCTAACTTATC-3'

Reverse

5'-gAATTAAAAAACATTgTTCAATACA-3'

Sensor

5'-AACATTgAAggAA(A/g)gACATTTgTT-Fluorescein

Anchor

LC640-CAACAgTAAATTCTTTAgTTTTTCAACAAATAgg-Phosphate

LEPR c.668A>G

Forward

5'-CAgCCAAACTCAACgACACT-3'

Reverse

5'-CCACTCTTAATACCCCCAgTACTA-3'

Sensor

5'-CATTAgAggTgAC(T/C)ggAAAATTAC-Fluorescein

Anchor

LC640-CCACCAgATgTgATTTTCAAACACATAAgg-Phosphate

LEPR c.1968G>C

Forward

5'-CAACTTgTCATTTTgCAgTTCCTA-3'

Reverse

5'-gCTTTCCgAAgATTAATAACAggAT-3'

Sensor

5'-TgACATTTTTCTC(C/g)TTTTTCATAgTATC-Fluorescein

Anchor

LC640-CCATTAATTATTCTCCAAAATTCAggTCCT-Phosphate

LEPR c.3024A>G

Forward

5'-CCAgAgACAACCCTTTgTTAAATACg-3'

Reverse

5'-TggTgAAATTATgTTgggATgC-3'

Sensor

5'-gCACTTggTgAC(T/C)gAACTATTTAT-Fluorescein

Anchor

LC640-gCCCTTgTTCTTCACCAgTTTCACTT-Phosphate