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Table 2 PCR-RFLP analysis of the SPINK5 gene SNPs

From: A functional polymorphism in the SPINK5 gene is associated with asthma in a Chinese Han Population

Polymorphic Site Primers PCR products Restriction enzyme
-206 G>A 5'-TCTGCCTGCTCCAACTAA-3'
5'-CACTGACACTGTGGCTATCTT-3'
674 bp Kpn I
1103A>G (Asn368Ser) 5'-TCTGCCAATGTAGATGTT-3'
5'-CTCTAATGACTCCCAAGC-3'
670 bp Bts I
1156G>A (Asp386Asn) As above 670 bp Mbo I
1258G>A (Glu420Lys) 5'-TTTTAGCCAAGCAGAAGAAG-3'
5'-CAGTTTTAAGGAATGCACGT-3
177 bp Hinf I
2475G>T(Glu825Asp) 5'-TGGGGAACTGAAGAGCA-3'
5'-GGGACATTATTTTGCCTATC-3'
688 bp BstF5I