Table 1 Location region, primer sequences, alleles, MAF and restriction enzymes for Emilin1 gene SNPs
SNPs | Emilin1 region | Primer sequences | Alleles Major/Minor | MAF | Restriction enzymes | Digested bands (bp) |
|---|---|---|---|---|---|---|
Rs3754734 | 5'fanking | F: 5' CGCTCTCACCCACAATACCT 3' R: 5' TGGCAAATGGTAAATGCTCA 3' | T/G | 0.367 | Apo I | T allele: 191 G allele: 159 |
Rs2011616 | Intron 1 | F: 5' TTGACCCCATCCAGTAATC 3' R: 5'AATAAGCCATTGATGAGCCTGAC 3' | G/A | 0.356 | Hinf I | G allele: 153 A allele: 129 |
Rs2304682 | Intron 5 | F: 5' TGCTTTGAAGTCCACGTAGCATC 3' R: 5' TGGGAAGGGTGGATGTTA 3' | G/C | 0.367 | Taq I | G allele: 256 C allele: 279 |