Table 2 PCR-RFLP analysis of the SPINK5 gene SNPs
From: A functional polymorphism in the SPINK5 gene is associated with asthma in a Chinese Han Population
Polymorphic Site | Primers | PCR products | Restriction enzyme |
|---|---|---|---|
-206 G>A | 5'-TCTGCCTGCTCCAACTAA-3' 5'-CACTGACACTGTGGCTATCTT-3' | 674 bp | Kpn I |
1103A>G (Asn368Ser) | 5'-TCTGCCAATGTAGATGTT-3' 5'-CTCTAATGACTCCCAAGC-3' | 670 bp | Bts I |
1156G>A (Asp386Asn) | As above | 670 bp | Mbo I |
1258G>A (Glu420Lys) | 5'-TTTTAGCCAAGCAGAAGAAG-3' 5'-CAGTTTTAAGGAATGCACGT-3 | 177 bp | Hinf I |
2475G>T(Glu825Asp) | 5'-TGGGGAACTGAAGAGCA-3' 5'-GGGACATTATTTTGCCTATC-3' | 688 bp | BstF5I |