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Table 3 Results of the mutation analyses of KIT exon 11 performed in trial 3.

From: Pitfalls in mutational testing and reporting of common KIT and PDGFRA mutations in gastrointestinal stromal tumors

  Lab A Lab C Lab D Lab E Lab F
Sample T3.1 T3.2 T3.3 T3.1 T3.2 T3.3 T3.1 T3.2 T3.3 T3.1 T3.2 T3.3 T3.1 T3.2 T3.3
Experimental Step A S A S A S A S A S A S A S A S A S A S A S A S A S A S A S
Primer Lab A/B + + + + + + + + + + + + + + + + + + + + + + + +/- + + + + + +
Primer Lab C/D + + + + + + + + + + + + + + + + + + + + + + + +/- + + + + + +
Primer Lab E +/- - +/- - +/- - - - - - - - + + + - + +/- + + + + + +/- + +/- + +/- + +/-
Primer Lab F +/- - + + + +/- + + + + + + + - + + + - + - + + + - + - + + + +
  1. For each lab the result of amplification (A) and sequence analysis (S) is detailed. "+" means success of amplification or nominal result in sequence analysis, "+/-" means only faint amplification with unspecific extra-bands or different/not-scorable sequence-analysis, and "-" means failure of amplification or sequence analysis. Primer pairs used in Lab A and B and Lab C and D were the same, respectively. Lab B did not participate in the third trial suppl.