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Table 2 Amplicon size, restriction endonucleases used and allele wise expected size of fragments after restriction digestion for the three SNPs studied

From: SLC1A3 C3590T but not BDNF G196A is a predisposition factor for stress as well as depression, in an adolescent eastern Indian population

SNP

Expected amplicon size (bp)

Restriction endonuclease used

Allele-wise expected fragment size (bp)

BDNF G196A

(rs 6265)

274

NlaIII

(5′.... CATG^....3′)

(5′...^GTAC......3’

GG = 216, 58

GA = 216, 139, 77, 58

AA = 139, 77, 58

SLC1A3 C3590T

(rs 2269272)

232

PflF1

(5′..GACN^NNGTC..3′)

(3′..CTGNN^NCAG..5′)

CC = 157, 75

CT = 232, 157, 75

TT = 232

SLC1A3 G869C

(rs 137852619)

223

BstU1

(5′..CG^CG..3′)

(3′..GC^GC..5′)

GG = 223

GC = 223, 152, 71

CC = 152, 71

  1. ^ represent the recognition site