Table 1 Description of PCR Assays

Rare allelic form

(amino acid changes)

677C>T (A222V)

2R

[17]

Sense primer and antisense primer

GGGTCAGAAGCATATCAGTCATG

CACAAAGCGGAAGAATGTGTC

AAAAGGCGCGCGGAAG

GCCGGCCACAGGCAT

Annealing temperature, cycle number

55°C, 38 cycles

61°C, 38 cycles

PCR product size in base pairs

326

111 or 139

Restriction enzyme (number of units) or size of ins/del

Hinf I (0.2), buffer 2

28 bp repeat visible by gel electrophoresis

Fragment sizes for common allele in base pairs.

Fragment sizes for rare allele in base pairs.

104 and 222

102, 166 and 53

139

111