Figure 1
From: Mutation screening of NOS1AP gene in a large sample of psychiatric patients and controls
Expression analysis of NOS1AP short and long isoforms in normal control human tissues. One microgram of total RNA from different tissues and specifically from regions of the human brain was reverse transcribed using the Gene Amp RNA PCR kit (Perkin-Elmer Corp., Norwalk, CT) and primers located in exons 1-7, 6-9 and 8-10 (forward -reverse). The ages of the two males and the two females studied were 74, 42, 55, and 36 years-old, with a post-mortem delay of 10, 21, 24, and 2 h, respectively. f: frontal cortex, tc: temporal cortex, o: occipital cortex, h: hippocampus, t: thalamus; c: cerebellum. Normal control human brains were obtained at autopsy under guidelines approved by the ethics committee.