Figure 1

SNaPshot analysis of the five mutations [C1494T, T1095C, A1555G, 961delT+C(n) and A827G]. Peaks are shown that represent wild-type alleles, for one individual, at all five loci. The smaller non-specific peaks in the figure are all either below a certain threshold peak height value or do not fall into specific bins and are therefore not genotyped by the GeneMapper software. * The SNaPshot extension primers for 961 delT+C(n) and A827G are in the reverse orientation. In the SNaPshot figure, the genotypes are represented as follows: For C1494T, either a black peak (representing the C allele) or a red peak (T allele) is present, in their respective bin positions. For T1095C, either a red peak (T allele) or a black peak (C allele) is present, in their respective bin positions. For A1555G, either a green peak (A allele) or a blue peak (G allele) is present, in their respective bin positions. For 961delT+C(n) (as the primer anneals to the reverse strand), either a green peak (A allele; T on forward strand) or a blue peak (G allele; C on forward strand) is present, in their respective bin positions. For A827G (as the primer anneals to the reverse strand), either a red peak (T allele; A on forward strand) or a black peak (C allele; G on forward strand) is present, in their respective bin positions.